Anti-LIF Antibody Picoband™

Boster Bio Anti-LIF Antibody Picoband™ catalog # A01400. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 3 publication(s).

Product Info Summary

SKU: A01400
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-LIF Antibody Picoband™

See all Lif products

SKU/Catalog Number

A01400

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-LIF Antibody Picoband™ catalog # A01400. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LIF Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01400)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived mouse LIF recombinant protein (Position: S24-F203).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A01400 is reactive to Lif in Human, Mouse, Rat

Applications

A01400 is guaranteed for ELISA, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For Lif (Source: Uniprot.Org, NCBI)

Uniprot ID

P09056

Gene ID

16878

Gene Name

Lif

Full Name

Leukemia inhibitory factor

Weight

22.287kDa

Superfamily

LIF/OSM family

Alternative Names

CDF; D Factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDA; HILDAcholinergic differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor); leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI Lif|leukemia inhibitory factor|leukemia inhibitory factor|d factor|differentiation-stimulating factor|myeloid leukaemia inhibitory factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Lif, check out the Lif Infographic

Lif infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Lif: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A01400 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression

MicroRNA-223-3p suppresses leukemia inhibitory factor expression and pinopodes formation during embryo implantation in mice

Hu Cp, Feng Jt, Tang Yl, Zhu Jq, Lin Mj, Yu Me. Mediators Inflamm. 2006;2006(5):84829. Lif Upregulates Expression Of Nk-1R In Nhbe Cells.

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16 Customer Q&As for Anti-LIF Antibody Picoband™

Question

Can you help my question with product A01400, anti-LIF antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-03-02

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01400 anti-LIF antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-03-02

Question

We are currently using anti-LIF antibody A01400 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?

D. Williams

Verified customer

Asked: 2020-02-10

Answer

The anti-LIF antibody (A01400) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-10

Question

I see that the anti-LIF antibody A01400 works with ELISA, what is the protocol used to produce the result images on the product page?

B. Edwards

Verified customer

Asked: 2019-09-11

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-11

Question

We have tried in the past anti-LIF antibody for WB on colon in a previous project. I am using mouse, and I plan to use the antibody for ELISA next. Our lab want to know about examining colon as well as left coronary artery in our next experiment. Could give a recommendation on which antibody would work the best for ELISA?

Verified Customer

Verified customer

Asked: 2019-08-06

Answer

I have checked the website and datasheets of our anti-LIF antibody and it appears that A01400 has been tested on mouse in both WB and ELISA. Thus A01400 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-08-06

Question

My boss were well pleased with the WB result of your anti-LIF antibody. However we have seen positive staining in left coronary artery secreted. using this antibody. Is that expected? Could you tell me where is LIF supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-07-11

Answer

From what I have seen in literature, left coronary artery does express LIF. Generally LIF expresses in secreted. Regarding which tissues have LIF expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Embryonic stem cell, Pubmed ID: 15146197

Boster Scientific Support

Answered: 2019-07-11

Question

I would like to test anti-LIF antibody A01400 on rat embryonic stem cell for research purposes, then I may be interested in using anti-LIF antibody A01400 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-06-27

Answer

The products we sell, including anti-LIF antibody A01400, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-06-27

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic stem cell using anti-LIF antibody A01400. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-06-19

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-19

Question

We want using your anti-LIF antibody for negative regulation of erk1 and erk2 cascade studies. Has this antibody been tested with western blotting on kidney tissue? We would like to see some validation images before ordering.

E. Patel

Verified customer

Asked: 2018-11-29

Answer

We appreciate your inquiry. This A01400 anti-LIF antibody is validated on rat heart tissue, brain tissue, liver tissue, kidney tissue, human placenta tissue, jurkat cell lysates, mouse liver tissue. It is guaranteed to work for ELISA, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-11-29

Question

Is this A01400 anti-LIF antibody reactive to the isotypes of LIF?

Verified Customer

Verified customer

Asked: 2018-07-24

Answer

The immunogen of A01400 anti-LIF antibody is E. coli-derived mouse LIF recombinant protein (Position: S24-F203). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-07-24

Question

See below the WB image, lot number and protocol we used for embryonic stem cell using anti-LIF antibody A01400. Please let me know if you require anything else.

C. Banerjee

Verified customer

Asked: 2017-08-21

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-08-21

Question

Will A01400 anti-LIF antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

N. Jackson

Verified customer

Asked: 2016-03-31

Answer

As indicated on the product datasheet, A01400 anti-LIF antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2016-03-31

Question

Is a blocking peptide available for product anti-LIF antibody (A01400)?

Z. Johnson

Verified customer

Asked: 2016-02-15

Answer

We do provide the blocking peptide for product anti-LIF antibody (A01400). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2016-02-15

Question

Would anti-LIF antibody A01400 work for ELISA with embryonic stem cell?

N. Carter

Verified customer

Asked: 2015-02-11

Answer

According to the expression profile of embryonic stem cell, LIF is highly expressed in embryonic stem cell. So, it is likely that anti-LIF antibody A01400 will work for ELISA with embryonic stem cell.

Boster Scientific Support

Answered: 2015-02-11

Question

We have seen staining in rat embryonic stem cell. Are there any suggestions? Is anti-LIF antibody supposed to stain embryonic stem cell positively?

D. Parker

Verified customer

Asked: 2015-02-02

Answer

Based on literature embryonic stem cell does express LIF. Based on Uniprot.org, LIF is expressed in left coronary artery, embryonic stem cell, colon, among other tissues. Regarding which tissues have LIF expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Embryonic stem cell, Pubmed ID: 15146197

Boster Scientific Support

Answered: 2015-02-02

Question

I was wanting to use your anti-LIF antibody for ELISA for rat embryonic stem cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat embryonic stem cell identification?

K. Carter

Verified customer

Asked: 2013-11-01

Answer

As indicated on the product datasheet, A01400 anti-LIF antibody has been tested for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat embryonic stem cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-11-01

Question

Is there a BSA free version of anti-LIF antibody A01400 available?

D. Miller

Verified customer

Asked: 2013-01-11

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-LIF antibody A01400 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-01-11

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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Sample Inquiry

In stock
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A01400
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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