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Product Info Summary
| SKU: | PB9419 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®
SKU/Catalog Number
PB9419
PB0387 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Aryl hydrocarbon Receptor/AHR Antibody Picoband® catalog # PB9419. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Aryl hydrocarbon Receptor/AHR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9419)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse AHR, different from the related human sequence by five amino acids, and from the related rat sequence by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9419 is reactive to Ahr in Mouse, Rat
Observed Molecular Weight
96 kDa
Calculated molecular weight
95.0 kDa
Background of Ahr
AHR (aryl hydrocarbon receptor), also called bHLHe76, is a member of the family of basic helix-loop-helix transcription factors. AhR is a cytosolic transcription factor that is normally inactive, bound to several co-chaperones. The AHR gene is mapped on 7p21.1. Estrogenic actions of AHR agonists were detected in wildtype ovariectomized mouse uteri, but were absent in Ahr -/- or Er-alpha -/- ovariectomized mice. Complex assembly and ubiquitin ligase activity of CUL4B (AHR) in vitro and in vivo are dependent on the AHR ligand. In the CUL4B (AHR) complex, ligand-activated AHR acts as a substrate-specific adaptor component that targets sex steroid receptors for degradation. Cd4-positive cells from mice lacking Ahr developed Th17 responses but failed to produce Il22 and did not show enhanced Th17 development. Activation of Ahr during induction of EAE accelerated disease onset and increased pathology in wildtype mice, but not in Ahr -/- mice. The TDO-AHR pathway is active in human brain tumors and is associated with malignant progression and poor survival. Ahr activity within ROR-gamma-t-positive ILC could be induced by dietary ligands such as those contained in vegetables of the family Brassicaceae.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9419 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, Mouse Liver Tissue
Validation Images & Assay Conditions
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Western blot analysis of AHR using anti-AHR antibody (PB9419).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Brain Tissue Lysate at 50ug,
Lane 2: Mouse Liver Tissue Lysate at 50ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AHR antigen affinity purified polyclonal antibody (Catalog # PB9419) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AHR at approximately 96 kDa. The expected band size for AHR is at 96 kDa.
Specific Publications For Anti-Aryl hydrocarbon Receptor/AHR Antibody Picoband® (PB9419)
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4 Customer Q&As for Anti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®
Question
We are currently using anti-Aryl hydrocarbon Receptor/AHR antibody PB9419 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
The anti-Aryl hydrocarbon Receptor/AHR antibody (PB9419) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-23
Question
We have seen staining in mouse liver. Any tips? Is anti-Aryl hydrocarbon Receptor/AHR antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
According to literature liver does express AHR. According to Uniprot.org, AHR is expressed in placenta, liver, among other tissues. Regarding which tissues have AHR expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 8393992
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-13
Question
Our team were content with the WB result of your anti-Aryl hydrocarbon Receptor/AHR antibody. However we have seen positive staining in placenta cytoplasm. using this antibody. Is that expected? Could you tell me where is AHR supposed to be expressed?
H. Baker
Verified customer
Asked: 2019-01-30
Answer
According to literature, placenta does express AHR. Generally AHR expresses in cytoplasm. Regarding which tissues have AHR expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 8393992
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-01-30
Question
My lab would like using your anti-Aryl hydrocarbon Receptor/AHR antibody for circumferential growth involved in left ventricle morphogenesis studies. Has this antibody been tested with western blotting on rat brain tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-05-19
Answer
Thanks for your inquiry. This PB9419 anti-Aryl hydrocarbon Receptor/AHR antibody is tested on rat brain tissue, tissue lysate, mouse liver tissue. It is guaranteed to work for WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-05-19
