Anti-APO-E Antibody

Product Name

Anti-APO-E Antibody

View all APOE Antibodies

SKU/Catalog Number

A00015-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-APO-E Antibody (Catalog # A00015-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

APO-E antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-APO-E Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00015-1)

Host

Rabbit

Contents

APO-E Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

APO-E antibody was raised against a 19 amino acid peptide near the carboxy terminus of human APO-E. The immunogen is located within amino acids 220 - 270 of APO-E.

Reactive Species

A00015-1 is reactive to APOE in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

36.2 kDa

Background of APOE

Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E (APO-E), a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. Defects in APO-E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00015-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

APO-E antibody can be used for detection of APO-E by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information APOE

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