This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Gstp1 antibody (A00394-1) performs well in ELISA, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
Filter:
IHC analysis of GSTP1 using anti-GSTP1 antibody (A00394-1).
GSTP1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSTP1 Antibody (A00394-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
GSTP1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSTP1 Antibody (A00394-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Bundle Save
Anti-GST3/GST pi/Gstp1 Antibody Picoband®
Gstp1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC, ICC, WB |
Tested Applications
Gstp1 antibody was positively detected by WB in:
human Hela whole cell, human 293T whole cell, human Jurkat whole cell, rat liver tissue, rat stomach tissue, mouse liver tissue, mouse stomach tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Gstp1 antibody was positively detected by IHC in:
human liver cancer tissue, rat kidney tissue, rat liver tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Gstp1 antibody was positively detected by ICC/IF in:
NRK cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunocytochemistry/Immunofluorescence
5 μg/ml
ELISA
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Product Information
Product Name
Anti-GST3/GST pi/Gstp1 Antibody Picoband®
View all GSTP1 Antibodies
View all GSTP1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat GST3 / GST pi recombinant protein (Position: P2-Q210).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
23 kDa
Calculated Molecular Weight
23.4 kDa
Background of Gstp1
Glutathione S-transferases pi (GSTP1), also known as GST3, is an enzyme that in humans is encoded by the GSTP1 gene. This gene is mapped to 11q13.2. GSTP1 has 7 exons and 6 introns contained within approximately 2.8 kilobases. GSTP1 belongs to Glutathione S-transferases (GSTs) which are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 presents in all tissues and cells, with the exception of red cells, in which only erythrocyte GST (GSTe) is observed. What's more, GSTP1 is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.
Cite This Product
Anti-GST3/GST pi/Gstp1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00394-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Rat
100%
High Homology
Mouse
92%
High Homology
Dog
87%
Moderate Homology
Monkey
86%
Moderate Homology
Human
86%
Moderate Homology
Cow
86%
Moderate Homology
Rabbit
84%
Moderate Homology
Zebrafish
59%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Gstp1 antibody
Immunohistochemistry (IHC) protocol for Gstp1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for Gstp1 antibody
Publications
Application:
Glutathione S-Transferases and Cytochrome P450 enzyme expression in patients with Intracranial Tumors: Preliminary Report of 55 Patients
PMID: 30321868
Enhanced expression of stem cell markers and drug resistance in sphere-forming non-small cell lung cancer cells.
PMID: 26261505
The recovery trajectory of adolescent social defeat stress-induced behavioral, (1)H-MRS metabolites and myelin changes in Balb/c mice
PMID: 27283029
Yang P, Gao Z, Zhang H, Fang Z, Wu C, Xu H, Huang Qj. Neuropsychiatr Dis Treat. 2015 Mar 6;11:597-607. Doi: 10.2147/Ndt.S78131. Ecollection 2015. Changes In Proinflammatory Cytokines And White Matter In Chronically Stressed Rats.
PMID: 25834438
Review
Have you used Anti-GST3/GST pi/Gstp1 Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-GST3/GST pi/Gstp1 Antibody Picoband® performs excellently in relevant applications for ELISA, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A00394-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026