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- Table of Contents
AHR antibody (A00225-4) performs well in ELISA, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 14 Publications
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IHC analysis of AHR using anti-AHR antibody (A00225-4).
AHR was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AHR Antibody (A00225-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
AHR was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AHR Antibody (A00225-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Designing a Western blot for AHR? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the AHR Western Blot GuideBundle Save
Anti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®
AHR antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC, ICC, WB |
Tested Applications
AHR antibody was positively detected by WB in:
human MCF-7 whole cell, human PC-3 whole cell, human A549 whole cell, human Hela whole cell, human Hela- WT whole cell, human Hela-AHR KO whole cell, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
AHR antibody was positively detected by IHC in:
human spleen tissue, human colon cancer tissue, mouse brain tissue, rat brain tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
AHR antibody was positively detected by ICC/IF in:
U2OS cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25μg/ml
Human, Mouse
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®
View all AHR Antibodies
View all AHR Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Aryl hydrocarbon Receptor/AHR recombinant protein (Position: A6-L842).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
100 kDa
Calculated Molecular Weight
96.1 kDa
Background of AHR
The aryl hydrocarbon receptor (AhR or AHR or ahr or ahR) is a protein that in humans is encoded by the AHR gene. It is mapped to 7p21.1. The protein encoded by this gene is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, the encoded protein is sequestered in the cytoplasm; upon ligand binding, this protein moves to the nucleus and stimulates transcription of target genes.
Cite This Product
Anti-Aryl hydrocarbon Receptor/AHR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00225-4)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
95%
High Homology
Dog
85%
Moderate Homology
Sheep
81%
Moderate Homology
Cow
80%
Moderate Homology
Rabbit
79%
Moderate Homology
Rat
71%
Moderate Homology
Mouse
70%
Moderate Homology
Zebrafish
48%
Low homology
Drosophila
31%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for AHR antibody
Immunohistochemistry (IHC) protocol for AHR antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for AHR antibody
Publications
Application:
Species:
IDO1 Inhibitor RY103 Prevents Sepsis-Associated Immunosuppression in Cecal Ligation and Puncture Mice
Published: 2026-08-13
Journal: Phenomics
Application: WB
Impact Factor: 5.50
Species: Mouse
Oral bovine osteopontin alters intestinal immune marker profiles in juvenile rats: concurrent changes in the aryl hydrocarbon receptor/signal transducer and activator of transcription 3/interleukin-6 axis
Published: 2026-07-10
Journal: Food Bioscience
Application: IF
Impact Factor: 6.20
Species: Rat
AHR signaling modulates intestinal and hepatic metabolic homeostasis in colitis: alterations in microbiome and bile acid profiles
Published: 2026-03-14
Journal: Food Bioscience
Application: IF
Impact Factor: 5.90
Species: Mouse
Excessive progesterone impairs mouse decidualization via the Kyn-AhR pathway
Published: 2025-09-22
Journal: Frontiers in Cell and Developmental Biology
Application: WB,IF
Impact Factor: 4.30
Species: Mouse
PMID: 41059340
Niuhuang Jiedu Tablet's compatibility alleviates realgar-induced intestinal toxicity by activating aryl hydrocarbon receptor pathway via Akkermansia and indole−3−lactate
Published: 2025-07-07
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 5.40
Species: Mouse
PMID: 40633858
Aryl Hydrocarbon Receptor Alleviates Hepatic Fibrosis by Inducing Hepatic Stellate Cell Ferroptosis
Published: 2024-12-09
Journal: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
Application: WB,ChIP
Impact Factor: 4.30
Species: Mouse
PMID: 39654034
AHR regulates liver enlargement and regeneration through the YAP signaling pathway
Published: 2024-09-02
Journal: Heliyon
Application: IF
Impact Factor: 3.40
Species: Mouse
PMID: 39296106
Indole-3-aldehyde Alleviates High-Fat Diet-Induced Gut Barrier Disruption by Increasing Intestinal Stem Cell Expansion
Published: 2024-08-15
Journal: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Application: WB
Impact Factor: 5.70
Species: Mouse
PMID: 39146439
Short-chain fatty acids suppresses astrocyte activation by amplifying Trp-AhR-AQP4 signaling in experimental autoimmune encephalomyelitis mice
Published: 2024-07-08
Journal: CELLULAR AND MOLECULAR LIFE SCIENCES
Application: IF,WB
Impact Factor: 6.20
Species: Mouse
PMID: 38976012
Aryl hydrocarbon receptor (AhR) is regulated by hyperoxia in premature infants
Published: 2024-05-30
Journal: Journal of Maternal-Fetal & Neonatal Medicine
Application: IF,WB
Impact Factor: 1.70
Species: Human
PMID: 38816997
Omeprazole activates aryl hydrocarbon receptor to reduce hyperoxia-induced oxidative stress in the peripheral blood mononuclear cells from premature infants
Published: 2023-10-26
Journal: Journal of Maternal-Fetal & Neonatal Medicine
Application: IF,WB
Impact Factor: 1.80
Species: Human
PMID: 37884440
Effects of Dietary Indole-3-carboxaldehyde Supplementation on Growth Performance, Intestinal Epithelial Function, and Intestinal Microbial Composition in Weaned Piglets
Published: 2022-05-16
Journal: Frontiers in Nutrition
Application: WB
Impact Factor: 6.59
Species: Pig
PMID: 35651506
Dynamic changes of activated AHR in microglia and astrocytes in the substantia nigra-striatum system in an MPTP-induced Parkinson’s disease mouse model
Published: 2021-09-01
Journal: BRAIN RESEARCH BULLETIN
Application: WB,IF
Impact Factor: 4.08
Species: Mouse
PMID: 34478811
Aryl Hydrocarbon Receptor Suppresses the Prostate Cancer LNCaP Cell Growth and Invasion by Promoting DNA Damage Response Under Oxidative Stress
Published: 2017-11-01
Journal: DNA AND CELL BIOLOGY
Application: WB
Impact Factor: 2.24
Species: Human
PMID: 28972393
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Product Promise
We promise Anti-Aryl hydrocarbon Receptor/AHR Antibody Picoband® performs excellently in relevant applications for ELISA, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00225-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 17, 2026