Human ESM1/Endocan ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ESM1/Endocan. 96wells/kit, with removable strips. Cited in 4 publication(s).
Human ESM1/Endocan ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Human ESM1/Endocan ELISA Kit PicoKine™
See all Endocan/ESM-1 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human Endocan/ESM1 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Endocan/ESM1 tissue specificity: Expressed in lung, on the vascular capillary network within alveolar walls, and also at lower level in kidney.|
|Cite This Product||Human ESM1/Endocan ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0752)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Immunogen/Standard||Expression system for standard: NSO; Immunogen sequence: W20-R184|
|Antibody Clonalities||Capture antibody|Detection antibody
polyclonal antibody from goat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For ESM1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||11082|
|Species Of This Entry||Human|
|Protein Name||Endothelial cell-specific molecule 1|
|Alternative Names||Endocan/ESM-1|Endocan; endothelial cell-specific molecule 1; ESM1; ESM-1; IGFBP-rp6|
|Post Tranlational Modifications||Phosphorylation; Glycosylation; Cleavage|
|Gene Location||5q11.2, on Chromasome 5, gene sequence: NC_000005.10 (54977867..54985593, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For ESM1 (Source: Uniprot.org, NCBI)
|Protein Function||Involved in angiogenesis; promotes angiogenic sprouting. May have potent implications in lung endothelial cell-leukocyte interactions.|
|Tissue Specificity||Expressed in lung, on the vascular capillary network within alveolar walls, and also at lower level in kidney.|
|Related Diseases||Neoplasms; Malignant Neoplasms; Tumor Angiogenesis; Tissue Adhesions; Neoplasm Metastasis; Carcinoma; Inflammation; Pathologic Neovascularization; Malignant Neoplasm Of Lung; Cell Invasion; Lung Neoplasms; Adenocarcinoma; Cell Transformation, Neoplastic; Systemic Infection; Liver Neoplasms; Kidney Neoplasm; Immunologic Deficiency Syndromes; Liver Carcinoma; Neoplasm Invasiveness|
|Related Pathways||Angiogenesis; Cell Proliferation; Cell Adhesion; Cell Migration; Secretion; Cell Cycle; Cell Growth; Pathogenesis; Cell Activation; Tube Formation; Cell Cycle Arrest; Endothelial Cell Activation; Gene Silencing; Vasculogenesis; Lymphangiogenesis; Endothelial Cell Proliferation; Inflammatory Response; Oviposition; Cell Motility; Cell Differentiation|
|Background||Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF(vascular endothelial growth factor) or FGF-2 (fibroblast growth factor 2).|
|Scientific References||PMID: 8702785 by Lassalle P., et al. ESM-1 is a novel human endothelial cell-specific molecule expressed in lung and regulated by cytokines.|
PMID: 20616313 by Brutsch R., et al. Integrin cytoplasmic domain-associated protein-1 attenuates sprouting angiogenesis.
Kit Components And Assay QC Data Details Of Human ESM1/Endocan ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0752-CAP||96-well plate precoated with anti-Human Endocan/ESM-1 antibody||1|
|EK0752-ST||lyophilized recombinant Human Endocan/ESM-1 standard||10ng/tube|
|EK0752-DA||biotinylated anti-Human Endocan/ESM-1 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0752 Human ESM1/Endocan ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human ESM1/Endocan ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human ESM1/Endocan ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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Total number of citations: 4
|Plasma endocan level and prognosis of immunoglobulin A nephropathy||PubMed ID 27668158|
|Endocan and Non-Dipping Circadian Pattern in Newly Diagnosed Essential Hypertension||PubMed ID 27826342|
|Serum markers for asymptomatic atherosclerosis in Egyptian psoriatic patients: study controlled by Doppler estimation of carotid intima-media thickness||PubMed ID 30022835|
|Higher Serum Endocan Level Is Associated with Alzheimer Disease||PubMed ID 29393258|
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