Mouse IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine®

IL-1 beta/IL-1F2 ELISA kit for Mouse

Mouse IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il1b in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. Cited in 10 publication(s). Independently reviewed in 1 review(s).

Product Info Summary

SKU: EK0394
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

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Product Name

Mouse IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine®

View all IL-1 beta/IL-1F2 ELISA kits

SKU/Catalog Number

EK0394

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il1b in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0394)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V118-S269

Sensitivity

<1 pg/ml

Assay Range

12.5 pg/ml - 800 pg/ml

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0394 is reactive to Il1b in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0394 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of IL-1 beta/IL-1F2

Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Kit Components

Catalog Number Description Quantity
EK0394-CAP Anti-Mouse Il1b Pre-coated 96-well strip microplate 1
EK0394-ST Mouse Il1b Standard 2 vials, 10 ng/tube
EK0394-DA Mouse Il1b Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)012.52550100200400800
O.D.0.0300.0650.1230.2120.3360.6091.0311.898

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-1 beta/IL-1F2, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..

Some articles we found to cite concentrations of IL-1 beta/IL-1F2 in samples: 23025713 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3321148336217481
Standard deviation1.7410.3334.292.1914.7535.59
CV (%)5.3%4.9%7.1%6.1%6.8%7.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-1 beta/IL-1F2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 133343132321.113.4%
Sample 22112062032082072.911.4%
Sample 348345243343044921.14.6%
*number of samples for each test n=16.

Gene/Protein Information For Il1b (Source: Uniprot.Org, NCBI)

Uniprot ID

P10749

Gene ID

16176

Gene Name

Il1b

Full Name

Interleukin-1 beta

Weight

30.931kDa

Superfamily

IL-1 family

Alternative Names

catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta Il1b|IL-, IL-1beta, Il-1b|interleukin 1 beta|interleukin-1 beta|IL-1 beta

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il1b, check out the Il1b Infographic

Il1b infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il1b: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0394 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Luteolin alleviates NLRP3 inflammasome activation and directs macrophage polarization in lipopolysaccharide-stimulated RAW264.7 cells

Increased expression of CXCR5 and CXCL13 in mice with experimental autoimmune myocarditis.

Unfractionated heparin attenuates intestinal injury in mouse model of sepsis by inhibiting heparanase

Toll-like receptor 4 implicated in acute lung injury induced by paraquat poisoning in mice

Indoxyl sulfate induces intestinal barrier injury through IRF1-DRP1 axis-mediated mitophagy impairment

The Role of Macrophage Migration Inhibitory Factor (MIF) in Asthmatic Airway Remodeling

Acacetin protects against cerebral ischemia-reperfusion injury via the NLRP3 signaling pathway

USP19 Inhibits TNF-α– and IL-1β–Triggered NF-κB Activation by Deubiquitinating TAK1

The Anti-inflammatory Effects of Heat Shock Protein 72 Involve Inhibition of High-Mobility-Group Box 1 Release and Proinflammatory Function in Macrophages

Signaling via the CXCR5/ERK pathway is mediated by CXCL13 in mice with breast cancer

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1 Reviews For Mouse IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine®

0

Easy-to-Use Cytokine Quantification--Divya Sharma, NDSU, Pharmaceutical Sciences, Graduate Student

Excellent

Source: Biocompare.com

Application ELISA
Sample Cell culture media
Detection --

"The ELISA kit from Boster Bio was easy to use and gave reproducible results each time. The protocol was well explained and each thing was paid good attention. They also provided tools for data analysis (myassay.com) which I really found useful. The standard plot was as suggested. The cytokines analyzed were comparable to literature reported. Will definitely purchase again. Excellent product." (Customer’s Tip: "Stick to the protocol.")

Verified customer

Submitted

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19 Customer Q&As for Mouse IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine®

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis IL-1 beta using the EK0394 Mouse IL-1 beta Picokine® ELISA Kit?

T. Li

Verified customer

Asked: 2020-11-21

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2020-11-21

Question

What form of the IL1-B is detected by EK0394? Will it detect both forms: before and after cleavage?

Verified customer

Asked: 2020-10-21

Answer

The Mouse IL-1 Beta ELISA Kit PicoKine™ (EK0394) detects both forms: before and after cleavage.

Boster Scientific Support

Answered: 2020-10-22

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-07-15

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-07-15

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-06-24

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-06-24

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2020-06-17

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-06-17

Question

Does this Mouse IL-1 Beta ELISA Kit PicoKine detect both pro and mature forms of IL1 beta?

Verified Customer

Verified customer

Asked: 2020-04-03

Answer

The Mouse IL-1 Beta ELISA Kit PicoKine detects only the mature form of IL-1 beta. Detection of the pro form has not been tested.

Boster Scientific Support

Answered: 2020-04-03

Question

Our customer wonder about the "Customer Q&As" of the Mouse IL-1 Beta PicoKine

Verified Customer

Verified customer

Asked: 2019-10-21

Answer

Please see the following answers for the questions. 1) Should we need to centrifuge the homogenates, collect the supernatant for detection? Please centrifuge the homogenates and collect the supernatant for detection. 2) How much of ABC and TMB should be used to mixed with the sample ? Do you have any suggestion ? Please follow the protocol specified on the kit insert without adding the detection antibody. Add 100

Boster Scientific Support

Answered: 2019-10-21

Question

Is there a need to centrifuge the homogenates in collecting supernatant for detection using EK0394 kit?

Verified customer

Asked: 2019-09-06

Answer

For the Mouse IL-1 Beta ELISA Kit PicoKine EK0394, centrifuge the homogenates and collect the supernatant for detection.

Boster Scientific Support

Answered: 2019-09-08

Question

How much of ABC and TMB should be used to mix with the sample using EK0394 kit?

Verified customer

Asked: 2019-09-06

Answer

For the Mouse IL-1 Beta ELISA Kit PicoKine EK0394, add 100 µl of the prepared 1x Avidin-Biotin-Peroxidase Complex and 90 µl of TMB Color Developing Reagent into each well.

Boster Scientific Support

Answered: 2019-09-08

Question

I would like to ask if whether this antibody Mouse IL-1 Beta ELISA Kit PicoKine is suitable for heparin or EDTA and it would not affect the ELISA procedure.

Verified Customer

Verified customer

Asked: 2019-08-23

Answer

The product Mouse IL-1 Beta ELISA Kit PicoKine is compatible with EDTA or heparin.

Boster Scientific Support

Answered: 2019-08-23

Question

Can you confirm if EK0394,EK0568,EK0527,EK0411 and EK0596 are compatible with ELISA microplate washers?

Verified customer

Asked: 2019-05-30

Answer

Our ELISA kits should be compatible for all ELISA microplate washers as long as it supports a 96 well plate.

Boster Scientific Support

Answered: 2019-05-30

Question

Can the sample diluent buffer, ABC diluent buffer and antibody diluent buffer be used interchangeably between the kit?

Verified customer

Asked: 2019-05-28

Answer

For the Mouse IL-1 Beta ELISA Kit PicoKine™ (EK0394), Mouse MCP-1 ELISA Kit PicoKine™ (EK0568), Mouse TNF Alpha ELISA Kit PicoKine™ (EK0527), Mouse IL-6 ELISA Kit PicoKine™ (EK0411) and Mouse Adiponectin ELISA Kit PicoKine™ (EK0596), the sample diluent buffer, ABC diluent buffer and antibody diluent buffer can be used interchangeably between the kit. However, the sample diluent buffer can only be used to dilute standards and samples, ABC diluent buffer is used to dilute ABC only and antibody diluent buffer is used to dilute antibody only.

Boster Scientific Support

Answered: 2019-05-29

Question

My plasma samples have been somewhat hemolyzed. Does plasma hemolysis have any effect on ELISA results? Keyword: application, protocol, troubleshooting

Verified Customer

Verified customer

Asked: 2019-03-28

Answer

There will be some unspecific staining in hemolysis samples and make the result inaccurate. We suggest you collect plasma samples again, hemolysis samples are not recommended to use.

Boster Scientific Support

Answered: 2019-03-28

Question

Q: Can IL-1 beta ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2019-03-17

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-03-17

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

M. Evans

Verified customer

Asked: 2018-11-18

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-11-18

Question

Q: how to thaw whole blood sample for IL-1 beta ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-05-09

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-1 beta for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2018-05-09

Question

Q: can you suggest the dilution ratio of serum samples for detection of IL-1 beta in Mouse plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2018-05-08

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-1 beta ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-05-08

Question

Q: how to proceed with the analysis of ELISA data? I have obtained IL-1 beta level in plasma.

Verified Customer

Verified customer

Asked: 2016-07-29

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2016-07-29

Question

Q: What is the optimal O.D. value for IL-1 beta ELISA kit? I performed your IL-1 beta ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain IL-1 beta even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2014-09-07

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-1 beta ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of IL-1 beta that can be declared/interpreted as positive by the above standard is 1pg/ml.

Boster Scientific Support

Answered: 2014-09-07

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