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- Table of Contents
Apoe antibody (PB9986) performs well in IF, IHC, ICC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of Apolipoprotein E/APOE using anti-Apolipoprotein E/APOE antibody (PB9986).
Apolipoprotein E/APOE was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Apolipoprotein E/APOE Antibody (PB9986) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Apolipoprotein E/APOE was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Apolipoprotein E/APOE Antibody (PB9986) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Apolipoprotein E/APOE Antibody Picoband®
Apoe antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
Apoe antibody was positively detected by WB in:
mouse spleen tissue, mouse kidney tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Apoe antibody was positively detected by IHC in:
mouse liver tissue, rat liver tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Apoe antibody was positively detected by ICC/IF in:
HEPA1-6 cell
Apoe antibody was positively detected by IF in:
mouse liver tissue, rat liver tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Mouse
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Mouse, Rat
Immunocytochemistry/Immunofluorescence
5μg/ml
Mouse
Immunofluorescence
5μg/ml
Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Apolipoprotein E/APOE Antibody Picoband®
View all APOE Antibodies
View all APOE Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Apolipoprotein E recombinant protein (Position: D55-Q294). Mouse Apolipoprotein E shares 75% and 94.6% amino acid (aa) sequence identity with human and rat Apolipoprotein E, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36 kDa
Calculated Molecular Weight
35.9 kDa
Background of Apoe
APOE is also known as AD2 or LPG. The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Alternative splicing results in multiple transcript variants.
Cite This Product
Anti-Apolipoprotein E/APOE Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9986)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
95%
High Homology
Monkey
75%
Moderate Homology
Human
75%
Moderate Homology
Rabbit
72%
Moderate Homology
Sheep
70%
Moderate Homology
Cow
70%
Moderate Homology
Dog
67%
Low homology
Zebrafish
28%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Apoe antibody
Immunohistochemistry (IHC) protocol for Apoe antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for Apoe antibody
Immunofluorescence (IF) protocol for Apoe antibody
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Product Promise
We promise Anti-Apolipoprotein E/APOE Antibody Picoband® performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9986
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026