Anti-Annexin V/ANXA5 Antibody

Annexin V antibody

Boster Bio Anti-Annexin V/ANXA5 Antibody catalog # PA1008. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 7 publication(s).

Product Info Summary

SKU: PA1008
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, ICC, WB

Product Name

Anti-Annexin V/ANXA5 Antibody

View all Annexin V Antibodies

SKU/Catalog Number

PA1008

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Annexin V/ANXA5 Antibody catalog # PA1008. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Annexin V/ANXA5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1008)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Annexin V, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1008 is reactive to ANXA5 in Human, Mouse, Rat

Applications

PA1008 is guaranteed for IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

36 kDa

Calculated molecular weight

35.937kDa

Background of Annexin V

Annexin V also know as endonnexin II (ENX2),or placental protein 4 (PP4). Endonexin II is a member of the family of Ca (2+)-dependent phospholipid binding proteins, known as annexins. It bind to the phospholipids that are preferentially located on the cytosolic face of the plasma membrane. It has a relative molecular weight of about 35,000. The gene lies on mouse chromosome 3 in close linkage with the fibroblast growth factor 2 (basic) gene and is syntenic with other genes known to have orthologous counterparts on human chromosome 4q. The PP4 cDNA encoded a protein of 320 amino acid residues. A single mRNA, approximately 1.6 kb long, was found to be expressed in human cell lines and placenta. PP4 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For ANXA5 (Source: Uniprot.org, NCBI)

Gene Name

ANXA5

Full Name

Annexin A5

Weight

35.937kDa

Superfamily

annexin family

Alternative Names

Anchorin CII; annexin A5; Annexin V; annexin-5; ANX5; ANXA5; ANXV; Calphobindin I; Endonexin II; ENX2CBP-I; Lipocortin V; PAP-I; Placental anticoagulant protein 4; Placental anticoagulant protein I; PP4; Thromboplastin inhibitor; VAC-alpha; Vascular anticoagulant-alpha ANXA5 ANX5, ENX2, HEL-S-7, PP4, RPRGL3 annexin A5 annexin A5|CBP-I|PAP-I|VAC-alpha|anchorin CII|annexin V|annexin-5|calphobindin I|endonexin II|epididymis secretory protein Li 7|lipocortin V|placental anticoagulant protein 4|placental anticoagulant protein I|thromboplastin inhibitor|vascular anticoagulant-alpha

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ANXA5, check out the ANXA5 Infographic

ANXA5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ANXA5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1008 has been cited in 7 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Annexin A1, A2, A4 and A5 play important roles in breast cancer, pancreatic cancer and laryngeal carcinoma, alone and/or synergistically

Morphological and Proteomic Analyses Reveal that Unsaturated Guluronate Oligosaccharide Modulates Multiple Functional Pathways in Murine Macrophage RAW264.7 Cells

Xiong Y, Ma Xy, Zhang Z, Shao Zj, Zhang Yy, Zhou Lm. Oncol Lett. 2013 Dec;6(6):1789-1793. Epub 2013 Oct 10. Apoptosis Induced By ??,??-Dimethylacrylshikonin Is Associated With Bcl-2 And Nf-??b In Human Breast Carcinoma Mcf-7 Cells.

Jin H, Liu T, Wang Wx, Xu Jh, Yang Pb, Lu Hx, Sun Qr, Hu Ht. Neurochem Int. 2010 Feb;56(3):417-23. Doi: 10.1016/J.Neuint.2009.11.015. Epub 2009 Nov 24. Protective Effects Of [Gly14]-Humanin On Beta-Amyloid-Induced Pc12 Cell Death By Preventing Mit...

Hu Xj, Dong Ng, Shi Jw, Deng C, Li Hd, Lu Cf. J Biomed Mater Res B Appl Biomater. 2014 Feb;102(2):322-36. Doi: 10.1002/Jbm.B.33010. Epub 2013 Sep 20. Evaluation Of A Novel Tetra-Functional Branched Poly(Ethylene Glycol) Crosslinker For Manufacture...

Ji N, Li J, Wei Z, Kong F, Jin H, Chen X, Li Y, Deng Y. Biomed Res Int. 2015;2015:308475. Doi: 10.1155/2015/308475. Epub 2015 Mar 19. Effect Of Celastrol On Growth Inhibition Of Prostate Cancer Cells Through The Regulation Of Herg Channel In Vitro.

Sun X, Kang H, Yao Y, Chen H, Sun L, An W, Jiang E, Wang S, Hu X. Anticancer Drugs. 2015 Jul;26(6):641-8. Doi: 10.1097/Cad.0000000000000229. Dehydrocostus Lactone Suppressed The Proliferation, Migration, And Invasion Of Colorectal Carcinoma Throug...

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5 Customer Q&As for Anti-Annexin V/ANXA5 Antibody

Question

Can PA1008 be used in immunoprecipitation?

Verified customer

Asked: 2021-01-29

Answer

Unfortunately, the Anti-Annexin V/ANXA5 Antibody (PA1008) hasn't been validated in immunoprecipitation. We suggest to run a pilot test to see if it can work for immunoprecipitation.

Boster Scientific Support

Answered: 2021-01-31

Question

I see that the anti-Annexin V/ANXA5 antibody PA1008 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-11-05

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-11-05

Question

My question regards to test anti-Annexin V/ANXA5 antibody PA1008 on mouse ovary skin for research purposes, then I may be interested in using anti-Annexin V/ANXA5 antibody PA1008 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

P. Mitchell

Verified customer

Asked: 2019-02-25

Answer

The products we sell, including anti-Annexin V/ANXA5 antibody PA1008, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-02-25

Question

Would anti-Annexin V/ANXA5 antibody PA1008 work for IHC with ovary skin?

G. Mangal

Verified customer

Asked: 2018-03-01

Answer

According to the expression profile of ovary skin, ANXA5 is highly expressed in ovary skin. So, it is likely that anti-Annexin V/ANXA5 antibody PA1008 will work for IHC with ovary skin.

Boster Scientific Support

Answered: 2018-03-01

Question

I have a question about product PA1008, anti-Annexin V/ANXA5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

L. Moore

Verified customer

Asked: 2016-09-01

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1008 anti-Annexin V/ANXA5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-09-01

Order DetailsPrice
PA1008

100μg

$370
PA1008-10ug

10μg sample (liquid)

$99
PA1008-Biotin

100 μg Biotin conjugated

$570
PA1008-Cy3

100 μg Cy3 conjugated

$570
PA1008-Dylight488

100 μg Dylight488 conjugated

$570
PA1008-Dylight550

100 μg Dylight550 conjugated

$570
PA1008-Dylight594

100 μg Dylight594 conjugated

$570
PA1008-FITC

100 μg FITC conjugated

$570
PA1008-HRP

100 μg HRP conjugated

$570
PA1008-APC

100 μg APC conjugated

$670
PA1008-PE

100 μg PE conjugated

$670
PA1008-carrier-free

Carrier Free

$370

More conjugates/formats

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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