Anti-Annexin V/ANXA5 Antibody Picoband®

Product Name

Anti-Annexin V/ANXA5 Antibody Picoband®

View all ANXA5 Antibodies

SKU/Catalog Number

PA1008
BA0644 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

Annexin A5 (Annexin V; ANXA5) is a Ca2+-dependent phospholipid-binding protein widely used as a readout of phosphatidylserine exposure during apoptosis/cell death biology. Assay context: it is tested in IHC/ICC/WB and reacts with Human/Mouse/Rat, aligning with tissue/cell staining and lysate validation workflows (research use). In panels, ANXA5 biology is often interpreted alongside inflammatory cues such as IL-10 (resolution signaling) and chemokines like CCL4 when apoptosis occurs in inflammatory microenvironments (putative); tissue-level interpretation can be paired with histologic review conventions in pathology review.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Annexin V/ANXA5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1008)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Annexin V, identical to the related rat and mouse sequences.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1008 is reactive to ANXA5 in Human, Mouse, Rat

Observed Molecular Weight

36 kDa

Calculated molecular weight

35.9 kDa

Background of ANXA5

Annexin V also know as endonnexin II (ENX2),or placental protein 4 (PP4). Endonexin II is a member of the family of Ca (2+)-dependent phospholipid binding proteins, known as annexins. It bind to the phospholipids that are preferentially located on the cytosolic face of the plasma membrane. It has a relative molecular weight of about 35,000. The gene lies on mouse chromosome 3 in close linkage with the fibroblast growth factor 2 (basic) gene and is syntenic with other genes known to have orthologous counterparts on human chromosome 4q. The PP4 cDNA encoded a protein of 320 amino acid residues. A single mRNA, approximately 1.6 kb long, was found to be expressed in human cell lines and placenta. PP4 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1008 is guaranteed for IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Positive Control

WB: Rat Brain Tissue, Rat Skeletal Muscle Tissue, Rat Ovary Tissue, Rat Lung Tissue, MCF-7 Whole Cell, SMMC Whole Cell, A549 Whole Cell, JURKAT Whole Cell, SGC Whole Cell, HT1080 Whole Cell
IHC: Rat Liver Tissue
ICC: HCT116 Cell, HELA Cell

Validation Images & Assay Conditions

See full target information ANXA5

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