Anti-Arginase-2, mitochondrial Arg2 Antibody Picoband®

Product Name

Anti-Arginase-2, mitochondrial Arg2 Antibody Picoband®

View all ARG2 Antibodies

SKU/Catalog Number

PA1805
BA3797 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Arginase-2, mitochondrial Arg2 Antibody catalog # PA1805. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Arginase-2, mitochondrial Arg2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1805)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Arg2, different from the related mouse sequence by three amino acids and from the related rat sequence by two amino acids.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1805 is reactive to ARG2 in Human

Observed Molecular Weight

38 kDa

Calculated molecular weight

38.6 kDa

Background of ARG2

ARG2 (arginase, type II) encodes a 355-amino acid polypeptide. Using Northern blotting and RT-PCR, Vockley et al. (1996) found that ARG2 is expressed as a 1.5-kb mRNA in a wide variety of tissues, with highest levels of expression in prostate, brain, and kidney. By PCR analysis of somatic cell hybrid panels, fluorescence in situ hybridization, and radiation hybrid analysis, the ARG2 gene is mapped to 14q24.1-q24.3. ARG2 may be inducible and may be essential in the regulation of nitric oxide synthesis by modulating local arginine concentrations. Gotoh et al. (1996) showed that ARG2 mRNA and nitric oxide synthase (NOS) mRNA were coinduced by lipopolysaccharide in a macrophage-like cell line. Arginase II has been implicated in the regulation of the arginine/ornithine concentrations in the cell. The mitochondrial location of ARG2 and its coinduction with ornithine aminotransferase and involvement with proline biosynthesis in lactating rat mammary gland had led to the inference that ARG2 is involved in biosynthetic functions, as opposed to the metabolic ones of the urea cycle.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1805 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human

Positive Control

WB: human 293T whole cell, human PC-3 whole cell, human K562 whole cell, human HepG2 whole cell
IHC: human prostate cancer tissue

Validation Images & Assay Conditions

See full target information ARG2

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