Anti-AKR1B1 Antibody Picoband™

AKR1B1 antibody

Boster Bio Anti-AKR1B1 Antibody Picoband™ catalog # PB10035. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB10035
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IHC, WB

Product Name

Anti-AKR1B1 Antibody Picoband™

View all AKR1B1 Antibodies

SKU/Catalog Number

PB10035

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-AKR1B1 Antibody Picoband™ catalog # PB10035. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AKR1B1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10035)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human AKR1B1 recombinant protein (Position: L228-F316). Human AKR1B1 shares 87.5% amino acid (aa) sequence identity with both mouse and rat AKR1B1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB10035 is reactive to AKR1B1 in Human, Mouse, Rat

Applications

PB10035 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

36 kDa

Calculated molecular weight

35.853kDa

Background of AKR1B1

Aldo-keto reductase family 1, member B1 (aldose reductase), also known as AR, is an enzyme that in humans is encoded by the AKR1B1 gene. This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number ofaldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For AKR1B1 (Source: Uniprot.org, NCBI)

Gene Name

AKR1B1

Full Name

Aldo-keto reductase family 1 member B1

Weight

35.853kDa

Superfamily

aldo/keto reductase family

Alternative Names

ADR; Aldehyde reductase; Aldo-keto reductase family 1 member B1; aldo-keto reductase family 1, member B1 (aldose reductase); ALDR1aldose reductase; ALR2; ARaldehyde reductase 1; EC 1.1.1; EC 1.1.1.21; Lii5-2 CTCL tumor antigen; low Km aldose reductase; MGC1804 AKR1B1 ADR, ALDR1, ALR2, AR aldo-keto reductase family 1 member B aldo-keto reductase family 1 member B1|Lii5-2 CTCL tumor antigen|aldehyde reductase 1|aldose reductase|low Km aldose reductase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on AKR1B1, check out the AKR1B1 Infographic

AKR1B1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for AKR1B1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-AKR1B1 Antibody Picoband™

Question

Is a blocking peptide available for product anti-AKR1B1 antibody (PB10035)?

Verified Customer

Verified customer

Asked: 2019-06-11

Answer

We do provide the blocking peptide for product anti-AKR1B1 antibody (PB10035). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-11

Question

We are currently using anti-AKR1B1 antibody PB10035 for rat tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-01-18

Answer

The anti-AKR1B1 antibody (PB10035) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-01-18

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetus using anti-AKR1B1 antibody PB10035. Let me know if you need anything else.

N. Baker

Verified customer

Asked: 2014-05-05

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-05-05

Size

Conjugation

Total: $370

SKU:PB10035

In stock, 1 left.

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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