Product Info Summary
SKU: | M00520-1 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-AKT3 Mouse Monoclonal Antibody [Clone ID: OTI9B2]
SKU/Catalog Number
M00520-1
Size
100 µl
Description
Boster Bio AKT3 mouse monoclonal antibody, clone OTI9B2 (formerly 9B2). Catalog# M00520-1. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-AKT3 Mouse Monoclonal Antibody [Clone ID: OTI9B2] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00520-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI9B2
Isotype
IgG3
Immunogen
Full length human recombinant protein of human AKT3 (NP_005456) produced in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00520-1 is reactive to AKT3 in Human, Mouse, Rat
Applications
M00520-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
55.775kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3.
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Figure from citation: Western Blot of Akt3 protein level by using anti-Akt3 antibody in rat endometrium during pregnancy and pseudopregnancy.
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AKT3 antibody ( 9B2 ) at 1:500 dilution + BALB/C 3T3 cell lysate
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Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-AKT3 monoclonal antibody at 1:200 dilution.
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Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-AKT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either AKT3 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AKT3 antibody (M00520-1)
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Anti-AKT3 mouse monoclonal antibody (M00520-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT3.
Protein Target Info & Infographic
Gene/Protein Information For AKT3 (Source: Uniprot.org, NCBI)
Gene Name
AKT3
Full Name
RAC-gamma serine/threonine-protein kinase
Weight
55.775kDa
Superfamily
protein kinase superfamily
Alternative Names
Akt3; Akt-3; EC 2.7.11; EC 2.7.11.1; PKB gamma; PKBGDKFZp434N0250; PRKBG; RAC-gamma; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) AKT3 MPPH, MPPH2, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2 AKT serine/threonine kinase 3 RAC-gamma serine/threonine-protein kinase|PKB gamma|RAC-gamma serine/threonine protein kinase|v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AKT3, check out the AKT3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AKT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AKT3 Mouse Monoclonal Antibody [Clone ID: OTI9B2] (M00520-1)
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No publications found for M00520-1
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