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Product Info Summary
| SKU: | M01106-4 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-liver Arginase/ARG1 Antibody Picoband® (monoclonal, 2B12)
SKU/Catalog Number
M01106-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-liver Arginase/ARG1 Antibody Picoband® (monoclonal, 2B12) catalog # M01106-4. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-liver Arginase/ARG1 Antibody Picoband® (monoclonal, 2B12) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01106-4)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
2B12
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human liver Arginase/ARG1 recombinant protein (Position: E25-D183).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M01106-4 is reactive to ARG1 in Human, Monkey, Mouse, Rat
Observed Molecular Weight
35 kDa
Calculated molecular weight
34.7 kDa
Background of ARG1
ARG1 (arginase, live) is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. The isoform encoded byARG1, referred to as the liver, or A-I, isoform, contributes 98% of the arginase activity in liver but is also present in red cells. Using a rat liverARG1cDNA clone to probe a human liver cDNA library,Haraguchi et al. (1987)isolated and characterized a cDNA corresponding to theARG1gene.The ARG1 gene is mapped on 6q23.2 and the arginase gene contains 8 exons. By immunologic studies, 90% of the arginase in red blood cell and liver was precipitated by the antibody, whereas only 50% of the arginase in kidney, brain, and the gastrointestinal tract reacted with it. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01106-4 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Monkey, Mouse, Rat
Flow Cytometry (Fixed), 1-3μ/1x106 cells, Human
Positive Control
WB: rat liver tissue, mouse liver tissue, monkey liver tissue
FCM: Jurkat cell, SiHa cell
Validation Images & Assay Conditions
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Western blot analysis of liver Arginase/ARG1 using anti-liver Arginase/ARG1 antibody (M01106-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates,
Lane 3: monkey liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-liver Arginase/ARG1 antigen affinity purified monoclonal antibody (Catalog # M01106-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for liver Arginase/ARG1 at approximately 35KD. The expected band size for liver Arginase/ARG1 is at 35KD.
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Flow Cytometry analysis of Jurkat cells using anti- liver Arginase/ARG1 antibody (M01106-4).
Overlay histogram showing Jurkat cells stained with M01106-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-liver Arginase/ARG1 Antibody (M01106-4, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Flow Cytometry analysis of SiHa cells using anti- liver Arginase/ARG1 antibody (M01106-4).
Overlay histogram showing SiHa cells stained with M01106-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-liver Arginase/ARG1 Antibody (M01106-4, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-liver Arginase/ARG1 Antibody Picoband® (monoclonal, 2B12) (M01106-4)
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