Anti-Akt phospho S473 Monoclonal Antibody

Akt1 antibody

Boster Bio Anti-Akt phospho S473 Monoclonal Antibody (Catalog # MP00024). Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey. Cited in 10 publication(s).

Product Info Summary

SKU: MP00024
Size: 100ug
Reactive Species: Human, Monkey, Mouse, Rat
Host: Mouse
Application: ELISA, IF, IHC, WB

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Product Name

Anti-Akt phospho S473 Monoclonal Antibody

View all Akt1 Antibodies

SKU/Catalog Number

MP00024

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Akt phospho S473 Monoclonal Antibody (Catalog # MP00024). Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey.

Storage & Handling

Store Anti-AKT pS473 (MOUSE) Monoclonal Antibody at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Akt phospho S473 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP00024)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 17F6.B11

Isotype

IgG1 kappa

Immunogen

Anti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein, followed by hybridoma development.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

MP00024 is reactive to AKT1 in Human, Monkey, Mouse, Rat

Applications

MP00024 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

55.686kDa

Background of Akt1

AKT phospho S473 is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT pS473 (MOUSE) Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000
Flow Cytometry: User optimized
IHC: 20 µg/mL
IF Microscopy: 1:500-1:3,000
IP: User optimized
WB: 1:500-1:3,000

Validation Images & Assay Conditions

Gene/Protein Information For AKT1 (Source: Uniprot.org, NCBI)

Gene Name

AKT1

Full Name

RAC-alpha serine/threonine-protein kinase

Weight

55.686kDa

Superfamily

protein kinase superfamily

Alternative Names

AKT serine/threonine kinase 1; AKT; Akt1; AKT1m; CWS6; EC 2.7.11; EC 2.7.11.1; PKB alpha; PKB; PKBMGC99656; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; rac protein kinase alpha; RAC; RAC-alpha serine/threonine-protein kinase; RAC-alpha; RAC-PK-alpha; RACPKB-ALPHA; Serine-Threonine Protein Kinase ; v-akt murine thymoma viral oncogene homolog 1; V-Akt Murine Thymoma Viral Oncogene-Like Protein 1 AKT1 AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA AKT serine/threonine kinase 1 RAC-alpha serine/threonine-protein kinase|AKT1m|PKB alpha|RAC-PK-alpha|protein kinase B alpha|proto-oncogene c-Akt|rac protein kinase alpha|serine-threonine protein kinase|v-akt murine thymoma viral oncogene homolog 1|v-akt murine thymoma viral oncogene-like protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on AKT1, check out the AKT1 Infographic

AKT1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for AKT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

MP00024 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Insulin-like growth factor 2 mRNA-binding protein 1 promotes cell proliferation via activation of AKT and is directly targeted by microRNA-494 in pancreatic cancer

A small interfering RNA targeting osteopontin as gastric cancer therapeutics

Short hairpin RNA targeting AKT1 and PI3K/p85 suppresses the proliferation and self-renewal of lung cancer stem cells

Protective Effects of Sodium (?)-5-Bromo-2-(%u03B1-Hydroxypentyl) Benzoate in a Rodent Model of Global Cerebral Ischemia

Vibration exercise decreases insulin resistance and modulates the insulin signaling pathway in a type 2 diabetic rat model

Epigallocatechin-3-Gallate Promotes the Growth of Mink Hair Follicles Through Sonic Hedgehog and Protein Kinase B Signaling Pathways

Hyperthermia induced HIF-1a expression of lung cancer through AKT and ERK signaling pathways

Epicatechin Plus Treadmill Exercise are Neuroprotective Against Moderate-stage Amyloid Precursor Protein/Presenilin 1 Mice

Progesterone alleviates hypoxic-ischemic brain injury via the Akt/GSK-3? signaling pathway

Li R, Cui K, Liu K, Li H, Zhang Y, Liu X, Chen R, Li M, Wang T, Wang S, Liu J, Rao K. Sci Rep. 2017 Oct 18;7(1):13464. doi: 10.1038/s41598-017-12907-1. Metabolic syndrome in rats is associated with erectile dysfunction by impairing PI3K/Akt/eNOS a...

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15 Customer Q&As for Anti-Akt phospho S473 Monoclonal Antibody

Question

Does anti-Akt phospho S473 Monoclonal antibody MP00024 work for IHC with foreskin?

Verified Customer

Verified customer

Asked: 2020-04-30

Answer

According to the expression profile of foreskin, AKT1 is highly expressed in foreskin. So, it is likely that anti-Akt phospho S473 Monoclonal antibody MP00024 will work for IHC with foreskin.

Boster Scientific Support

Answered: 2020-04-30

Question

I was wanting to use your anti-Akt phospho S473 Monoclonal antibody for IHC for mouse foreskin on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse foreskin identification?

D. Collins

Verified customer

Asked: 2020-02-07

Answer

You can see on the product datasheet, MP00024 anti-Akt phospho S473 Monoclonal antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse foreskin in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-07

Question

I see that the anti-Akt phospho S473 Monoclonal antibody MP00024 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-11-06

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-11-06

Question

Is a blocking peptide available for product anti-Akt phospho S473 Monoclonal antibody (MP00024)?

Verified Customer

Verified customer

Asked: 2019-10-31

Answer

We do provide the blocking peptide for product anti-Akt phospho S473 Monoclonal antibody (MP00024). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-10-31

Question

Do you have a BSA free version of anti-Akt phospho S473 Monoclonal antibody MP00024 available?

Verified Customer

Verified customer

Asked: 2019-08-16

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Akt phospho S473 Monoclonal antibody MP00024 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-16

Question

Is this MP00024 anti-Akt phospho S473 Monoclonal antibody reactive to the isotypes of AKT1?

Verified Customer

Verified customer

Asked: 2019-06-04

Answer

The immunogen of MP00024 anti-Akt phospho S473 Monoclonal antibody is anti-AKT pS473 (MOUSE) Monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein, followed by hybridoma development. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-04

Question

We bought anti-Akt phospho S473 Monoclonal antibody for WB on adrenal gland in the past. I am using rat, and We are going to use the antibody for IHC next. My lab would like examining adrenal gland as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2019-05-22

Answer

I viewed the website and datasheets of our anti-Akt phospho S473 Monoclonal antibody and it appears that MP00024 has been tested on rat in both WB and IHC. Thus MP00024 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-05-22

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for foreskin using anti-Akt phospho S473 Monoclonal antibody MP00024. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-02-06

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-02-06

Question

We are currently using anti-Akt phospho S473 Monoclonal antibody MP00024 for rat tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2018-06-25

Answer

The anti-Akt phospho S473 Monoclonal antibody (MP00024) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-06-25

Question

I have a question about product MP00024, anti-Akt phospho S473 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-02-16

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MP00024 anti-Akt phospho S473 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-02-16

Question

Our lab were happy with the WB result of your anti-Akt phospho S473 Monoclonal antibody. However we have seen positive staining in liver cytoplasm. using this antibody. Is that expected? Could you tell me where is AKT1 supposed to be expressed?

J. Carter

Verified customer

Asked: 2018-01-31

Answer

Based on literature, liver does express AKT1. Generally AKT1 expresses in cytoplasm. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-01-31

Question

We have seen staining in rat muscle ovary. Any tips? Is anti-Akt phospho S473 Monoclonal antibody supposed to stain muscle ovary positively?

Verified Customer

Verified customer

Asked: 2017-06-12

Answer

According to literature muscle ovary does express AKT1. According to Uniprot.org, AKT1 is expressed in left adrenal gland, adrenal gland, muscle ovary, foreskin, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-06-12

Question

Please see the WB image, lot number and protocol we used for foreskin using anti-Akt phospho S473 Monoclonal antibody MP00024. Please let me know if you require anything else.

W. Gonzalez

Verified customer

Asked: 2016-04-05

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-04-05

Question

Would MP00024 anti-Akt phospho S473 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

R. Li

Verified customer

Asked: 2014-11-13

Answer

It shows on the product datasheet, MP00024 anti-Akt phospho S473 Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-11-13

Question

We want to test anti-Akt phospho S473 Monoclonal antibody MP00024 on mouse foreskin for research purposes, then I may be interested in using anti-Akt phospho S473 Monoclonal antibody MP00024 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

G. Moore

Verified customer

Asked: 2013-01-31

Answer

The products we sell, including anti-Akt phospho S473 Monoclonal antibody MP00024, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-01-31

Size

Total: $612

SKU:MP00024

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Lead time for this item is typically 4-6 days

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MP00024
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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