Product Info Summary
| SKU: | EK0764 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
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Product info
Product Name
Human Galectin-3/LGALS3 ELISA Kit PicoKine®
View all Galectin-3 ELISA kits
SKU/Catalog Number
EK0764
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Galectin-3/LGALS3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human LGALS3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Galectin-3/LGALS3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0764)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: A2-I250
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0764 is reactive to LGALS3 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0764 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Galectin-3
Galectin-3 (GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, LGALS3, located on chromosome 14, locus q21–q22. It is expressed in the nucleus, cytoplasm,mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0764-CAP | Anti-Human LGALS3 Pre-coated 96-well strip microplate | 1 |
| EK0764-ST | Human LGALS3 Standard | 2 vials, 10 ng/tube |
| EK0764-DA | Human LGALS3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.029 | 0.071 | 0.151 | 0.265 | 0.507 | 0.837 | 1.315 | 1.810 |
Data Example Images
Click image to see more details
Human Galectin-3/LGALS3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Galectin-3, Dilution ratio of 1:5, concentration in serum is 4-10ng/ml.
Some articles we found to cite concentrations of Galectin-3 in samples: 25604929 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Galectin-3/LGALS3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 212 | 1876 | 4230 | 205 | 1951 | 4307 |
| Standard deviation | 16.74 | 76.91 | 334.17 | 19.88 | 118.70 | 374.7 |
| CV (%) | 7.9% | 4.1% | 7.9% | 9.7% | 5.9% | 8.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Galectin-3 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 212 | 196 | 200 | 216 | 206 | 8.24 | 4% |
| Sample 2 | 1876 | 2001 | 1983 | 1897 | 1939 | 53.64 | 2.7% |
| Sample 3 | 4230 | 4475 | 3962 | 4238 | 4226 | 181.56 | 4.2% |
Protein Target Info & Infographic
Gene/Protein Information For LGALS3 (Source: Uniprot.Org, NCBI)
Uniprot ID
P17931
Gene ID
3958
Gene Name
LGALS3
Full Name
Galectin-3
Weight
26.152kDa
Alternative Names
AGE-R3; Carbohydrate-binding protein 35; CBP 35,35 kDa lectin; CBP35; GAL3; Gal-3; galactin-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBPCBP35; Galectin3; Galectin-3; GALIG; IgE-binding protein; L29; L31; L-31; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS2; LGALS3; Mac-2 antigen; Mac-2; MAC2GAL3 LGALS3 CBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2 galectin 3 galectin-3|35 kDa lectin|IgE-binding protein|MAC-2 antigen|advanced glycation end-product receptor 3|carbohydrate-binding protein 35|epididymis secretory sperm binding protein|galactose-specific lectin 3|laminin-binding protein|lectin L-29|lectin, galactoside-binding, soluble, 3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on LGALS3, check out the LGALS3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LGALS3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Human Galectin-3/LGALS3 ELISA Kit PicoKine® (EK0764)
EK0764 has been cited in 9 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The Interplay between Myocardial Fibrosis, Strain Imaging and Collagen Biomarkers in Adults with Repaired Tetralogy of Fallot
Maternal serum galectin-1 and galectin-3 levels in pregnancies complicated with preterm prelabor rupture of membranes
Galectin-3 mediates pulmonary vascular remodeling in hypoxia-induced pulmonary arterial hypertension
Comparison between galectin-3 and YKL-40 levels for the assessment of liver fibrosis in cirrhotic patients
Galectin-1 and Galectin-3 Levels in Patients with Schizophrenia and their Unaffected Siblings
Dadarlat-Pop A,Pop D,Procopciuc L,Sitar-Taut A,Zdrenghea D,Bodizs G,Tomoaia R,Gurzau D,Fringu F,Susca-Hojda S,Buzoianu AD.Leptin, Galectin-3 and Angiotensin II Type 1 Receptor Polymorphism in Overweight and Obese Patients with Heart Failure – Role and Functional Interplay.Int J Gen Med.2021;14:1727-1737 https://doi.org/10.2147/IJGM.S301285
Species: Human
EK0764 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Clinical significance of galectin-3 expression in malformed hepatic venous tissue
Prospective Validation of a Screening Biomarker Approach Combining Amino-Terminal Pro-Brain Natriuretic Peptide With Galectin-3 Predicts Death and Cardiovascular Events in Asymptomatic Hemodialysis Patients
Predictive Value for Galectin 3 and Cardiotrophin 1 in Hemodialysis Patients
Recommended Resources
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- Boster's Pathways Library
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
9 Customer Q&As for Human Galectin-3/LGALS3 ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated Galectin-3 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Galectin-3 antigen?
J. Wang
Verified customer
Asked: 2020-11-21
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-11-21
Question
Q: can I use citrate plasma as samples in Human Galectin-3 Picokine® ELISA Kit (Catalog # EK0764)?
Verified Customer
Verified customer
Asked: 2020-07-12
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Galectin-3 causing degradation of its protein structure. Galectin-3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Galectin-3 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-07-12
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Galectin-3 using the EK0764 Human Galectin-3 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-06-07
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2020-06-07
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Galectin-3 in Human plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-10-19
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Galectin-3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-10-19
Question
Q: how to thaw whole blood sample for Galectin-3 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-06-18
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Galectin-3 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-06-18
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
W. Chakrabarti
Verified customer
Asked: 2018-03-27
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-03-27
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
E. Anderson
Verified customer
Asked: 2018-01-29
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-01-29
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
H. Nelson
Verified customer
Asked: 2017-04-17
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-04-17
Question
Q: What is the optimal O.D. value for Galectin-3 ELISA kit? I performed your Galectin-3 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Galectin-3 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2016-11-24
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Galectin-3 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Galectin-3 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2016-11-24
