Anti-Annexin V/ANXA5 Antibody

Rabbit Polyclonal Annexin V/ANXA5 Antibody. Validated in IHC, ICC, WB and tested in Human, Mouse, Rat.

SKU PA1008
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications IHC, ICC, WB


Product Name Anti-Annexin V/ANXA5 Antibody
See all ANXA5 primary antibodies, ELISA kits and proteins
SKU/Catalog Number PA1008
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit Polyclonal Annexin V/ANXA5 Antibody. Validated in IHC, ICC, WB and tested in Human, Mouse, Rat. Size: 100μg/vial.
Cite This Product Anti-Annexin V/ANXA5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1008)
Specificity Anti-Annexin V/ANXA5 Antibody (PA1008) reacts with Human, Mouse, Rat ANXA5, in native form and recombinant. Superfamily members of ANXA5 are not reactive to PA1008.
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Form Lyophilized
Immunogen Anti-Annexin V/ANXA5 Antibody (PA1008) was raised against A synthetic peptide corresponding to a sequence in the middle region of human Annexin V(88-102aa SRLYDAYELKHALKG), identical to the related rat and mouse sequences..
Reactivity Human, Mouse, Rat

Assay Details

Our Boster Quality Guarantee for Anti-Annexin V/ANXA5 Antibody covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Boster's Compatible Products

The following reagents are used to generate the images below for Anti-Annexin V/ANXA5 Antibody (PA1008).

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.

Images And Assay Conditions

/p/a/pa1008 1_1.jpg

Anti-Annexin V antibody, PA1008, Western blotting
All lanes: Anti Annexin V (PA1008) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 3: Rat Ovary Tissue Lysate at 50ug
Lane 4: Rat Lung Tissue Lysate at 50ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Lane 8: JURKAT Whole Cell Lysate at 40ug
Lane 9: SGC Whole Cell Lysate at 40ug
Lane 10: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Observed bind size: 36KD

/antibody/pa1008 2 IHC anti annexin v antibody.jpg

Anti-Annexin V antibody, PA1008, IHC(P)
IHC(P): Rat Liver Tissue

/antibody/pa1008 3 IF anti annexin v antibody.jpg

Anti-Annexin V antibody, PA1008, ICC
ICC: HCT116 Cell

/antibody/pa1008 4 IF anti annexin v antibody.jpg

Anti-Annexin V antibody, PA1008, ICC

Target Info

Protein Target Info (Source:

Uniprot Id P08758
Gene Name ANXA5
Protein Name Annexin A5
Alternative Names Annexin A5;Anchorin CII;Annexin V;Annexin-5;Calphobindin I;CBP-I;Endonexin II;Lipocortin V;Placental anticoagulant protein 4;PP4;Placental anticoagulant protein I;PAP-I;Thromboplastin inhibitor;Vascular anticoagulant-alpha;VAC-alpha;ANXA5;ANX5, ENX2, PP4;Show more
Molecular Weight 35937 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.


Protein Function This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Research Areas Cell Biology, Cell Death, Invasion/Microenvironment

*You can search these to find other products in these research areas.
Background Annexin V also know as endonnexin II(ENX2),or placental protein 4(PP4). Endonexin II is a member of the family of Ca(2+)-dependent phospholipid binding proteins, known as annexins. It bind to the phospholipids that are preferentially located on the cytosolic face of the plasma membrane. It has a relative molecular weight of about 35,000. The gene lies on mouse chromosome 3 in close linkage with the fibroblast growth factor 2(basic) gene and is syntenic with other genes known to have orthologous counterparts on human chromosome 4q. The PP4 cDNA encoded a protein of 320 amino acid residues. A single mRNA, approximately 1.6 kb long, was found to be expressed in human cell lines and placenta. PP4 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Publishing with Anti-Annexin V/ANXA5 Antibody (PA1008)? Please let us know so we can cite the reference in this product datasheet. Email us at

Order Product (PA1008)


Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1054 $280
100ug+Free Biotin Secondary BA1003 $280

USD $280

Ships next business day.


Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

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Boster Guarantee

Guaranteed product quality

We promise all of our products perform as described in datasheets.

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Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to annexin v antibody, annexin 5 antibody, annexin a5 antibody, annexin-5 antibody