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Product Info Summary
| SKU: | A00015 |
|---|---|
| Size: | 500ug |
| Reactive Species: | Human, Rat |
| Host: | Goat |
| Application: | ELISA, IP, IHC, WB |
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Product info
Product Name
Anti-APOLIPOPROTEINE APOE Antibody
SKU/Catalog Number
A00015
Size
500ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-APOLIPOPROTEINE APOE Antibody (Catalog # A00015). Tested in WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening. (Ship on wet ice.)
Cite This Product
Anti-APOLIPOPROTEINE APOE Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00015)
Host
Goat
Contents
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
Apolipoprotein Type E was isolated from human plasma by density gradient centrifugation followed by HPLC purification.
Cross-reactivity
beta-Actin antibody is human, mouse, rat, rabbit, chicken, zebrafish and drosophila reactive.
Reactive Species
A00015 is reactive to APOE in Human, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
36.2 kDa
Background of APOE
Anti Apolipoprotein E Antibody recognizes the gene product of APOE. Apolipoprotein E is found in the chylomicron and IDLs that binds to a specific receptor on liver cells and peripheral cells. Apolipoprotein E is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This antibody is suitable for cardiovascular research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00015 is guaranteed for ELISA, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:4,000 - 1:8,000
IHC: 1:50 - 1:200
IP: 1:100
WB: 1:500 - 1:2,000
Apolipoprotein E antibodies have been tested by western blot and used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.
Validation Images & Assay Conditions
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Western Blot of goat Anti Apolipoprotein E Antibody. Lane 1: rat glial whole cell lysate. Load: 40 µg per lane. Primary antibody: Apolipoprotein E antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP goat secondary antibody at 1:200 for 45 min at RT. Block: 3%BLOTTO overnight at 4°C. Predicted/Observed size: 36 kDa, 35-36 kDa for APO-E. MW markers arrows at 25kDa and 37kDa. Other band(s): ~55kDa.
Specific Publications For Anti-APOLIPOPROTEINE APOE Antibody (A00015)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-APOLIPOPROTEINE APOE Antibody
Question
Is there a BSA free version of anti-APOLIPOPROTEINE antibody A00015 available?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-APOLIPOPROTEINE antibody A00015 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-25
Question
My colleagues were well pleased with the WB result of your anti-APOLIPOPROTEINE antibody. However we have seen positive staining in cerebrospinal fluid secreted. using this antibody. Is that expected? Could you tell me where is APOE supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
From literature, cerebrospinal fluid does express APOE. Generally APOE expresses in secreted. Regarding which tissues have APOE expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 6897404, 7068630, 6860692
Cerebellum, Pubmed ID: 14702039
Cerebrospinal fluid, Pubmed ID: 19838169
Eye, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-12-24
Question
Is this A00015 anti-APOLIPOPROTEINE antibody reactive to the isotypes of APOE?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
The immunogen of A00015 anti-APOLIPOPROTEINE antibody is Apolipoprotein Type E was isolated from human plasma by density gradient centrifugation followed by HPLC purification. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-01
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-APOLIPOPROTEINE antibody A00015. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-23
Question
I have a question about product A00015, anti-APOLIPOPROTEINE antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00015 anti-APOLIPOPROTEINE antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-14
Question
We want to test anti-APOLIPOPROTEINE antibody A00015 on human liver for research purposes, then I may be interested in using anti-APOLIPOPROTEINE antibody A00015 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
The products we sell, including anti-APOLIPOPROTEINE antibody A00015, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-08
Question
Is a blocking peptide available for product anti-APOLIPOPROTEINE antibody (A00015)?
Verified Customer
Verified customer
Asked: 2018-10-04
Answer
We do provide the blocking peptide for product anti-APOLIPOPROTEINE antibody (A00015). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-10-04
Question
We have seen staining in rat cerebrospinal fluid. What should we do? Is anti-APOLIPOPROTEINE antibody supposed to stain cerebrospinal fluid positively?
Verified Customer
Verified customer
Asked: 2018-05-24
Answer
From literature cerebrospinal fluid does express APOE. From Uniprot.org, APOE is expressed in left adrenal gland, cerebellum, eye, blood, cerebrospinal fluid, liver, among other tissues. Regarding which tissues have APOE expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 6897404, 7068630, 6860692
Cerebellum, Pubmed ID: 14702039
Cerebrospinal fluid, Pubmed ID: 19838169
Eye, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2018-05-24
Question
I have attached the WB image, lot number and protocol we used for liver using anti-APOLIPOPROTEINE antibody A00015. Please let me know if you require anything else.
J. Gonzalez
Verified customer
Asked: 2017-12-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-22
Question
Would anti-APOLIPOPROTEINE antibody A00015 work for WB with liver?
Verified Customer
Verified customer
Asked: 2017-12-20
Answer
According to the expression profile of liver, APOE is highly expressed in liver. So, it is likely that anti-APOLIPOPROTEINE antibody A00015 will work for WB with liver.
Boster Scientific Support
Answered: 2017-12-20
Question
I see that the anti-APOLIPOPROTEINE antibody A00015 works with WB, what is the protocol used to produce the result images on the product page?
P. Miller
Verified customer
Asked: 2017-10-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-10-17
Question
I was wanting to use your anti-APOLIPOPROTEINE antibody for WB for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
D. Wu
Verified customer
Asked: 2015-09-18
Answer
It shows on the product datasheet, A00015 anti-APOLIPOPROTEINE antibody has been tested for IP, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-09-18
Question
We ordered your anti-APOLIPOPROTEINE antibody for IP on cerebellum in a previous experiment. I am using human, and We want to use the antibody for WB next. We want examining cerebellum as well as cerebrospinal fluid in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
K. Johnson
Verified customer
Asked: 2013-04-05
Answer
I have checked the website and datasheets of our anti-APOLIPOPROTEINE antibody and it seems that A00015 has been tested on human in both IP and WB. Thus A00015 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-04-05
Question
We are currently using anti-APOLIPOPROTEINE antibody A00015 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on primate tissues as well?
V. Anderson
Verified customer
Asked: 2013-01-31
Answer
The anti-APOLIPOPROTEINE antibody (A00015) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-01-31
Question
Does A00015 anti-APOLIPOPROTEINE antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Evans
Verified customer
Asked: 2013-01-29
Answer
It shows on the product datasheet, A00015 anti-APOLIPOPROTEINE antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-01-29
