Anti-ILK/Integrin Linked Ilk Rabbit Monoclonal Antibody

Western blot analysis of ILK/Integrin Linked using anti-ILK/Integrin Linked antibody (M02932).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human U20S whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: Rat spleen tissue lysates,
Lane 6: Rat PC-12 whole cell lysates,
Lane 7: Mouse spleen tissue lysates,
Lane 8: Mouse heart tissue lysates,
Lane 9: Rabbit-IgG lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ILK/Integrin Linked antigen affinity purified monoclonal antibody (M02932) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ILK/Integrin Linked at approximately 51 kDa. The expected band size for ILK/Integrin Linked is at 51 kDa.

Western blot analysis of ILK using anti-ILK antibody (M02932).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U2OS whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat Kidney tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ILK antigen affinity purified monoclonal antibody (M02932) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ILK at approximately 51 kDa. The expected band size for ILK is at 51 kDa.

Immunohistochemical analysis of paraffin-embedded human stomach, using ILK Antibody.

Immunofluorescent analysis of 293 cells, using ILK Antibody.