Anti-Aryl hydrocarbon Receptor/Ahr Antibody Picoband™

Product Name

Anti-Aryl hydrocarbon Receptor/Ahr Antibody Picoband™

View all AHR Antibodies

SKU/Catalog Number

A00225-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Aryl hydrocarbon Receptor/Ahr Antibody Picoband™ catalog # A00225-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Aryl hydrocarbon Receptor/Ahr Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00225-3)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived rat Aryl hydrocarbon Receptor/Ahr recombinant protein (Position: R15-Q196).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00225-3 is reactive to AHR in Mouse, Rat

Applications

A00225-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

110 kDa

Calculated molecular weight

96.147kDa

Background of AHR

AHR (aryl hydrocarbon receptor), also called bHLHe76, is a member of the family of basic helix-loop-helix transcription factors. AhR is a cytosolic transcription factor that is normally inactive, bound to several co-chaperones. The AHR gene is mapped on 7p21.1. Estrogenic actions of AHR agonists were detected in wildtype ovariectomized mouse uteri, but were absent in Ahr -/- or Er-alpha -/- ovariectomized mice. Complex assembly and ubiquitin ligase activity of CUL4B (AHR) in vitro and in vivo are dependent on the AHR ligand. In the CUL4B (AHR) complex, ligand-activated AHR acts as a substrate-specific adaptor component that targets sex steroid receptors for degradation. Cd4-positive cells from mice lacking Ahr developed Th17 responses but failed to produce Il22 and did not show enhanced Th17 development. Activation of Ahr during induction of EAE accelerated disease onset and increased pathology in wildtype mice, but not in Ahr -/- mice. The TDO-AHR pathway is active in human brain tumors and is associated with malignant progression and poor survival. Ahr activity within ROR-gamma-t-positive ILC could be induced by dietary ligands such as those contained in vegetables of the family Brassicaceae. 

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Mouse, Rat
Flow Cytometry, 1-3μg/1x10⁶ cells, Rat
Direct ELISA, 0.1-0.5μg/ml, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Ahr using anti-Ahr antibody (A00225-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat PC-12 whole cell lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: rat RH35 whole cell lysates,
Lane 5: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ahr antigen affinity purified polyclonal antibody (Catalog # A00225-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ahr at approximately 110KD. The expected band size for Ahr is at 96KD.

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Figure 2. Flow Cytometry analysis of Neuro-2a cells using anti-Ahr antibody (A00225-3).
Overlay histogram showing Neuro-2a cells stained with A00225-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Ahr Antibody (A00225-3, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For AHR (Source: Uniprot.org, NCBI)

Alternative Names

Ah receptor; AHR; AH-receptor; aryl hydrocarbon receptor; BHLHE76; bHLHe76aromatic hydrocarbon receptor; Class E basic helix-loop-helix protein 76 AHR RP85, bHLHe76 aryl hydrocarbon receptor aryl hydrocarbon receptor|AH-receptor|ah receptor|aromatic hydrocarbon receptor|class E basic helix-loop-helix protein 76

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