This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
IL1B antibody (M00101-1) performs well in WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
Filter:
Western blot analysis of extracts from recombinant IL1 beta, using IL1 beta antibody.
Bundle Save
Anti-IL1 beta Rabbit Monoclonal Antibody
IL1B antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | WB |
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-IL1 beta Rabbit Monoclonal Antibody
View all IL1B Antibodies
View all IL1B Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BII-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IL1 beta
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
17 kDa
Calculated Molecular Weight
30.7 kDa
Cite This Product
Anti-IL1 beta Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00101-1)
Publications
Application:
Species:
A breakthrough in periodontitis treatment: Revealing the pharmacodynamic substances and mechanisms of Kouqiangjie formula
Published: 2024-01-09
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 4.80
Species: Rat
PMID: 38199336
Review
Have you used Anti-IL1 beta Rabbit Monoclonal Antibody?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
1 Review For Anti-IL1 beta Rabbit Monoclonal Antibody
IL-6 and IL-1β levels in the pancreas are key for assessing the anti-inflammatory effect of this nanomedicine. Antibodies from two previous suppliers showed poor specificity, while BosterBio antibodies proved highly specific, potent, and cost-effective.
| SKU | M00101-1 |
|---|---|
| Application | Western Blot |
| Sample | Mouse pancreatic acinar cells |
| Sample Processing Description | After centrifugation, collect the supernatant. Take a small portion for protein quantification using the BCA assay. Mix the remaining protein solution with an equal volume of loading buffer and denature in a 100°C water bath for 5 mins. |
| Primary Incubation | The membrane was incubated with the IL-6 and IL-1β primary antibodies (1:1000) overnight at 4 °C. |
| Secondary Antibody | HRP-conjugated Goat Anti-Rabbit IgG Secondary Antibody |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Other Reagents used | Protein-Free Blocking Buffer |
| Detection | Signal was developed using ECL substrate on a ChemiDoc MP system. |
| Results Summary | IL-6 and IL-1β levels in the pancreas are key for assessing the anti-inflammatory effect of this nanomedicine. Antibodies from two previous suppliers showed poor specificity, while BosterBio antibodies proved highly specific, potent, and cost-effective. In our subsequent experiments, our group continued to use BosterBio products, which proved to be highly reliable and provided solid support for the publication of several articles. |
Jiahui Yan, College of Chemistry and Chemical Engineering, Ocean University of China
Verified customer
Submitted 2025-09-25
Product Promise
We promise Anti-IL1 beta Rabbit Monoclonal Antibody performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00101-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026