Overview
| Product Name |
Human Galectin-3/LGALS3 ELISA Kit PicoKine™
See all Galectin-3 primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
EK0764 |
| Storage & Handling |
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description |
Detect Human Galectin 3/LGALS3 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Galectin 3/LGALS3 tissue specificity: A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes. . |
| Cite This Product |
Human Galectin-3/LGALS3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0764)
|
Product Specs
| Reactivity/Species |
Human |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity |
<10pg/ml |
| Assay Range |
156pg/ml-10000pg/ml |
| Immunogen/Standard |
Expression system for standard: E.coli; Immunogen sequence: A2-I250 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96wells/kit, with removable strips. |
Gene/Protein Basic Information For LGALS3 (Source: Uniprot.org, NCBI)
| Uniprot Id | P17931 |
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| NCBI Gene Id | 3958 |
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| Species Of This Entry | Human |
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| Gene Name | LGALS3 |
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| Protein Name | Galectin-3 |
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| Alternative Names | Galectin-3|AGE-R3; Carbohydrate-binding protein 35; CBP 35,35 kDa lectin; CBP35; GAL3; Gal-3; galactin-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBPCBP35; Galectin3; Galectin-3; GALIG; IgE-binding protein; L29; L31; L-31; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS2; LGALS3; Mac-2 antigen; Mac-2; MAC2GAL3 |
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| Molecular Weight | 26152 |
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| Post Tranlational Modifications | Phosphorylation; Methylation; Cleavage; Glycosylation; Demethylation; Oxidation |
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| Subcellular Localization | Cytoplasm. Nucleus. Secreted. Secreted by a non-classical secretory pathway and associates with the cell surface. |
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| Gene Location | 14q22.3, on Chromasome 14, gene sequence: NC_000014.9 (55129252..55145430) |
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*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For LGALS3 (Source: Uniprot.org, NCBI)
| Protein Function | Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. |
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| Tissue Specificity | A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes. |
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| Interacting Proteins | ALCAM; CD6; CHI3L1; GOLGA2; IL13RA2; MICA; MMP7; NCR3; PDCD6IP; PPIG; PRR13; SS18L1 |
|---|
| Related Diseases | Neoplasms; Malignant Neoplasms; Inflammation; Tissue Adhesions; Carcinoma; Thyroid Neoplasm; Fibrosis; Adenoma; Malignant Paraganglionic Neoplasm; Malignant Neoplasm Of Thyroid; Carcinoma, Papillary; Atherosclerosis; Neoplasm Metastasis; Pituitary Neoplasms; Cell Transformation, Neoplastic; Tumor Angiogenesis; Pituitary Diseases; Papillary Thyroid Carcinoma; Thyroid Nodule; Tumor Progression |
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| Related Pathways | Pathogenesis; Immune Response; Cell Proliferation; Cell Adhesion; Dna Methylation; Methylation; Cell Growth; Angiogenesis; Inflammatory Response; Cell Migration; Macrophage Activation; Transport; Cell Death; Cell Activation; Regeneration; Phagocytosis; Cell Cycle; Localization; Cytokine Production; Secretion |
|---|
| Background | Galectin-3(GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, LGALS3, located on chromosome 14, locus q21–q22. It is expressed in the nucleus, cytoplasm,mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes. |
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| Scientific References | PMID: 2261464 by Robertson M.W., et al. Human IgE-binding protein: a soluble lectin exhibiting a highly conserved interspecies sequence and differential recognition of IgE glycoforms.
PMID: 2402511 by Cherayil B., et al. Molecular cloning of a human macrophage lectin specific for galactose. |
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Kit Components And Assay QC Data Details Of Human Galectin-3/LGALS3 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0764-CAP | 96-well plate precoated with anti-Human Galectin-3 antibody | 1 |
| EK0764-ST | lyophilized recombinant Human Galectin-3 standard | 10ng/tube |
| EK0764-DA | biotinylated anti-Human Galectin-3 antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0764 Human Galectin-3/LGALS3 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.029 | 0.151 | 0.265 | 0.507 | 0.837 | 1.315 | 1.810 | 2.149 |
We measured random samples of Human Galectin-3/LGALS3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 212 | 1876 | 4230 | 205 | 1951 | 4307 |
| Standard deviation | 16.74 | 76.91 | 334.17 | 19.88 | 118.70 | 374.7 |
| CV(%) | 7.9% | 4.1% | 7.9% | 9.7% | 5.9% | 8.7% |
We measured the performance consistency of Human Galectin-3/LGALS3 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 212 | 196 | 200 | 216 | 206 | 8.24 | 4% |
| Sample 2 | 1876 | 2001 | 1983 | 1897 | 1939 | 53.64 | 2.7% |
| Sample 3 | 4230 | 4475 | 3962 | 4238 | 4226 | 181.56 | 4.2% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
See all Galectin-3 primary antibodies,
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Total number of citations: 5
| Predictive Value for Galectin 3 and Cardiotrophin 1 in Hemodialysis Patients |
PubMed ID 26787685 |
| Prospective Validation of a Screening Biomarker Approach Combining Amino-Terminal Pro-Brain Natriuretic Peptide With Galectin-3 Predicts Death and Cardiovascular Events in Asymptomatic Hemodialysis Patients |
PubMed ID 28974104 |
| Clinical significance of galectin-3 expression in malformed hepatic venous tissue |
PubMed ID 30778007 |
| Maternal serum galectin-1 and galectin-3 levels in pregnancies complicated with preterm prelabor rupture of membranes |
PubMed ID 31242786 |
| The roles of galectin-3 and galectin-4 in the idiopatic Parkinson disease and its progression |
PubMed ID 31147178 |
