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Product Info Summary
| SKU: | M00520-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-AKT3 Mouse Monoclonal Antibody [Clone ID: OTI9B2]
SKU/Catalog Number
M00520-1
Size
100 µl
Description
Boster Bio AKT3 mouse monoclonal antibody, clone OTI9B2 (formerly 9B2). Catalog# M00520-1. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-AKT3 Mouse Monoclonal Antibody [Clone ID: OTI9B2] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00520-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI9B2
Isotype
IgG3
Immunogen
Full length human recombinant protein of human AKT3 (NP_005456) produced in E.coli.
Reactive Species
M00520-1 is reactive to AKT3 in Human, Mouse, Rat
Calculated molecular weight
55.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00520-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500
IHC 1:50
IF 1:100
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3.
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Figure from citation: Western Blot of Akt3 protein level by using anti-Akt3 antibody in rat endometrium during pregnancy and pseudopregnancy.
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AKT3 antibody ( 9B2 ) at 1:500 dilution + BALB/C 3T3 cell lysate
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Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-AKT3 monoclonal antibody at 1:200 dilution.
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Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-AKT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either AKT3 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AKT3 antibody (M00520-1)
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Anti-AKT3 mouse monoclonal antibody (M00520-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT3.
Specific Publications For Anti-AKT3 Mouse Monoclonal Antibody [Clone ID: OTI9B2] (M00520-1)
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