Human IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™

Human IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL1B in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 0.15pg/ml. Cited in 58 publication(s). Independently reviewed in 2 review(s).

Product Info Summary

SKU: EK0392
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Human IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™

See all IL-1 beta/IL-1F2 products

SKU/Catalog Number

EK0392

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL1B in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 0.15pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0392)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A117-S269

Sensitivity

<0.15 pg/ml

Assay Range

3.9 pg/ml - 250 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0392 is reactive to IL1B in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK0392 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0392-CAP Anti-Human IL1B Pre-coated 96-well strip microplate 1
EK0392-ST Human IL1B Standard 2 vials, 1 ng/tube
EK0392-DA Human IL1B Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03.97.815.631.262.5125250
O.D.0.0220.0740.1050.2490.4230.7071.0951.830

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-1 beta/IL-1F2, Dilution ratio of 1:1.

Some articles we found to cite concentrations of IL-1 beta/IL-1F2 in samples: 22068351 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)11601231058112
Standard deviation0.452.888.970.573.249.96
CV (%)4.1%4.8%7.3%5.7%5.6%8.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-1 beta/IL-1F2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 111101111100.434.3%
Sample 260545253543.115.7%
Sample 31231191231121194.493.7%
*number of samples for each test n=16.

Gene/Protein Information For IL1B (Source: Uniprot.Org, NCBI)

Uniprot ID

P01584

Gene ID

3553

Gene Name

IL1B

Full Name

Interleukin-1 beta

Weight

30.748kDa

Superfamily

IL-1 family

Alternative Names

catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta IL1B IL-1, IL1-BETA, IL1F2, IL1beta interleukin 1 beta interleukin-1 beta|IL-1 beta|catabolin|interleukin 1beta|preinterleukin 1 beta|pro-interleukin-1-beta

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL1B, check out the IL1B Infographic

IL1B infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL1B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0392 has been cited in 58 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Lu F,Chen H,Hong Y,Lin Y,Liu L,Wei N,Wu Q,Liao S,Yang S,He J,Shao Y.A gain-of-function NLRP3 3‘-UTR polymorphism causes miR-146a-mediated suppression of NLRP3 expression and confers protection against sepsis progression.Sci Rep.2021 Jun 25;11(1):13300.doi:10.1038/s41598-021-92547-8.PMID: 34172780.
Species: Human
EK0392 usage in article: APP:ELISA, SAMPLE:PLASMA,HUVECS AND THP-1 CELL, DILUTION:NA

Amal Abdallah A.Abo-Elmagd,Dina Sabry,Ebtehal Mohammed,Interleukin-1β activity in gingival crevicular fluid of abutment teeth with temporary fixed restorations versus final fixed restorations: Prospective observational study,The Saudi Dental Journal,2021,,ISSN 1013-9052,https://doi.org/10.1016/j.sdentj.2021.06.001.
Species: Human
EK0392 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Activation of human hepatic stellate cells enhances the metastatic ability of hepatocellular carcinoma cells via upregulation of interleukin-1β. Bianqiao Cheng,Zhiteng Huang,Linlin Wu,Yanchen Lin,Weiguo Lin,Qi Zhu
Species: Human
EK0392 usage in article: APP:ELISA, SAMPLE:SMMC7721 CELL, DILUTION:NA

Pınar Bürhan-Çavuşoğlu,Evin İşcan,Ayşim Güneş,Neşe Atabey,Tunç Alkın,Increased telomerase activity in major depressive disorder with melancholic features: Possible role of pro-inflammatory cytokines and the brain-derived neurotrophic factor,Brain,Behavior,& Immunity - Health,2021,100259,ISSN 2666-3546,https://doi.org/10.1016/j.bbih.2021.100259.
Species: Human
EK0392 usage in article: APP:ELISA, SAMPLE:PLASMA, DILUTION:NA

Ma Z,Li K,Chen P,Pan J,Li X,Zhao G.Propofol Attenuates Inflammatory Damage via Inhibiting NLRP1-Casp1-Casp6 Signaling in Ischemic Brain Injury.Biol Pharm Bull.2020;43(10):1481-1489.doi:10.1248/bpb.b20-00050.PMID:32999158.
Species: Mouse
EK0392 usage in article: APP:ELISA, SAMPLE:CORTICAL NEURONS, DILUTION:NA

Yang M, Wang LI. MALAT1 knockdown protects from bronchial/tracheal smooth muscle cell injury via regulation of microRNA-133a/ryanodine receptor 2 axis. J Biosci. 2021;46:28. PMID: 33753579.
Species: Human,Rat
EK0392 usage in article: APP:ELISA, SAMPLE:B/TSMCS, DILUTION:NA

Du W, Wang L, Liao Z, Wang J. Circ_0085289 Alleviates the Progression of Periodontitis by Regulating let-7f-5p/SOCS6 Pathway. Inflammation. 2021 Mar 12. doi: 10.1007/s10753-021-01445-8. Epub ahead of print. PMID: 33710445.
Species: Human
EK0392 usage in article: APP:ELISA, SAMPLE:PDLCS, DILUTION:NA

Yudan Wang, Xiaomeng Ding, Yi Chen, Jianhua Xie, Bing Zheng, Xinxin Chang, Shiqi Liu, Xiaobo Hu, Qiang Yu,“Dialogue” between Caco-2 and DCs regulated by Ganoderma atrum polysaccharide in intestinal-like Caco-2/DCs co-culture model,Food Research International,2021,110310,ISSN 0963-9969,https://doi.org/10.1016/j.foodres.2021.110310.
Species: Human,Mouse
EK0392 usage in article: APP:ELISA, SAMPLE:CACO2 CELL, DILUTION:NA

Jia M,Lv Y,Xu Y,Gong Z.A comparative analysis of NLRP3-related inflammatory mediators in synovial fluid in temporomandibular joint osteoarthritis and internal derangement.BMC Musculoskelet Disord.2021 Feb 26;22(1):229.doi:10.1186/s12891-021-04092-0.PMID:3
Species: Human
EK0392 usage in article: APP:ELISA, SAMPLE:SYNOVIAL FLUID, DILUTION:NA

Guo L,Jiang B,Li D,Xiao X. Nephroprotective Effect of Adropinin Against Streptozotocin-Induced Diabetic Nephropathy in Rats: Inflammatory Mechanism and YAP/TAZ Factor.Drug Des Devel Ther.2021 Feb 16;15:589-600.doi:10. 2147/DDDT.S294009.PMID:33623368;PMCID
Species: Rat
EK0392 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Qinchao Chen,Meng Cao,Hanyi Ge,"Knockdown of MALAT1 Inhibits the Progression of Chronic Periodontitis via Targeting miR-769-5p/HIF3A Axis",BioMed Research International,vol.2021,Article ID 8899863,12 pages,2021.https://doi.org/10.1155/2021/8899863
Species: Human
EK0392 usage in article: APP:ELISA, SAMPLE:PDLCS, DILUTION:NA

Gao H,Guo Z. LncRNA XIST regulates atherosclerosis progression in ox-LDL-induced HUVECs. Open Med (Wars). 2021 Jan 8;16(1):117-127.doi:10.1515/med-2021-0200.PMID:33542956;PMCID: PMC7819546.
Species: Human
EK0392 usage in article: APP:ELISA, SAMPLE:HUVECS, DILUTION:NA

Wei W,Yao Y,Bi H,Xu W,Gao Y.Circular RNA circ_0068,888 protects against lipopolysaccharide-induced HK-2 cell injury via sponging microRNA-21-5p.Biochem Biophys Res Commun.2021 Jan 8;540:1-7.doi:10.1016/j.bbrc.2020.12.018.Epub ahead of print.PMID:33429194.
Species: Human
EK0392 usage in article: APP:ELISA, SAMPLE:HK-2 CELL, DILUTION:NA

Qiu M,Liu D,Fu Q.MiR-129-5p shuttled by human synovial mesenchymal stem cell-derived exosomes relieves IL-1β induced osteoarthritis via targeting HMGB1.Life Sci.2021 Jan 5:118987.doi:10.1016/j.lfs.2020.118987.Epub ahead of print.PMID:33417958.
Species: Human
EK0392 usage in article: APP:ELISA, SAMPLE:CHONDROCYTES, DILUTION:NA

Mengying Jia,Yaoguang Lv,Yingjie Xu et al.Pyroptosis Related Molecules are Significant for Cartilage Degeneration, 23 November 2020, PREPRINT (Version 1) available at Research Square [+https://doi.org/10.21203/rs.3.rs-104795/v1+]
Species: Human
EK0392 usage in article: APP:ELISA, SAMPLE:SYNOVIAL FLUID, DILUTION:NA

J. Med.Chem. 2021, 64, 1, 768–781 Publication Date:December 1, 2020
Species: Human,Mouse
EK0392 usage in article: APP:ELISA, SAMPLE:HEK-293 CELL, DILUTION:NA

Fu,C.,Xu,Q.,Tang,S.,Cao,Y.,Liu,C.,Wang,Y.,Qian,Y.,Shi,F.,Gui,J.,Fan,Q., & Ling,Y.(2020).The mobilization of splenic reservoir myeloid-derived suppressor cells in sepsis-induced myocardial injury.American journal of translational research, 12(11), 7114–712
Species: Human,Mouse
EK0392 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Tu CC, Wang SH, Hu CY, Chen MH, Hsieh O, Chang PC. Comparison of regular- and slope-configured dental implants placed in the edentulous ridge with height discrepancy: A pilot randomized controlled trial. J Formos Med Assoc. 2020 Nov 5:S0929-6646(20)30503-
Species: Human
EK0392 usage in article: APP:ELISA, SAMPLE:GINGIVAL CERVICULAR FLUID, DILUTION:NA

Dai Z,Chen XY,An LY,Li CC,Zhao N,Yang F,You ST,Hou CZ,Li K,Jiang C,You QD,Di B,Xu LL. Development of Novel Tetrahydroquinoline Inhibitors of NLRP3 Inflammasome for Potential Treatment of DSS-Induced Mouse Colitis. J Med Chem. 2020 Dec 17.doi: 10.1021/acs.
Species: Mouse
EK0392 usage in article: APP:ELISA, SAMPLE:THP-M CELL AND SERUM, DILUTION:NA

Xue M, Peng N, Zhu X, Zhang H. Hsa_circ_0006872 promotes cigarette smoke-induced apoptosis, inflammation and oxidative stress in HPMECs and BEAS-2B cells through the miR-145-5p/NF-κB axis. Biochem Biophys Res Commun. 2020 Nov 25:S0006-291X(20)32086-6. doi
Species: Human
EK0392 usage in article: APP:ELISA, SAMPLE:HPMECS AND BEAS-2B CELLS, DILUTION:NA

Yang, Q., Liu, Q., Lv, H., Wang, F., Liu, R., & Zeng, N. (2020). Effect of pulegone on the NLPR3 inflammasome during inflammatory activation of THP‑1 cells. Experimental and Therapeutic Medicine, 19(2), 1304-1312.

Ren, Z., Yang, K., Zhao, M., Liu, W., Zhang, X., Chi, J., ... & Yin, X. (2020). Calcium-sensing receptor on neutrophil promotes myocardial apoptosis and fibrosis after acute myocardial infarction via NLRP3 inflammasome activation. Canadian Journal of Card

Duan, Y., Zheng, H., Li, Z., Yao, Y., Ding, J., Wang, X., ... & Zheng, X. (2020). Unsaturated polyurethane films grafted with enantiomeric polylysine promotes macrophage polarization to a M2 phenotype through PI3K/Akt1/mTOR axis. Biomaterials, 120012.

Exogenous H2S protects H9c2 cardiac cells against high glucose-induced injury and inflammation by inhibiting the activation of the NF-%u03BAB and IL-1%u03B2 %u2026

Protection of SAL B with H9C2 cells

Increased Age-Related Cardiac Dysfunction in Bradykinin B2 Receptor%u2013Deficient Mice

HoxA13 Stimulates Myometrial Cells to Secrete IL-1%u03B2 and Enhance the Expression of Contraction-Associated Proteins

Expression and clinical significance of the NEK7-NLRP3 inflammasome signaling pathway in patients with systemic lupus erythematosus

Psoriasis 1'reduces psoriasis%u2011like skin inflammation by inhibiting the VDR%u2011mediated nuclear NF%u2011%u03BAB and STAT signaling pathways

Hypericin-photodynamic therapy induces human umbilical vein endothelial cell apoptosis

Pannexin3 inhibits TNF???induced inflammatory response by suppressing NF??B signalling pathway in human dental pulp cells

miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBP?

LL202 protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting MAPK/AP-1 signaling

NF-?B-Regulated miR-99a Modulates Endothelial Cell Inflammation.

Characteristics of CD4+CD25+Foxp3+?regulatory T cells in patients with multiple organ dysfunction syndrome

Associations of Trauma Severity with Mean Platelet Volume and Levels of Systemic Inflammatory Markers (IL1?, IL6, TNF?, and CRP)

GEN-27, a Newly Synthetic Isoflavonoid, Inhibits the Proliferation of Colon Cancer Cells in Inflammation Microenvironment by Suppressing NF-?B Pathway

High glucose induces and activates Toll-like receptor 4 in endothelial cells of diabetic retinopathy

TNF?-Mediated Loss of ?-Catenin/E-Cadherin Association and Subsequent Increase in Cell Migration Is Partially Restored by NKX3.1 Expression in Prostate Cells

Transcriptional Profiling Reveals Crosstalk Between Mesenchymal Stem Cells and Endothelial Cells Promoting Prevascularization by Reciprocal Mechanisms

Effects of Pantoprazole on Systemic and Gastric Pro- and Anti-inflammatory Cytokines in Critically Ill Patients

Hydrogen Sulfide Protects against Chemical Hypoxia-Induced Cytotoxicity and Inflammation in HaCaT Cells through Inhibition of ROS/NF-?B/COX-2 Pathway

Sun HJ, Chen D, Wang PY, Wan MY, Zhang CX, Zhang ZX, Lin W, Zhang F. Oxid Med Cell Longev. 2017;2017:6905217. doi: 10.1155/2017/6905217. Epub 2017 Nov 19. Salusin-β Is Involved in Diabetes Mellitus-Induced Endothelial Dysfunction via Degradation o...

Wan L, Yuan X, Liu M, Xue B. Exp Ther Med. 2018 Mar;15(3):2429-2435. doi: 10.3892/etm.2017.5667. Epub 2017 Dec 21. miRNA-223-3p regulates NLRP3 to promote apoptosis and inhibit proliferation of hep3B cells

Liu X, Zhang X, Ding Y, Zhou W, Tao L, Lu P, Wang Y, Hu R. Antioxid Redox Signal. 2017 Jan 1;26(1):28-43. doi: 10.1089/ars.2015.6615. Epub 2016 Aug 9. Nuclear Factor E2-Related Factor-2 Negatively Regulates NLRP3 Inflammasome Activity by Inhibitin...

Xuanfei L, Hao C, Zhujun Y, Yanming L, Jianping G. Oncotarget. 2017 Mar 28;8(13):21015-21030. doi: 10.18632/oncotarget.15472. Imidazoline I2 receptor inhibitor idazoxan regulates the progression of hepatic fibrosis via Akt-Nrf2-Smad2/3 signaling p...

Qin Q, Xu G, Qi W, Guo M, Wang Z, Xu W, Qiao Z, Gu Y, Niu J. Exp Ther Med. 2017 Aug;14(2):1017-1022. doi: 10.3892/etm.2017.4609. Epub 2017 Jun 15. Evaluation of the C-domain of heparanase during AGE-induced macrophage inflammatory response

Zhou W, Liu X, Zhang X, Tang J, Li Z, Wang Q, Hu R. Oncotarget. 2017 Jul 22;8(35):58903-58917. doi: 10.18632/oncotarget.19440. eCollection 2017 Aug 29. Oroxylin A inhibits colitis by inactivating NLRP3 inflammasome

Wang Y, Qin Y, Wang T, Chen Y, Lang X, Zheng J, Gao S, Chen S, Zhong X, Mu Y, Wu X, Zhang F, Zhao W, Zhong Z. Sci Rep. 2018 Feb 13;8(1):2887. doi: 10.1038/s41598-018-20958-1. Pyroptosis induced by enterovirus 71 and coxsackievirus B3 infection af...

El-Horany, H.E., Abd-Ellatif, R.N., Watany, M., Hafez, Y.M., Okda, H.I. (2017). NLRP3 expression and urinary HSP72 in relation to biomarkers of inflammation and oxidative stress in diabetic nephropathy patients. IUBMB Life. Advance online publicat...

Sun, G., Yang, W., Zhang, Y., & Zhao, M. (2017). Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats. Experimental Animals. Advance online publication. doi: 10.1538/expanim.16-0102

Jian, D., Dai, B., Hu, X., Yao, Q., Zhang, L., Zhu, J. (2017). ox-LDL increases microRNA-29a transcription through up-regulating YY1 and STAT1 in macrophages. Cell Biology International. Advance online publication. doi: 10.1002/cbin.10803

Huang Y, Liu L, Ma D, Liao Y, Lu Y, Huang H, Qin W, Liu X, Fang F. Human cytomegalovirus triggers the assembly of AIM2 inflammasome in THP-1-derivedmacrophages. J Med Virol. 2017 May 8. doi: 10.1002/jmv.24846. [Epub ahead of print]

Sun H, Zhu X, Cai W, Qiu L. Sun H1, Zhu X2, Cai W3, Qiu L4. Hypaphorine Attenuates Lipopolysaccharide-Induced Endothelial Inflammation via Regulation of TLR4 and PPAR-γ Dependent on PI3K/Akt/mTOR Signal Pathway.

Zhao C, Bu X, Wang W, Ma T, Ma H. Plos One. 2014 Jan 8;9(1):E85058. Doi: 10.1371/Journal.Pone.0085058. Ecollection 2014. Gec-Derived Sfrp5 Inhibits Wnt5A-Induced Macrophage Chemotaxis And Activation.

Lin H, Qu Y, Geng Z, Li C, Wu H, Dong N, Liu Z, Li W. Plos One. 2014 Jan 31;9(1):E87368. Doi: 10.1371/Journal.Pone.0087368. Ecollection 2014. Air Exposure Induced Characteristics Of Dry Eye In Conjunctival Tissue Culture.

Zhang R, Jiang F, Chen Cs, Wang T, Feng J, Tao T, Qin X. Mediators Inflamm. 2015;2015:956082. Doi: 10.1155/2015/956082. Epub 2015 May 31. Serum Levels Of Il-1 ?? , Il-6, Tgf- ?? , And Mmp-9 In Patients Undergoing Carotid Artery Stenting And Regula...

Wang Yh, Xuan Zh, Tian S, Du Gh. Evid Based Complement Alternat Med. 2015;2015:189239. Doi: 10.1155/2015/189239. Epub 2015 Feb 18. Echinacoside Protects Against 6-Hydroxydopamine-Induced Mitochondrial Dysfunction And Inflammatory Responses In Pc12...

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2 Reviews For Human IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™

0

"User Friendly And Economical ELISA Kit"

Excellent

Source: Biocompare.com

"User Friendly And Economical ELISA Kit"

--Dhruva Bhattacharya, University of Arizona, Opthalmology and Vision Science, Postdoctoral Research Associate

Application ELISA
Sample "Culture media supernatant" of transformed cultured cells of human conjunctiva epithelial cells
Detection --

"I am very much satisfied with the product Human IL-1 Beta PicoKine™ ELISA Kit (EK0392) purchased from Boster Bio. I used the Boster Bio ElISA kit for the first time and I am very happy with this product. After trying the IL-1beta kit, I have purchased two other cytokine detection ELISA kits from Boster Bio. The methodology of Human IL-1 Beta PicoKine™ ELISA Kit is simplified and user friendly. It required minimum optimization for my experiments. The written instructions provided with the kit is very useful for running the ELISA. The results obtained so far have been consistent and reproducible. For my future ELISA experiments, I'll be buying the ELISA kit from Boster Bio."

Verified customer

Submitted

0

"Good quality budget option"

Excellent

Source: Biocompare.com

"Good quality budget option"

--Milos Lazic, JECURE THERAPEUTICS, SCIENTIST II

Applications ELISA
Sample protein concentrations in cell culture media
Detection --

"We are a pharmaceutical startup optimizing cell based drug assays where one of our endpoints was observing the differences in mature IL-1b production in the media. I have compared several kits from the most expensive versions of R&D, over eBioscience/Affymetrix to a more budget Boster's kit. The aim was to decide what is the best price for the value and quality we are getting for reliable and replicable ELISA analyses. This assay was used to test the effect of increasing concentrations of a drug on IL-1b levels (dose response). The kit reported similar data obtained from the other two kits (R&D and eBiosciences), with a slightly larger standard deviations. Results replicated the findings of the previous kits and the assay had good sensitivity/ instructions on how to perform assay and analyze data. I would use this assay in an academic lab for a project where no more than 2-3 96-well plates will be used. It should be done carefully by a scientist that has previously performed ELISA assays. There are many steps to it. It is a good quality budget option to the other leading kits on the market. I would not recommend it for a larger drug screen assay where more than 5 ELISAs kits ought to be used."

Verified customer

Submitted

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17 Customer Q&As for Human IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-12-14

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-12-14

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of IL-1 beta in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

S. Harris

Verified customer

Asked: 2020-11-13

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-1 beta ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-11-13

Question

Does this ELISA recognize specifically the mature form of IL1-beta?

Verified customer

Asked: 2020-09-10

Answer

This ELISA does not recognize specifically the mature form of IL1-beta.

Boster Scientific Support

Answered: 2020-09-11

Question

Is there any cross-reactivity with the IL1 beta precursor pro-IL1 beta?

Verified customer

Asked: 2020-09-10

Answer

There is cross-reactivity with the IL1 beta precursor pro-IL1 beta.

Boster Scientific Support

Answered: 2020-09-11

Question

Do you offer an Alternative ELISA that is specific to the mature IL-1-beta?

Verified customer

Asked: 2020-09-10

Answer

We haven't got any ELISA that is specific to the mature IL-1-beta.

Boster Scientific Support

Answered: 2020-09-11

Question

Do you offer an Alternative ELISA that is specific to the mature IL-1-beta?

Verified customer

Asked: 2020-09-10

Answer

We haven't got any ELISA that is specific to the mature IL-1-beta.

Boster Scientific Support

Answered: 2020-09-11

Question

Q: if the enzyme conjugated IL-1 beta antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-1 beta antigen?

Verified Customer

Verified customer

Asked: 2019-12-18

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-12-18

Question

we received a customer inquiry regarding the products listed below. Our customer is interested in: ek0392 // ABIN411293 ek0410 // ABIN411303 ek0525 // ABIN411361 and ek0437 // ABIN411318 Do you know if your Kit has been tested for colostrum/breast milk or might work with breast milk samples as well?

Verified Customer

Verified customer

Asked: 2019-12-13

Answer

We haven't tested ek0392, ek0410 ek0525 , and ek0437 on colostrum/breast milk. We are uncertain if the kits are able to work on colostrum/breast milk. Please suggest the custome run pilot test if he wants to try. In theory, if the expression level of the targets protein in the colostrum/breast milk falls in the assay range of the kits, the kits are able to detect the protein in the sample.

Boster Scientific Support

Answered: 2019-12-13

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

D. Babu

Verified customer

Asked: 2019-09-22

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-09-22

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

T. Williams

Verified customer

Asked: 2019-05-05

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-05-05

Question

Have you tested EK0392 in tissue homogenates and Cell culture supernates?

Verified customer

Asked: 2019-03-15

Answer

The Human IL-1 beta EK0392 Elisa Kit has not been tested on tissue homogenates. Endogenous biotin in tissue homogenates might introduce a false positive signal. We recommend to run a pilot test to confirm the effect of the endogenous biotin first. Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, proceed and work on the tissue sample by using the kit.

Boster Scientific Support

Answered: 2019-03-18

Question

Q: What is the optimal O.D. value for IL-1 beta ELISA kit? I used your IL-1 beta ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain IL-1 beta even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-09-17

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-1 beta ELISA kit to have sensitivity of 0.15pg/ml, that means the minimum amount of IL-1 beta that can be declared/interpreted as positive by the above standard is 0.15pg/ml.

Boster Scientific Support

Answered: 2018-09-17

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

V. Scott

Verified customer

Asked: 2018-05-21

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-05-21

Question

For EK0392, what is the suggested dilution for plasma samples? Also, has this kit been tested for brain tissues or tissues in general. Do you recommend the customer to use brain tissue samples with this kit? suggestions?

Verified Customer

Verified customer

Asked: 2017-11-09

Answer

The suggested dilution for plasma samples is 1:1. EK0392 hasn't been tested for any tissues. Endogenous biotin in tissue homogenates might introduce false positive signal. We recommend the customer run a pilot test as described below to confirm the affect of endogeous biotin first. Add tissue homogenates into the wells and then add ABC abd TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, the customer can work on the tissue sample by using the kit.

Boster Scientific Support

Answered: 2017-11-09

Question

Hi, i want to use your ELISA kits in my study. I will use human serum in depression patients. And i want to measure IL-1beta(EK0392), BDNF(EK0307), TNF-alpha(EK0525) and Interferon-Gamma(EK0373) with BOSTER kits. Do you recommend any dilutions for my samples with these ELISA kits of yours? Or should i make my measures directly, without any dilution?

Verified Customer

Verified customer

Asked: 2015-11-10

Answer

Our lab technicans haven't woked on

Boster Scientific Support

Answered: 2015-11-10

Question

Hello Support: I need to know how I can prepare the product EK0392 diluted into 80 fold in reagent preparation that is enough for 96 well, I need the volume. Thanks!

Verified Customer

Verified customer

Asked: 2014-04-02

Answer

For the product EK0392 you will need a 100ul of antibody for each 96 well plate. So if you are going to use all the 96 well plate, you need 9600ul (9.6ml) volume of antibody. Usually there is a loss of few micro liters while pipetting, so we suggest preparing more than what it is required.

Boster Scientific Support

Answered: 2014-04-02

Question

Q: how to thaw whole blood sample for IL-1 beta ELISA after freezing?

K. Carter

Verified customer

Asked: 2014-03-11

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-1 beta for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2014-03-11

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