Product Info Summary
SKU: | A00763 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-AMH Antibody Picoband™
SKU/Catalog Number
A00763
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-AMH Antibody Picoband™ catalog # A00763. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AMH Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00763)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human AMH recombinant protein (Position: A75-E141). Human AMH shares 66.7% amino acid (aa) sequence identity with both mouse and rat AMH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00763 is reactive to AMH in Human
Applications
A00763 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
65 kDa
Calculated molecular weight
59.195kDa
Background of MIS/AMH
Anti-Müllerian hormone (AMH), also known as MIF or MIS, is a protein that in humans is encoded by the AMH gene. It is a hormone that inhibits the development of the Müllerian ducts (paramesonephric ducts) in the male embryo. Expression of AMH is activated by SOX9 in the male Sertoli cells and causes the irreversible regression of the Müllerian ducts. Because AMH expression is critical to sex differentiation at a specific time during fetal development, it appears to be tightly regulated by SF1, GATA factors, DAX1 and FSH. This protein also plays a role in Leydig cell differentiation and function and follicular development in adult females. Mutations in this gene result in persistent Mullerian duct syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of AMH using anti-AMH antibody (A00763).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: 293T whole Cell lysates,
Lane 2: COLO320 whole Cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMH antigen affinity purified polyclonal antibody (Catalog # A00763) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMH4 at approximately 65KD. The expected band size for AMH is at 59KD.
Protein Target Info & Infographic
Gene/Protein Information For AMH (Source: Uniprot.org, NCBI)
Gene Name
AMH
Full Name
Muellerian-inhibiting factor
Weight
59.195kDa
Superfamily
TGF-beta family
Alternative Names
AMH; MIF; MIS; Muellerian hormone; muellerian-inhibiting factor; muellerian-inhibiting substance; Mullerian hormone; Mullerian inhibiting factor; Mullerian inhibiting substance AMH MIF, MIS anti-Mullerian hormone muellerian-inhibiting factor|Mullerian inhibiting factor|Mullerian inhibiting substance|anti-Muellerian hormone|muellerian-inhibiting substance
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AMH, check out the AMH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AMH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AMH Antibody Picoband™ (A00763)
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No publications found for A00763
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-AMH Antibody Picoband™
Question
I see that the anti-AMH antibody A00763 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-05-06
Question
We are interested in using your anti-AMH antibody for negative regulation of ovarian follicle development studies. Has this antibody been tested with western blotting on colo320 whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
We appreciate your inquiry. This A00763 anti-AMH antibody is validated on 293t whole cell lysates, colo320 whole cell lysates. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-28
Question
I was wanting to use to test anti-AMH antibody A00763 on human brain for research purposes, then I may be interested in using anti-AMH antibody A00763 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
The products we sell, including anti-AMH antibody A00763, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-17
Question
Does A00763 anti-AMH antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
As indicated on the product datasheet, A00763 anti-AMH antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-22
Question
Will anti-AMH antibody A00763 work for WB with brain?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
According to the expression profile of brain, AMH is highly expressed in brain. So, it is likely that anti-AMH antibody A00763 will work for WB with brain.
Boster Scientific Support
Answered: 2019-06-25
Question
We are currently using anti-AMH antibody A00763 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
The anti-AMH antibody (A00763) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-20
Question
We have observed staining in human cerebellar hemisphere. Are there any suggestions? Is anti-AMH antibody supposed to stain cerebellar hemisphere positively?
Verified Customer
Verified customer
Asked: 2019-01-18
Answer
Based on literature cerebellar hemisphere does express AMH. Based on Uniprot.org, AMH is expressed in cerebellar hemisphere, brain, among other tissues. Regarding which tissues have AMH expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-01-18
Question
Here is the WB image, lot number and protocol we used for brain using anti-AMH antibody A00763. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-10-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-23
Question
Is a blocking peptide available for product anti-AMH antibody (A00763)?
Verified Customer
Verified customer
Asked: 2017-06-28
Answer
We do provide the blocking peptide for product anti-AMH antibody (A00763). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-06-28
Question
My question regarding product A00763, anti-AMH antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Moore
Verified customer
Asked: 2016-11-23
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00763 anti-AMH antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-11-23
Question
My team were well pleased with the WB result of your anti-AMH antibody. However we have been able to see positive staining in cerebellar hemisphere secreted. using this antibody. Is that expected? Could you tell me where is AMH supposed to be expressed?
M. Miller
Verified customer
Asked: 2015-11-12
Answer
Based on literature, cerebellar hemisphere does express AMH. Generally AMH expresses in secreted. Regarding which tissues have AMH expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2015-11-12
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-AMH antibody A00763. Let me know if you need anything else.
J. Johnson
Verified customer
Asked: 2015-07-13
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-07-13
Question
Is there a BSA free version of anti-AMH antibody A00763 available?
J. Carter
Verified customer
Asked: 2015-01-23
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-AMH antibody A00763 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-01-23
Question
I was wanting to use your anti-AMH antibody for WB for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
B. Huang
Verified customer
Asked: 2014-03-24
Answer
It shows on the product datasheet, A00763 anti-AMH antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-03-24
Question
Is this A00763 anti-AMH antibody reactive to the isotypes of AMH?
Z. Yang
Verified customer
Asked: 2013-05-09
Answer
The immunogen of A00763 anti-AMH antibody is E.coli-derived human AMH recombinant protein (Position: A75-E141). Human AMH shares 66.7% amino acid (aa) sequence identity with both mouse and rat AMH. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-05-09