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- Table of Contents
ANXA5 antibody (PA1008) performs well in IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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Anti-Annexin V antibody, PA1008, ICC
ICC: HCT116 Cell
ICC: HCT116 Cell
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Anti-Annexin V/ANXA5 Antibody Picoband®
ANXA5 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, ICC, WB |
Tested Applications
ANXA5 antibody was positively detected by WB in:
Rat Brain Tissue, Rat Skeletal Muscle Tissue, Rat Ovary Tissue, Rat Lung Tissue, MCF-7 Whole Cell, SMMC Whole Cell, A549 Whole Cell, JURKAT Whole Cell, SGC Whole Cell, HT1080 Whole Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ANXA5 antibody was positively detected by IHC in:
Rat Liver Tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ANXA5 antibody was positively detected by ICC in:
HCT116 Cell, HELA Cell
Recommend Dilution
Application
Dilution
Species
Immunocytochemistry
0.5-1μg/ml
Human, -
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Rat, Mouse
Western blot
0.1-0.5μg/ml
Human, Rat, Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Rat
Tested
Tested
Recommend
Human
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Annexin V/ANXA5 Antibody Picoband®
View all ANXA5 Antibodies
View all ANXA5 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Annexin V, identical to the related rat and mouse sequences.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36 kDa
Calculated Molecular Weight
35.9 kDa
Background of ANXA5
Annexin V also know as endonnexin II (ENX2),or placental protein 4 (PP4). Endonexin II is a member of the family of Ca (2+)-dependent phospholipid binding proteins, known as annexins. It bind to the phospholipids that are preferentially located on the cytosolic face of the plasma membrane. It has a relative molecular weight of about 35,000. The gene lies on mouse chromosome 3 in close linkage with the fibroblast growth factor 2 (basic) gene and is syntenic with other genes known to have orthologous counterparts on human chromosome 4q. The PP4 cDNA encoded a protein of 320 amino acid residues. A single mRNA, approximately 1.6 kb long, was found to be expressed in human cell lines and placenta. PP4 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Cite This Product
Anti-Annexin V/ANXA5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1008)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Zebrafish
33%
Low homology
Drosophila
33%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ANXA5 antibody
Immunohistochemistry (IHC) protocol for ANXA5 antibody
Immunocytochemistry (ICC) protocol for ANXA5 antibody
Publications
Application:
Annexin A1, A2, A4 and A5 play important roles in breast cancer, pancreatic cancer and laryngeal carcinoma, alone and/or synergistically
PMID: 30242262
Morphological and Proteomic Analyses Reveal that Unsaturated Guluronate Oligosaccharide Modulates Multiple Functional Pathways in Murine Macrophage RAW264.7 Cells
PMID: 25830683
Xiong Y, Ma Xy, Zhang Z, Shao Zj, Zhang Yy, Zhou Lm. Oncol Lett. 2013 Dec;6(6):1789-1793. Epub 2013 Oct 10. Apoptosis Induced By ??,??-Dimethylacrylshikonin Is Associated With Bcl-2 And Nf-??b In Human Breast Carcinoma Mcf-7 Cells.
PMID: 24260077
Jin H, Liu T, Wang Wx, Xu Jh, Yang Pb, Lu Hx, Sun Qr, Hu Ht. Neurochem Int. 2010 Feb;56(3):417-23. Doi: 10.1016/J.Neuint.2009.11.015. Epub 2009 Nov 24. Protective Effects Of [Gly14]-Humanin On Beta-Amyloid-Induced Pc12 Cell Death By Preventing Mit...
PMID: 19941922
Hu Xj, Dong Ng, Shi Jw, Deng C, Li Hd, Lu Cf. J Biomed Mater Res B Appl Biomater. 2014 Feb;102(2):322-36. Doi: 10.1002/Jbm.B.33010. Epub 2013 Sep 20. Evaluation Of A Novel Tetra-Functional Branched Poly(Ethylene Glycol) Crosslinker For Manufacture...
PMID: 24115395
Ji N, Li J, Wei Z, Kong F, Jin H, Chen X, Li Y, Deng Y. Biomed Res Int. 2015;2015:308475. Doi: 10.1155/2015/308475. Epub 2015 Mar 19. Effect Of Celastrol On Growth Inhibition Of Prostate Cancer Cells Through The Regulation Of Herg Channel In Vitro.
PMID: 25866772
Sun X, Kang H, Yao Y, Chen H, Sun L, An W, Jiang E, Wang S, Hu X. Anticancer Drugs. 2015 Jul;26(6):641-8. Doi: 10.1097/Cad.0000000000000229. Dehydrocostus Lactone Suppressed The Proliferation, Migration, And Invasion Of Colorectal Carcinoma Throug...
PMID: 25850883
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Product Promise
We promise Anti-Annexin V/ANXA5 Antibody Picoband® performs excellently in relevant applications for IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA1008
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026