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Product Info Summary
| SKU: | PB9279 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Galectin 3/LGALS3 Antibody Picoband®
SKU/Catalog Number
PB9279
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Galectin 3/LGALS3 Antibody Picoband® catalog # PB9279. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Galectin 3/LGALS3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9279)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Galectin 3 recombinant protein (Position: K153-I264). Mouse Galectin 3 shares 88% and 91% amino acid (aa) sequence identity with human and rat Galectin 3, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9279 is reactive to Lgals3 in Mouse, Rat
Observed Molecular Weight
29 kDa
Calculated molecular weight
27.5 kDa
Background of Lgals3
Galectin-3 (GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, it located on chromosome 14, locus q21–q22. It is expressed in the nucleus, cytoplasm,mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9279 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat
Positive Control
WB: rat lung tissue, rat stomach tissue, mouse lung tissue, mouse stomach tissue
IHC: mouse lung tissue, rat lung tissue
Validation Images & Assay Conditions
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Western blot analysis of Galectin 3/LGALS3 using anti-Galectin 3/LGALS3 antibody (PB9279).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: rat stomach tissue lysates,
Lane 3: mouse lung tissue lysates,
Lane 4: mouse stomach tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 3/LGALS3 antigen affinity purified polyclonal antibody (Catalog # PB9279) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 3/LGALS3 at approximately 29 kDa. The expected band size for Galectin 3/LGALS3 is at 29 kDa.
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IHC analysis of Galectin 3/LGALS3 using anti-Galectin 3/LGALS3 antibody (PB9279).
Galectin 3/LGALS3 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Galectin 3/LGALS3 Antibody (PB9279) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of Galectin 3/LGALS3 using anti-Galectin 3/LGALS3 antibody (PB9279).
Galectin 3/LGALS3 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Galectin 3/LGALS3 Antibody (PB9279) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Galectin 3/LGALS3 Antibody Picoband® (PB9279)
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1 Customer Q&As for Anti-Galectin 3/LGALS3 Antibody Picoband®
Question
We are currently using anti-Galectin 3/LGALS3 antibody PB9279 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
The anti-Galectin 3/LGALS3 antibody (PB9279) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-12
