Mouse IL-20/Interleukin-20 ELISA Kit PicoKine™

Mouse IL-20 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il20 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 3 publication(s).

Product Info Summary

SKU: EK1350
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse IL-20/Interleukin-20 ELISA Kit PicoKine™

See all IL-20 products

SKU/Catalog Number

EK1350

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse IL-20 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il20 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse IL-20/Interleukin-20 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1350)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: L25-E176

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1350 is reactive to Il20 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1350 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1350-CAP Anti-Mouse Il20 Pre-coated 96-well strip microplate 1
EK1350-ST Mouse Il20 Standard 2 vials, 10 ng/tube
EK1350-DA Mouse Il20 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)02000 2000 2000 2000 2000 2000 2000
O.D.0.1200.1860.2310.3370.5630.8921.6032.248

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse IL-20/Interleukin-20 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)9323299788234940
Standard deviation6.4111.666.796.8614.568.62
CV (%)6.9%5%6.7%7.8%6.2%7.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-20 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 193899083883.634.1%
Sample 22322462372252357.643.2%
Sample 399787489586090653.715.9%
*number of samples for each test n=16.

Gene/Protein Information For Il20 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9JKV9

Gene ID

58181

Gene Name

Il20

Full Name

Interleukin-20

Weight

20.098kDa

Superfamily

IL-10 family

Alternative Names

Cytokine Zcyto10; IL10D; IL20; IL-20; IL-20ZCYTO10four alpha helix cytokine; interleukin 20; interleukin-20; MGC96907 Il20|If2d, Zcyto1, Zcyto10|interleukin 20|interleukin-20|IL-20|cytokine Zcyto10|four alpha helix cytokine ZCYTO10|four helix bundle cytokine 10|interferon 2d

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il20, check out the Il20 Infographic

Il20 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il20: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK1350 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Li Y, Li Sy, Li Js, Deng L, Tian Yg, Jiang Sl, Wang Y, Wang Yy. Biol Pharm Bull. 2012;35(10):1752-60. Epub 2012 Aug 3. A Rat Model For Stable Chronic Obstructive Pulmonary Disease Induced By Cigarette Smoke Inhalation And Repetitive Bacterial Infe...

Zhou Qb, Jin Yl, Jia Q, Zhang Y, Li Ly, Liu P, Liu Yt. Inflammation. 2014 Feb;37(1):107-15. Doi: 10.1007/S10753-013-9717-9. Baicalin Attenuates Brain Edema In A Rat Model Of Intracerebral Hemorrhage.

Liang Y, Liu Hh, Chen Yj, Sun H. J Med Food. 2014 Apr;17(4):439-46. Doi: 10.1089/Jmf.2013.2846. Epub 2014 Mar 4. Antitumor Activity Of The Protein And Small Molecule Component Fractions From Agrocybe Aegerita Through Enhancement Of Cytokine Produc...

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5 Customer Q&As for Mouse IL-20/Interleukin-20 ELISA Kit PicoKine™

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Z. Zhang

Verified customer

Asked: 2020-06-29

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-06-29

Question

Q: What is the optimal O.D. value for IL-20 ELISA kit? I performed your IL-20 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain IL-20 even though the O.D. values are not very high?

G. Kapoor

Verified customer

Asked: 2019-01-25

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-20 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of IL-20 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2019-01-25

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

A. Tiwari

Verified customer

Asked: 2018-07-18

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-07-18

Question

Q: can you suggest the dilution ratio of serum samples for detection of IL-20 in Mouse serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2018-06-24

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-20 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-06-24

Question

Q: is it okay to use citrate plasma as samples in Mouse IL-20 Picokine® ELISA Kit (Catalog # EK1350)?

Verified Customer

Verified customer

Asked: 2018-01-09

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of IL-20 causing degradation of its protein structure. IL-20 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the IL-20 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-01-09

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