Anti-AP3D1/Ap3 Antibody

Boster Bio Anti-AP3D1/Ap3 Antibody catalog # A07250. Tested in IP, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A07250
Size: 100μg
Reactive Species: Human
Host: Goat
Application: IP, WB

Product Name

Anti-AP3D1/Ap3 Antibody

See all AP3D1 products

SKU/Catalog Number

A07250

Size

100μg

Form

Liquid

Description

Boster Bio Anti-AP3D1/Ap3 Antibody catalog # A07250. Tested in IP, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AP3D1/Ap3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07250)

Host

Goat

Contents

0.02 M Potassium Phosphate
0.15 M Sodium Chloride
pH 7.2
0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Immunogen

This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 686-698 of Human AP3D1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A07250 is reactive to AP3D1 in Human

Applications

A07250 is guaranteed for IP, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2, 000, human, chimpanzee and chicken
Direct ELISA, 1:5, 000 - 1:20, 000, human, chimpanzee and chicken
ImmunoPrecipitation: 1:100, human, chimpanzee and chicken

Validation Images & Assay Conditions

Gene/Protein Information For AP3D1 (Source: Uniprot.Org, NCBI)

Uniprot ID

O14617

Gene ID

8943

Gene Name

AP3D1

Full Name

AP-3 complex subunit delta-1

Weight

130.158kDa

Superfamily

adaptor complexes large subunit family

Alternative Names

Adapter-related protein complex 3 subunit delta-1; adaptin, delta; adaptor-related protein complex 3, delta 1 subunit; ADTD; AP-3 complex delta subunit, partial CDS; AP-3 complex subunit delta; AP-3 complex subunit delta-1; delta adaptin; Delta-adaptin; delta-adaptin, partial CDS; hBLVR; subunit of putative vesicle coat adaptor complex AP-3 AP3D1 ADTD, HPS10, hBLVR adaptor related protein complex 3 subunit delta 1 AP-3 complex subunit delta-1|AP-3 complex delta subunit, partial CDS|adapter-related protein complex 3 subunit delta-1|adaptor related protein complex 3 delta 1 subunit|delta adaptin|subunit of putative vesicle coat adaptor complex AP-3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on AP3D1, check out the AP3D1 Infographic

AP3D1 infographic

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In this infographic you will see the following information for AP3D1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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6 Customer Q&As for Anti-AP3D1/Ap3 Antibody

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for platelet using anti-AP3D1/Ap3 antibody A07250. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-03-24

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-24

Question

Would A07250 anti-AP3D1/Ap3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-22

Answer

You can see on the product datasheet, A07250 anti-AP3D1/Ap3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-22

Question

I am interested in to test anti-AP3D1/Ap3 antibody A07250 on human platelet for research purposes, then I may be interested in using anti-AP3D1/Ap3 antibody A07250 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-08-19

Answer

The products we sell, including anti-AP3D1/Ap3 antibody A07250, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-08-19

Question

Is this A07250 anti-AP3D1/Ap3 antibody reactive to the isotypes of AP3D1?

Verified Customer

Verified customer

Asked: 2019-06-11

Answer

The immunogen of A07250 anti-AP3D1/Ap3 antibody is This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 686-698 of Human AP3D1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-11

Question

I have attached the WB image, lot number and protocol we used for platelet using anti-AP3D1/Ap3 antibody A07250. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-10-26

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-10-26

Question

We are currently using anti-AP3D1/Ap3 antibody A07250 for human tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?

B. Gonzalez

Verified customer

Asked: 2014-08-11

Answer

The anti-AP3D1/Ap3 antibody (A07250) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-08-11

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A07250
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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