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Product Info Summary
| SKU: | M32270 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-ATP5F1B Mouse Monoclonal Antibody [Clone ID: OTI1F7]
SKU/Catalog Number
M32270
Size
100 µl
Description
Boster Bio Anti-ATP5B mouse monoclonal antibody, clone OTI1F7 (formerly 1F7). Catalog# M32270. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Monkey, Dog.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATP5F1B Mouse Monoclonal Antibody [Clone ID: OTI1F7] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M32270)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1F7
Isotype
IgG1
Immunogen
Full length human recombinant protein of human ATP5B (NP_001677) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M32270 is reactive to ATP5F1B in Dog, Human, Monkey
Applications
M32270 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
56.6 kDa
Calculated molecular weight
56.56kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Validation Images & Assay Conditions
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Flow cytometric analysis of Jurkat cells
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HEK293T cells transfected with either pCMV6-ENTRY ATP5B (Red) or empty vector control plasmid (Blue) were immunostained with anti-ATP5B mouse monoclonal (M32270)
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Flow cytometric analysis of Hela cells
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Anti-ATP5B mouse monoclonal antibody (M32270) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP5B.
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Immunofluorescent staining of HepG2 cells using anti-ATP5B mouse monoclonal antibody (M32270).
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Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-ATP5Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-ATP5B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ATP5Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-ATP5Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-ATP5Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATP5B monoclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For ATP5F1B (Source: Uniprot.org, NCBI)
Gene Name
ATP5F1B
Full Name
ATP synthase subunit beta, mitochondrial
Weight
56.56kDa
Superfamily
ATPase alpha/beta chains family
Alternative Names
ATP synthase subunit beta, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide; ATPMB; ATPSBmitochondrial ATP synthase beta subunit; EC 3.6.3; EC 3.6.3.14; MGC5231; mitochondrial ATP synthetase, beta subunit ATP5F1B ATP5B, ATPMB, ATPSB, HEL-S-271 ATP synthase F1 subunit beta ATP synthase subunit beta, mitochondrial|ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide|epididymis secretory protein Li 271|mitochondrial ATP synthase beta subunit|mitochondrial ATP synthetase, beta subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATP5F1B, check out the ATP5F1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATP5F1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATP5F1B Mouse Monoclonal Antibody [Clone ID: OTI1F7] (M32270)
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