Product Info Summary
SKU: | PB10035 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-AKR1B1 Antibody Picoband™
SKU/Catalog Number
PB10035
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-AKR1B1 Antibody Picoband™ catalog # PB10035. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AKR1B1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10035)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human AKR1B1 recombinant protein (Position: L228-F316). Human AKR1B1 shares 87.5% amino acid (aa) sequence identity with both mouse and rat AKR1B1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB10035 is reactive to AKR1B1 in Human, Mouse, Rat
Applications
PB10035 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
36 kDa
Calculated molecular weight
35.853kDa
Background of AKR1B1
Aldo-keto reductase family 1, member B1 (aldose reductase), also known as AR, is an enzyme that in humans is encoded by the AKR1B1 gene. This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number ofaldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of AKR1B1 using anti-AKR1B1 antibody (PB10035).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: rat testis tissue lysates,
Lane 3: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKR1B1 antigen affinity purified polyclonal antibody (Catalog # PB10035) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKR1B1 at approximately 36 kDa. The expected band size for AKR1B1 is at 36 kDa.
Click image to see more details
Figure 2. IHC analysis of AKR1B1 using anti-AKR1B1 antibody (PB10035).
AKR1B1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AKR1B1 Antibody (PB10035) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of AKR1B1 using anti-AKR1B1 antibody (PB10035).
AKR1B1 was detected in paraffin-embedded section of rat adrenal gland tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AKR1B1 Antibody (PB10035) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of AKR1B1 using anti-AKR1B1 antibody (PB10035).
AKR1B1 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AKR1B1 Antibody (PB10035) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. Flow Cytometry analysis of U20S cells using anti-AKR1B1 antibody (PB10035).
Overlay histogram showing U20S cells stained with PB10035 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AKR1B1 Antibody (PB10035,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For AKR1B1 (Source: Uniprot.org, NCBI)
Gene Name
AKR1B1
Full Name
Aldo-keto reductase family 1 member B1
Weight
35.853kDa
Superfamily
aldo/keto reductase family
Alternative Names
ADR; Aldehyde reductase; Aldo-keto reductase family 1 member B1; aldo-keto reductase family 1, member B1 (aldose reductase); ALDR1aldose reductase; ALR2; ARaldehyde reductase 1; EC 1.1.1; EC 1.1.1.21; Lii5-2 CTCL tumor antigen; low Km aldose reductase; MGC1804 AKR1B1 ADR, ALDR1, ALR2, AR aldo-keto reductase family 1 member B aldo-keto reductase family 1 member B1|Lii5-2 CTCL tumor antigen|aldehyde reductase 1|aldose reductase|low Km aldose reductase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AKR1B1, check out the AKR1B1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AKR1B1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AKR1B1 Antibody Picoband™ (PB10035)
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Customer Q&As
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3 Customer Q&As for Anti-AKR1B1 Antibody Picoband™
Question
Is a blocking peptide available for product anti-AKR1B1 antibody (PB10035)?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
We do provide the blocking peptide for product anti-AKR1B1 antibody (PB10035). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-11
Question
We are currently using anti-AKR1B1 antibody PB10035 for rat tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-18
Answer
The anti-AKR1B1 antibody (PB10035) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-18
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetus using anti-AKR1B1 antibody PB10035. Let me know if you need anything else.
N. Baker
Verified customer
Asked: 2014-05-05
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-05-05