Human CCL4/MIP-1 Beta ELISA Kit PicoKine®

Product Name

Human CCL4/MIP-1 Beta ELISA Kit PicoKine®

SKU/Catalog Number

EK0450

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

CCL4 (MIP-1β) is a CC chemokine with specificity for CCR5; the page notes chemoattraction of NK cells/monocytes and highlights CCL4 as a major HIV-suppressive factor produced by CD8+ T cells. Assay context: quantitative ELISA with sensitivity <1 pg/mL and assay range 7.8–500 pg/mL; validated sample types include cell culture supernatants, serum, and plasma (heparin/EDTA/citrate), supporting high-sensitivity inflammatory chemokine profiling (research use). Often analyzed alongside inflammatory S100 proteins such as S100A12 and macrophage-lineage context markers like CD163 or CSF1R when interpreting myeloid recruitment/activation (putative); multiplexing considerations are commonly discussed under multiplex assay workflows.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CCL4/MIP-1 Beta ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0450)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: A24-N92

Sensitivity

<1 pg/ml

Assay Range

7.8 pg/ml - 500 pg/ml

Standard Dilution Instructions

See datasheet of EK0450 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0450 is reactive to CCL4 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK0450 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CCL4

Chemokine (C-C motif) ligand 4, also known as CCL4, is a protein which in humans is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. the CCL4 gene was assigned to a slightly more distal location: 17q21-q23 rather than 17q11-q21.

Kit Components

Catalog Number	Description	Quantity
EK0450-CAP	Anti-Human CCL4 Pre-coated 96-well strip microplate	1
EK0450-ST	Human CCL4 Standard	2 vials, 10 ng/tube
EK0450-DA	Human CCL4 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
O.D.	0.044	0.141	0.243	0.419	0.719	1.163	1.712	2.022

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect CCL4, Dilution ratio of 1:1, concentration in serum and plasma is 0-300 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CCL4/MIP-1 Beta ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

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