Click image to see more details
Product Info Summary
| SKU: | A00558-3 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-AIRE (P126) Antibody
SKU/Catalog Number
A00558-3
Size
100ul
Form
Liquid
Description
Target/process: Autoimmune regulator (AIRE); page associates product with “Central Tolerance Induction” and “Autoimmune Polyendocrinopathy Syndrome, Type 1” (APS-1) categories (well-established AIRE biology). Assay context: rabbit polyclonal primary; applications WB/IHC/IF; reactive species human/mouse/rat; immunogen aa101–150 of human AIRE; calculated MW ~57.7 kDa; suggested dilutions WB 1:500–1:1000, IHC/IF 1:50–1:200. Often co-profiled with APC costimulatory marker CD86 in tolerance/antigen-presentation studies (putative).
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AIRE (P126) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00558-3)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 101-150 of Human AIRE.
Reactive Species
A00558-3 is reactive to AIRE in Human, Mouse, Rat
Calculated molecular weight
57.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00558-3 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Western blot (WB) analysis of AIRE (P126) pAb at 1:500 dilution Lane1:Panc1 whole cell lysate(40ug) Lane2:A2780 whole cell lysate(40ug) Lane3:The Heart tissue lysate of Rat(40ug) Lane4:The Heart tissue lysate of Mouse(40ug)
Click image to see more details
Immunohistochemistry (IHC) analyzes of AIRE (P126) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasmic and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Click image to see more details
Immunohistochemistry (IHC) analyzes of AIRE (P126) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50,showing cytoplasmic and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Specific Publications For Anti-AIRE (P126) Antibody (A00558-3)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-AIRE (P126) Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-AIRE (P126) Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
