Human ESM1/Endocan ELISA Kit PicoKine®

Endocan/ESM-1 ELISA kit for Human

Human ESM1/Endocan ELISA Kit PicoKine™ (96 Tests). Quantitate Human ESM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 10 publication(s).

Product Info Summary

SKU: EK0752
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human ESM1/Endocan ELISA Kit PicoKine®

View all Endocan/ESM-1 ELISA kits

SKU/Catalog Number



96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?


Human ESM1/Endocan ELISA Kit PicoKine™ (96 Tests). Quantitate Human ESM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human ESM1/Endocan ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0752)

Clonality of Antibodies

See Datasheet for details


Expression system for standard: NS0; Immunogen sequence: W20-R184


<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0752 is reactive to ESM1 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0752 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Endocan/ESM-1

Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF (vascular endothelial growth factor) or FGF-2 (fibroblast growth factor 2).

Kit Components

Catalog Number Description Quantity
EK0752-CAP Anti-Human ESM1 Pre-coated 96-well strip microplate 1
EK0752-ST Human ESM1 Standard 2 vials, 10 ng/tube
EK0752-DA Human ESM1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Endocan/ESM-1, Dilution ratio of 1:1, concentration in serum is 200-800pg/ml.

Some articles we found to cite concentrations of Endocan/ESM-1 in samples: 24176522, 24108208, 21958978, 11025405, 25056533 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human ESM1/Endocan ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Mean (pg/ml)9026983296293860
Standard deviation7.1114.7945.768.4419.3354.18
CV (%)7.9%5.5%5.5%8.8%6.6%6.3%


We ensure reproducibility by testing three samples with differing concentrations of Endocan/ESM-1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 190838380843.674.3%
Sample 226923526922224820.768.3%
Sample 383281592682584944.575.2%
*number of samples for each test n=16.

Gene/Protein Information For ESM1 (Source: Uniprot.Org, NCBI)

Uniprot ID


Gene ID


Gene Name


Full Name

Endothelial cell-specific molecule 1



Alternative Names

Endocan; endothelial cell-specific molecule 1; ESM1; ESM-1; IGFBP-rp6 ESM1 endocan endothelial cell specific molecule 1 endothelial cell-specific molecule 1|ESM-1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ESM1, check out the ESM1 Infographic

ESM1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ESM1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0752 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Serum Endocan Levels Predict Drug-Eluting Stent Restenosis in Patients with Stable Angina Pectoris

Admission Endocan Level may be a Useful Predictor for In-Hospital Mortality and Coronary Severity Index in Patients With ST-Segment Elevation Myocardial Infarction:

Human Endothelial Cell-Specific Molecule-1 (Endocan) and Coronary Artery Disease and Microvascular Angina:

Higher Serum Endocan Level Is Associated with Alzheimer Disease

Subclinical atherosclerosis in psoriatic disease: relation to endocan, TNF‐α, age of onset, and body fat

Serum endocan as a predictive biomarker of cardiovascular risk in erectile dysfunction patients

Endocan as a marker of microvascular inflammation in kidney transplant recipients

Endocan: A novel biomarker for risk stratification, prognosis and therapeutic monitoring in human cardiovascular and renal diseases

Serum levels of endocan and endoglin are associated with large-artery atherosclerotic stroke

Higher Serum Endocan Level Is Associated with Alzheimer Disease

Have you used Human ESM1/Endocan ELISA Kit PicoKine®?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Human ESM1/Endocan ELISA Kit PicoKine®

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

9 Customer Q&As for Human ESM1/Endocan ELISA Kit PicoKine®


Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Endocan using the EK0752 Human Endocan Picokine® ELISA Kit?

C. Mehta

Verified customer

Asked: 2020-06-27


A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2020-06-27


Q: how to thaw whole blood sample for Endocan ELISA after freezing?

Verified Customer

Verified customer

Asked: 2019-04-01


A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Endocan for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2019-04-01


Q: if the enzyme conjugated Endocan antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Endocan antigen?

Verified Customer

Verified customer

Asked: 2018-11-13


A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-11-13


Q: how to analyze ELISA data? I measured Endocan level in cell lysates.

Verified Customer

Verified customer

Asked: 2018-07-31


A: we recommend you this article on ELISA data analysis. Boster also provides a free tool that you can use to analyze ELISA data.

Boster Scientific Support

Answered: 2018-07-31


What proteins have EK0752 been tested for cross-reactivity?

Verified Customer

Verified customer

Asked: 2017-10-02


We tested EK0752 for recombinant human IL-1 beta, TNF-α, VEGF, and HGF.

Boster Scientific Support

Answered: 2017-10-02


Q: can I use citrate plasma as samples in Human Endocan Picokine® ELISA Kit (Catalog # EK0752)?

Verified Customer

Verified customer

Asked: 2017-03-31


A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Endocan causing disruption of its protein structure. Endocan may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Endocan ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2017-03-31


Q: Can Endocan ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

H. Banerjee

Verified customer

Asked: 2016-12-10


A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2016-12-10


Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2015-02-03


A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2015-02-03


Can you provide a reference value for endocan?

Verified Customer

Verified customer

Asked: 2015-02-02


The Endocan level is about 0.92ng/ml in serum and 1.5ng/ml in plasma.

Boster Scientific Support

Answered: 2015-02-02


Ask a question

Get A Quote

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product



Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.