Product Info Summary
SKU: | M01106-4 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Monkey, Mouse, Rat |
Host: | Mouse |
Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-liver Arginase/ARG1 Antibody Picoband™ (monoclonal, 2B12)
View all Arginase 1/ARG1/liver Arginase Antibodies
SKU/Catalog Number
M01106-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-liver Arginase/ARG1 Antibody Picoband™ (monoclonal, 2B12) catalog # M01106-4. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-liver Arginase/ARG1 Antibody Picoband™ (monoclonal, 2B12) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01106-4)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
2B12
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human liver Arginase/ARG1 recombinant protein (Position: E25-D183).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M01106-4 is reactive to ARG1 in Human, Monkey, Mouse, Rat
Applications
M01106-4 is guaranteed for Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
35 kDa
Calculated molecular weight
34.735kDa
Background of Arginase 1/ARG1/liver Arginase
ARG1 (arginase, live) is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. The isoform encoded byARG1, referred to as the liver, or A-I, isoform, contributes 98% of the arginase activity in liver but is also present in red cells. Using a rat liverARG1cDNA clone to probe a human liver cDNA library,Haraguchi et al. (1987)isolated and characterized a cDNA corresponding to theARG1gene.The ARG1 gene is mapped on 6q23.2 and the arginase gene contains 8 exons. By immunologic studies, 90% of the arginase in red blood cell and liver was precipitated by the antibody, whereas only 50% of the arginase in kidney, brain, and the gastrointestinal tract reacted with it. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Monkey, Mouse, Rat
Flow Cytometry, 1-3μ/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of liver Arginase/ARG1 using anti-liver Arginase/ARG1 antibody (M01106-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates,
Lane 3: monkey liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-liver Arginase/ARG1 antigen affinity purified monoclonal antibody (Catalog # M01106-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for liver Arginase/ARG1 at approximately 35KD. The expected band size for liver Arginase/ARG1 is at 35KD.
Click image to see more details
Figure 2. Flow Cytometry analysis of Jurkat cells using anti-liver Arginase/ARG1 antibody (M01106-4).
Overlay histogram showing Jurkat cells stained with M01106-4 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-liver Arginase/ARG1 Antibody (M01106-4, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 3. Flow Cytometry analysis of SiHa cells using anti-liver Arginase/ARG1 antibody (M01106-4).
Overlay histogram showing SiHa cells stained with M01106-4 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-liver Arginase/ARG1 Antibody (M01106-4, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For ARG1 (Source: Uniprot.org, NCBI)
Gene Name
ARG1
Full Name
Arginase-1
Weight
34.735kDa
Superfamily
arginase family
Alternative Names
AI; ARG1; Arginase 1; arginase, liver; Arginase-1; EC 3.5.3.1; Liver Arginase; Liver-type arginase; PGIF; Type I Arginase ARG1 arginase 1 arginase-1|arginase, liver|liver-type arginase|type I arginase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ARG1, check out the ARG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ARG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-liver Arginase/ARG1 Antibody Picoband™ (monoclonal, 2B12) (M01106-4)
Hello CJ!
M01106-4 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li T, Zhao J, Xie W, Yuan W, Guo J, Pang S, Gan WB, Gómez-Nicola D, Zhang S. Specific depletion of resident microglia in the early stage of stroke reduces cerebral ischemic damage. J Neuroinflammation. 2021 Mar 23;18(1):81. doi: 10.1186/s12974-021-02127-w. PMID: 33757565; PMCID: PMC7986495.
Species: Mouse
M01106-4 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:300
Liao H,Li Y,Zhang X,Zhao X,Zheng D,Shen D,Li R. Protective Effects of Thalidomide on High-Glucose-Induced Podocyte Injury through In Vitro Modulation of Macrophage M1/M2 Differentiation.J Immunol Res.2020 Aug 27;2020:8263598.doi:10.1155/2020/ 8263598.PMID
Species: Mouse
M01106-4 usage in article: APP:WB, SAMPLE:MACROPHAGES, DILUTION:1:500
Li Y,Zheng D,Shen D,Zhang X,Zhao X,Liao H.Protective Effects of Two Safflower Derived Compounds, Kaempferol and Hydroxysafflor Yellow A, on Hyperglycaemic Stress-Induced Podocyte Apoptosis via Modulating of Macrophage M1/M2 Polarization.J Immunol Res.2020
Species: Mouse
M01106-4 usage in article: APP:WB, SAMPLE:MACROPHAGES, DILUTION:1:500
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