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Product Info Summary
| SKU: | M00207-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Rho RHOA Rabbit Monoclonal Antibody
SKU/Catalog Number
M00207-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Rho RHOA Rabbit Monoclonal Antibody catalog # M00207-2. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rho RHOA Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00207-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GOE-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Rho
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00207-2 is reactive to RHOA in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
103 kDa
Calculated molecular weight
21768 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00207-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
IHC: human thyroid cancer tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human thyroid cancer, using Rho Antibody.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of Rho expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For RHOA (Source: Uniprot.org, NCBI)
Gene Name
RHOA
Full Name
Transforming protein RhoA
Weight
21768 MW
Superfamily
small GTPase superfamily
Alternative Names
Transforming protein RhoA;Rho cDNA clone 12;h12;RHOA;ARH12, ARHA, RHO12; RHOA ARH12, ARHA, EDFAOB, RHO12, RHOH12 ras homolog family member A transforming protein RhoA|Aplysia ras-related homolog 12|epididymis secretory sperm binding protein|oncogene RHO H12|small GTP binding protein RhoA
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RHOA, check out the RHOA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RHOA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rho RHOA Rabbit Monoclonal Antibody (M00207-2)
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Customer Q&As
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1 Customer Q&As for Anti-Rho RHOA Rabbit Monoclonal Antibody
Question
We are currently using anti-Rho Rabbit Monoclonal antibody M00207-2 for mouse tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
The anti-Rho Rabbit Monoclonal antibody (M00207-2) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-08
