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Product Info Summary
| SKU: | EK2104 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human QPCT ELISA Kit PicoKine®
SKU/Catalog Number
EK2104
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human QPCT ELISA Kit PicoKine® (96 Tests). Quantitate Human QPCT in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human QPCT ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2104)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: V29-L361
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is monoclonal antibody from goat.
Sensitivity
<50 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2104 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2104 is reactive to QPCT in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK2104 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of QPCT
Glutaminyl-peptide cyclotransferase is an enzyme that in humans is encoded by the QPCT gene. It is mapped to 2p22.2. This gene encodes human pituitary glutaminyl cyclase, which is responsible for the presence of pyroglutamyl residues in many neuroendocrine peptides. The amino acid sequence of this enzyme is 86% identical to that of bovine glutaminyl cyclase.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2104-CAP | Anti-Human QPCT Pre-coated 96-well strip microplate | 1 |
| EK2104-ST | Human QPCT Standard | 2 vials, 10 ng/tube |
| EK2104-DA | Human QPCT Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.023 | 0.168 | 0.268 | 0.472 | 0.781 | 1.242 | 1.911 | 2.306 |
Data Example Images
Click image to see more details
Human QPCT PicoKine ELISA Kit
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human QPCT ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 301 | 1170 | 4798 | 307 | 1195 | 4812 |
| Standard deviation | 15.65 | 62.01 | 259.09 | 18.73 | 70.51 | 327.22 |
| CV (%) | 5.2% | 5.3% | 5.4% | 6.1% | 5.9% | 6.8% |
Specific Publications For Human QPCT ELISA Kit PicoKine® (EK2104)
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Human QPCT ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-05-31
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/elisa-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/elisa-data-analysis-instructions
Boster Scientific Support
Answered: 2020-05-31
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-05-27
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-05-27
Question
Q: which procedure should I follow in order to thaw whole blood sample for QPCT ELISA after freezing?
P. Phillips
Verified customer
Asked: 2018-12-19
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test QPCT for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-12-19
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-02-09
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-02-09
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
L. Mitchell
Verified customer
Asked: 2018-01-26
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-01-26
Question
Q: is it okay to use citrate plasma as samples in Human QPCT Picokine® ELISA Kit (Catalog # EK2104)?
Verified Customer
Verified customer
Asked: 2017-12-30
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of QPCT causing degradation of its protein structure. QPCT may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the QPCT ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-12-30
Question
Q: if the enzyme conjugated QPCT antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the QPCT antigen?
Verified Customer
Verified customer
Asked: 2017-11-08
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-11-08
Question
Q: how to proceed with the analysis of ELISA data? I have performed QPCT level in serum.
M. Varma
Verified customer
Asked: 2015-12-27
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2015-12-27
