This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
AHR antibody (PA1782-1) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
Filter:
Flow Cytometry analysis of A431 cells using anti-AHR antibody (PA1782-1).
Overlay histogram showing A431 cells stained with PA1782-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AHR Antibody (PA1782-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing A431 cells stained with PA1782-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AHR Antibody (PA1782-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Bundle Save
Anti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®
AHR antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
AHR antibody was positively detected by WB in:
human HELA whole cell, human PC-3 whole cell, human CACO-2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
AHR antibody was positively detected by IHC in:
rat brain tissue, rat brain tissue, mouse brain tissue, mouse brain tissue, human gastric cancer tissue, human lymphadenoma tissue, human placenta tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
AHR antibody was positively detected by ICC/IF in:
A431 cell
AHR antibody was positively detected by FC in:
A431 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Bovine
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®
View all AHR Antibodies
View all AHR Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human AHR.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
100-110 kDa
Calculated Molecular Weight
96.1 kDa
Background of AHR
AHR (aryl hydrocarbon receptor), also called bHLHe76, is a member of the family of basic helix-loop-helix transcription factors. AhR is a cytosolic transcription factor that is normally inactive, bound to several co-chaperones. The AHR gene is mapped on 7p21.1. Estrogenic actions of AHR agonists were detected in wildtype ovariectomized mouse uteri, but were absent in Ahr -/- or Er-alpha -/- ovariectomized mice. Complex assembly and ubiquitin ligase activity of CUL4B (AHR) in vitro and in vivo are dependent on the AHR ligand. In the CUL4B (AHR) complex, ligand-activated AHR acts as a substrate-specific adaptor component that targets sex steroid receptors for degradation. Cd4-positive cells from mice lacking Ahr developed Th17 responses but failed to produce Il22 and did not show enhanced Th17 development. Activation of Ahr during induction of EAE accelerated disease onset and increased pathology in wildtype mice, but not in Ahr -/- mice. The TDO-AHR pathway is active in human brain tumors and is associated with malignant progression and poor survival. Ahr activity within ROR-gamma-t-positive ILC could be induced by dietary ligands such as those contained in vegetables of the family Brassicaceae.
Cite This Product
Anti-Aryl hydrocarbon Receptor/AHR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1782-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Cow
94%
High Homology
Dog
94%
High Homology
Monkey
94%
High Homology
Sheep
82%
Moderate Homology
Rabbit
76%
Moderate Homology
Zebrafish
35%
Low homology
Drosophila
18%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for AHR antibody
Immunohistochemistry (IHC) protocol for AHR antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for AHR antibody
Flow Cytometry (FC) protocol for AHR antibody
Publications
Application:
Species:
A new mechanism involved in cardiovascular senescence induced by environmentally relevant dose of 16 priority-controlled PAHs
Published: 2025-02-14
Journal: ENVIRONMENT INTERNATIONAL
Application: WB
Impact Factor: 9.70
Species: Rat,Human
PMID: 39970779
Kynurenine Attenuates Ulcerative Colitis Mediated by the Aryl Hydrocarbon Receptor
Published: 2024-09-13
Journal: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Application: WB,IF,IHC
Impact Factor: 5.70
Species: Mouse,Human
PMID: 39271472
Indole-3-aldehyde Alleviates High-Fat Diet-Induced Gut Barrier Disruption by Increasing Intestinal Stem Cell Expansion
Published: 2024-08-15
Journal: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Application: WB
Impact Factor: 5.70
Species: Mouse
PMID: 39146439
Aryl hydrocarbon receptor (AhR) is regulated by hyperoxia in premature infants
Published: 2024-05-30
Journal: Journal of Maternal-Fetal & Neonatal Medicine
Application: IF,WB
Impact Factor: 1.70
Species: Human
PMID: 38816997
Resveratrol protects intestinal integrity, alleviates intestinal inflammation and oxidative stress by modulating AhR/Nrf2 pathways in weaned piglets challenged with diquat
Published: 2021-07-22
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 4.93
Species: Pig
PMID: 34303281
AhR activation attenuates calcium oxalate nephrocalcinosis by diminishing M1 macrophage polarization and promoting M2 macrophage polarization
Published: 2020-10-25
Journal: Theranostics
Application: IHC
Impact Factor: 8.58
Species: Mouse
PMID: 33204326
Promoting epithelial-to-mesenchymal transition by d-kynurenine via activating aryl hydrocarbon receptor
Published: 2018-02-13
Journal: MOLECULAR AND CELLULAR BIOCHEMISTRY
Application: WB
Impact Factor: 2.56
Species: Human
PMID: 29442266
Review
Have you used Anti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-Aryl hydrocarbon Receptor/AHR Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PA1782-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026