Product Info Summary
SKU: | EZ1455 |
---|---|
Size: | For 5 plates, 96 wells each |
Reactive Species: | Human |
Application: | ELISA |
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Product info
Product Name
Human APOE ELISA Kit EZ-Set™ (DIY Antibody Pairs)
SKU/Catalog Number
EZ1455
Size
For 5 plates, 96 wells each
*Question: How many samples can I assay/run in this kit?
Description
Human APOE ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human APOE in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, milk and urine.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human APOE ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ1455)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: K19-H317
Assay Range
3.12 ng/ml - 200 ng/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EZ1455 is reactive to APOE in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, milk and urine.
Application Guarantee
EZ1455 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Catalog number | Description | Quantity | Storage of opened/reconstituted material |
---|---|---|---|
EZ1455-CA | Rabbit anti- human APOE polyclonal antibody (Capture Antibody) | 500 µl, 0.4 mg/mL (recommended dilution 1:100) | Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. |
EZ1455-DA | Biotinylated goat anti- human APOE polyclonal antibody (Detection Antibody) | 500 µl, 2 µg/mL (recommended dilution 1:100) | |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 500 µl (recommended dilution 1:100) | |
EK1455-ST | Lyophilized recombinant human APOE standard | 200 ng/tube×3 | Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Incubator.
- 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 5. Clean tubes and Eppendorf tubes.
- 6. 96 well microplate (Cat# AR1100)
- 7. Plate Sealers.
- 8. Capture Antibody Diluent: PBS.
- 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- 11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
- 12. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. - *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration (ng/ml) | 0 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 | 200 |
O.D. | 0.01 | 0.073 | 0.138 | 0.243 | 0.484 | 0.907 | 1.475 | 2.004 |
Data Example Images
![ez1455 ez1455](https://www.bosterbio.com/media/catalog/product/e/z/ez1455.png)
Click image to see more details
Human APOE EZ-Set ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 7334 | 30860 | 101114 | 7480 | 33927 | 97046 |
Standard deviation | 506.04 | 2191.06 | 7886.89 | 598.4 | 2917.72 | 7957.77 |
CV (%) | 6.9% | 7.1% | 7.8% | 8% | 8.6% | 8.2% |
Protein Target Info & Infographic
Gene/Protein Information For APOE (Source: Uniprot.Org, NCBI)
Gene Name
APOE
Full Name
Apolipoprotein E
Weight
Superfamily
apolipoprotein A1/A4/E family
Alternative Names
APOE; Apo-E; Apolipoprotein E; apolipoprotein E3; late onset); MGC1571 APOE AD2, APO-E, ApoE4, LDLCQ5, LPG apolipoprotein E apolipoprotein E|apolipoprotein E3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on APOE, check out the APOE Infographic
![APOE infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for APOE: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human APOE ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ1455)
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