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Product Info Summary
| SKU: | PB9150 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-NFkB/NFKB2 p100/p52 Antibody Picoband®
SKU/Catalog Number
PB9150
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NFkB/NFKB2 p100/p52 Antibody Picoband® catalog # PB9150. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NFkB/NFKB2 p100/p52 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9150)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NFkB p100/p52 recombinant protein (Position: M1-R340). Human NFkB p100/p52 shares 96% amino acid (aa) sequence identity with mouse NFkB p100/p52.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9150 is reactive to NFKB2 in Human, Mouse, Rat
Observed Molecular Weight
52 kDa (active form), 120 kDa (precursor)
Calculated molecular weight
96.7 kDa
Background of NFKB2
NFKB2, also known as nuclear factor NF-kappa-B p100 subunit, is a protein that in humans is encoded by the NFKB2 gene. It is mapped to 10q24.32. This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFKB). NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9150 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Positive Control
WB: human Hela whole cell, human Jurkat whole cell, human MCF-7 whole cell, human A549 whole cell, human U87 whole cell, mouse lung tissue, mouse RAW2647 whole cell
IHC: rat intestine tissue, human lung cancer tissue, human mammary cancer tissue, mouse intestine tissue
Validation Images & Assay Conditions
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Western blot analysis of NFkB/NFKB2 p100/p52 using anti-NFkB/NFKB2 p100/p52 antibody (PB9150).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human U87 whole cell lysates,
Lane 6: mouse lung tissue lysates,
Lane 7: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFkB/NFKB2 p100/p52 antigen affinity purified polyclonal antibody (Catalog # PB9150) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFkB/NFKB2 p100/p52 at approximately 52 kDa (active form), 120kDa (precursor). The expected band size for NFkB/NFKB2 p100/p52 is at 97 kDa.
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IHC analysis of NFkB/NFKB2 p100/p52 using anti-NFkB/NFKB2 p100/p52 antibody (PB9150).
NFkB/NFKB2 p100/p52 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NFkB/NFKB2 p100/p52 Antibody (PB9150) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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IHC analysis of NFkB/NFKB2 p100/p52 using anti-NFkB/NFKB2 p100/p52 antibody (PB9150).
NFkB/NFKB2 p100/p52 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NFkB/NFKB2 p100/p52 Antibody (PB9150) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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IHC analysis of NFkB/NFKB2 p100/p52 using anti-NFkB/NFKB2 p100/p52 antibody (PB9150).
NFkB/NFKB2 p100/p52 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NFkB/NFKB2 p100/p52 Antibody (PB9150) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
IHC analysis of NFkB/NFKB2 p100/p52 using anti-NFkB/NFKB2 p100/p52 antibody (PB9150).
NFkB/NFKB2 p100/p52 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NFkB/NFKB2 p100/p52 Antibody (PB9150) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-NFkB/NFKB2 p100/p52 Antibody Picoband® (PB9150)
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1 Customer Q&As for Anti-NFkB/NFKB2 p100/p52 Antibody Picoband®
Question
We are currently using anti-NFkB p100/p52/NFKB2 antibody PB9150 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-01
Answer
The anti-NFkB p100/p52/NFKB2 antibody (PB9150) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-01
