Product Info Summary
SKU: | M12432-1 |
---|---|
Size: | 100 µl |
Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
Host: | Mouse |
Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-ATP6V1F Mouse Monoclonal Antibody [Clone ID: OTI5F10]
View all V-type proton ATPase subunit F Antibodies
SKU/Catalog Number
M12432-1
Size
100 µl
Description
Boster Bio ATP6V1F mouse monoclonal antibody, clone OTI5F10 (formerly 5F10). Catalog# M12432-1. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-ATP6V1F Mouse Monoclonal Antibody [Clone ID: OTI5F10] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12432-1)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI5F10
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human ATP6V1F (NP_004222) produced in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M12432-1 is reactive to ATP6V1F in Dog, Human, Monkey, Mouse, Rat
Applications
M12432-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
13.37kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1F (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP6V1F.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATP6V1F monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ATP6V1F mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ATP6V1F mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either ATP6V1F (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ATP6V1F antibody (M12432-1)
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Anti-ATP6V1F mouse monoclonal antibody (M12432-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP6V1F.
Protein Target Info & Infographic
Gene/Protein Information For ATP6V1F (Source: Uniprot.org, NCBI)
Gene Name
ATP6V1F
Full Name
V-type proton ATPase subunit F
Weight
13.37kDa
Superfamily
V-ATPase F subunit family
Alternative Names
ATP6S14adenosinetriphosphatase 14k chain; ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F; H(+)-transporting two-sector ATPase, 14kD subunit; MGC117321; MGC126037; MGC126038; vacuolar ATP synthase subunit F; vacuolar proton pump F subunit; Vacuolar proton pump subunit F; VATFATPase, vacuolar, 14 kD; V-ATPase 14 kDa subunit; V-ATPase F subunit; V-ATPase subunit F; Vma7; V-type proton ATPase subunit F ATP6V1F ATP6S14, VATF, Vma7 ATPase H+ transporting V1 subunit F V-type proton ATPase subunit F|ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F|ATPase, vacuolar, 14 kD|H(+)-transporting two-sector ATPase, 14kD subunit|V-ATPase 14 kDa subunit|V-ATPase F subunit|V-ATPase subunit F|adenosinetriphosphatase 14k chain|vacuolar ATP synthase subunit F|vacuolar proton pump F subunit|vacuolar proton pump subunit F
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATP6V1F, check out the ATP6V1F Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATP6V1F: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATP6V1F Mouse Monoclonal Antibody [Clone ID: OTI5F10] (M12432-1)
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