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Product Info Summary
| SKU: | EK0394 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
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Product info
Product Name
Mouse IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™
See all IL-1 beta/IL-1F2 products
SKU/Catalog Number
EK0394
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il1b in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Mouse IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0394)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: V118-S269
Sensitivity
<1 pg/ml
Assay Range
12.5 pg/ml - 800 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0394 is reactive to Il1b in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0394 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0394-CAP | Anti-Mouse Il1b Pre-coated 96-well strip microplate | 1 |
| EK0394-ST | Mouse Il1b Standard | 2 vials, 10 ng/tube |
| EK0394-DA | Mouse Il1b Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 12.5 | 25 | 50 | 100 | 200 | 400 | 800 |
| O.D. | 0.030 | 0.065 | 0.123 | 0.212 | 0.336 | 0.609 | 1.031 | 1.898 |
Data Example Images
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Mouse IL-1 beta PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-1 beta/IL-1F2, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..
Some articles we found to cite concentrations of IL-1 beta/IL-1F2 in samples: 23025713 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 33 | 211 | 483 | 36 | 217 | 481 |
| Standard deviation | 1.74 | 10.33 | 34.29 | 2.19 | 14.75 | 35.59 |
| CV (%) | 5.3% | 4.9% | 7.1% | 6.1% | 6.8% | 7.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-1 beta/IL-1F2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 33 | 34 | 31 | 32 | 32 | 1.11 | 3.4% |
| Sample 2 | 211 | 206 | 203 | 208 | 207 | 2.91 | 1.4% |
| Sample 3 | 483 | 452 | 433 | 430 | 449 | 21.1 | 4.6% |
Protein Target Info & Infographic
Gene/Protein Information For Il1b (Source: Uniprot.Org, NCBI)
Uniprot ID
P10749
Gene ID
16176
Gene Name
Il1b
Full Name
Interleukin-1 beta
Weight
30.931kDa
Superfamily
IL-1 family
Alternative Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta Il1b|IL-, IL-1beta, Il-1b|interleukin 1 beta|interleukin-1 beta|IL-1 beta
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Il1b, check out the Il1b Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Il1b: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Mouse IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™ (EK0394)
EK0394 has been cited in 138 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Anti-inflammatory effects of three-dimensional graphene foams cultured with microglial cells
Species: Mouse
In vivo study of immunogenicity and kinetic characteristics of a quantum dot-labelled baculovirus
Species: Mouse
Unsaturated polyurethane films grafted with enantiomeric polylysine promotes macrophage polarization to a M2 phenotype through PI3K/Akt1/mTOR axis
Species: Mouse
VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate metabolism
Species: Mouse
VSIG4 mediates transcriptional inhibition of Nlrp3 and Il-1β in macrophages
Species: Mouse
CD1d1 intrinsic signaling in macrophages controls NLRP3 inflammasome expression during inflammation
Species: Mouse
Increased Liver Localization of Lipopolysaccharides in Human and Experimental NAFLD
Species: Mouse
Melatonin alleviates cadmium‐induced liver injury by inhibiting the TXNIP‐NLRP3 inflammasome
Species: Mouse
Melatonin prevents endothelial cell pyroptosis via regulation of long noncoding RNA MEG3/miR‐223/NLRP3 axis
Species: Mouse
Wei,L.,Liu,Y.,Liu,Z.H.I.P.I.N.G.,Mao,Q.I.C.H.A.O.,Shi,N.,& Yang,J.I.A.N.F.E.I.(2021).Inhibitory Effects of Astragalus Polysaccharide on Myocardial Apoptosis Induced by Hypoxia or Reoxygenation in Rats.Indian Journal of Pharmaceutical Sciences,83(3),614-617.
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:MYOCARDIAL CELL, DILUTION:NA
Yanyan Yu,Xiangqian Li,Wenpeng Hu,et al.Self-developed NF-κB inhibitor 270 protects against LPS-induced acute kidney injury and lung injury through improving inflammation.Authorea.June 22,2021.
Species: Human,Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:PLASMA AND BMDMS, DILUTION:NA
Tawfiq,R.A.,Attia,Y.M.,Nassar,N.N.,Hammam,O.A.,Elmazar,M.M.,& Abdallah,D.M.(2021).Insights into the Impact of FXR Activation on Hepatic Autophagy in a Non-alcoholic Steatohepatitis Model.Bulletin of Pharmaceutical Sciences.Assiut,44(1),265-274.
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:LIVER TISSUE, DILUTION:NA
Lv M,Xue G,Cheng H,Meng P,Lian X,Hölscher C,Li D. The GLP-1/GIP dual-receptor agonist DA5-CH inhibits the NF-κB inflammatory pathway in the MPTP mouse model of Parkinson‘s disease more effectively than the GLP-1 single-receptor agonist NLY01. Brain Behav. 2021 Jun 14. doi: 10.1002/brb3.2231. Epub ahead of print. PMID: 34125470.
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE, DILUTION:NA
Xingwei Xiang,Xiaoling Zhou,Wenjie Wang,Yufang Zhou,Xuxia Zhou,Shanggui Deng,Bin Zheng,Zhengshun Wen,Effect of Antarctic Krill Phospholipid (KOPL) on High Fat Diet-Induced Obesity in Mice,Food Research International,2021,110456,ISSN 0963-9969,https://doi.org/10.1016/j.foodres.2021.110456.
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Zhang W,Li J,Yao H,Li T.Restoring microRNA-499-5p Protects Sepsis-Induced Lung Injury Mice Via Targeting Sox6.Nanoscale Res Lett.2021 May 21;16(1):89.doi:10.1186/s11671-021-03534-x.PMID:34019224.
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:BALF, DILUTION:NA
Li D,Weng Y,Wang G,Zhen G.Anti-Septic Potential of 7-α-Obacunyl Acetate Isolated from the Toona sinensis on Cecal Ligation/Puncture Mice via Suppression of JAK-STAT/NF-κB Signal Pathway.Infect Drug Resist.2021;14:1813-1821https://doi.org/10.2147/IDR.S302853
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Wang Y,Gao Y,Huang Y,Pan Y,Yu Y,Zhou Y,Wan SS,Yang YN.The potential protective effects of miR-497 on corneal neovascularization are mediated via macrophage through the IL-6/STAT3/VEGF signaling pathway.Int Immunopharmacol.2021 May 10;96:107745.doi:10.1016/j.intimp.2021. 107745. Epub ahead of print.PMID:33984719.
Species: Human,Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:CORNEAL TISSUE, DILUTION:NA
Ren JL,Chen Y,Zhang LS,Zhang YR,Liu SM,Yu YR,Jia MZ,Tang CS,Qi YF,Lu WW.Intermedin1-53 attenuates atherosclerotic plaque vulnerability by inhibiting CHOP-mediated apoptosis and inflammasome in macrophages.Cell Death Dis.2021 May 1;12(5):436.doi:10.1038/s41419-021-03712-w.PMID:33934111.
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:PLASMA, DILUTION:NA
Ai Z,Cheng C,Zhou L,Yin S,Wang L,Liu Y.Bone marrow mesenchymal stem cells-derived extracellular vesicles carrying microRNA-221-3p protect against ischemic stroke via ATF3.Brain Res Bull.2021 Apr 28:S0361-9230(21)00125-8.doi:10.1016/j.brainresbull.2021.04.022.Epub ahead of print.PMID:33932490.
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:BMSCS AND BRAIN TISSUE, DILUTION:NA
Yilun Luo,Xiaowen Zheng,Peiqi Yuan,Xingyao Ye,Lie Ma,Light-induced dynamic RGD pattern for sequential modulation of macrophage phenotypes,Bioactive Materials,Volume 6,Issue 11,2021,Pages 4065-4072,ISSN 2452-199X,https://doi.org/10.1016/j.bioactmat.2021.04.018.
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:BMDM, DILUTION:NA
Duan Y,Zheng H,Li Z,Yao Y,Ding J,Wang X,Nakkala JR,Zhang D,Wang Z,Zuo X,Zheng X,Ling J,Gao C.Unsaturated polyurethane films grafted with enantiomeric polylysine promotes macrophage polarization to a M2 phenotype through PI3K/Akt1/mTOR axis.Biomaterials.2020 Jul;246:120012.doi:10.1016/j.biomaterials.2020.120012.Epub 2020 Mar 31.PMID:32276198.
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:MACROPHAGES, DILUTION:NA
Zhang Y,Wang R,Shi W,Zhihui Z,Wang X,Li C,Zhang S,Zhang P. Antiviral effect of fufang yinhua jiedu (FFYH) granules against influenza A virus through regulating the inflammatory responses by TLR7/MyD88 signaling pathway.J Ethnopharmacol.2021 Apr 1:114063.doi:10. 1016/j.jep.2021.114063.Epub ahead of print.PMID:33813013.
Species: Mouse,Canine
EK0394 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Zhu Y,Liang H,Liu X,Wu J,Yang C,Wong TM, Kwan KYH,Cheung KMC,Wu S,Yeung KWK.Regulation of macrophage polarization through surface topography design to facilitate implant-to-bone osteointegration. Sci Adv.2021 Apr 2;7(14):eabf6654.doi:10. 1126/sciadv.abf6654.PMID:33811079.
Species: Mouse,Rat
EK0394 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA
Li Y, Wang N, Ma Z, Wang Y, Yuan Y, Zhong Z, Hong Y, Zhao M. Lipoxin A4 protects against paraquat‑induced acute lung injury by inhibiting the TLR4/MyD88‑mediated activation of the NF‑κB and PI3K/AKT pathways. Int J Mol Med. 2021 May;47(5):86. doi: 10.3892/ijmm. 2021.4919. Epub 2021 Mar 24. PMID: 33760150.
Species: Mouse,Rat
EK0394 usage in article: APP:ELISA, SAMPLE:BALF AND RAW264.7 CELL, DILUTION:NA
Li-Bo Jiang, Sheng-Long Ding, Wang Ding, Di-Han Su, Fang-Xue Zhang, Tai-Wei Zhang, Xiao-Fan Yin, Lan Xiao, Yu-Lin Li, Feng-Lai Yuan, Jian Dong, Injectable Sericin Based Nanocomposite Hydrogel for Multi-modal Imaging-guided Immunomodulatory Bone Regeneration,Chemical Engineering Journal,2021,129323,ISSN 1385-8947,https://doi.org/10.1016/j.cej.2021.129323.
Species: Mouse,Rat
EK0394 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA
Shen R, Yin P, Yao H, Chen L, Chang X, Li H, Hou X. Punicalin Ameliorates Cell Pyroptosis Induced by LPS/ATP Through Suppression of ROS/NLRP3 Pathway. J Inflamm Res. 2021 Mar 5;14:711-718. doi: 10.2147/JIR.S299163. PMID: 33707964; PMCID: PMC7943540.
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:J774A.1 CELL, DILUTION:NA
He Y, Peng H, Zhang H, Liu Y, Sun H. Structural characteristics and immunopotentiation activity of two polysaccharides from the petal of Crocus sativus. Int J Biol Macromol. 2021 Mar 4:S0141-8130(21)00523-7. doi: 10.1016/j.ijbiomac.2021.03. 006. Epub ahead of print. PMID: 33676979.
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA
Yin S, Li L, Tao Y, Yu J, Wei S, Liu M and Li J (2021) The Inhibitory Effect of Artesunate on Excessive Endoplasmic Reticulum Stress Alleviates Experimental Colitis in Mice. Front. Pharmacol. 12:629798. doi: 10.3389/fphar.2021.629798
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:COLON TISSUE, DILUTION:NA
Jie Cheng, Min Chen, Hui-Qi Wan, Xue-Qin Chen, Cheng-Fu Li, Ji-Xiao Zhu, Qing Liu, Guang-Hui Xu, Li-Tao Yi,Paeoniflorin exerts antidepressant-like effects through enhancing neuronal FGF-2 by microglial inactivation,Journal of Ethnopharmacology,2021,114046,ISSN 0378-8741,https://doi.org/10.1016/j.jep.2021.114046.
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:HIPPOCAMPUS TISSUE, DILUTION:NA
Liu Hui,Zhou Chuankun,Qi Dahu,Gao Yutong,Zhu Meipeng,Tao Tenghui,Sun Xuying,Xiao Jun,Inhibiting Monoacylglycerol Lipase Suppresses RANKL-Induced Osteoclastogenesis and Alleviates Ovariectomy-Induced Bone Loss.Frontiers in Cell and Developmental Biology,VOLUME=9,YEAR=2021, PAGES=544, URL=https://www.frontiersin.org/article/10.3389/fcell.2021.640867. DOI=10.3389/fcell.2021.640867,ISSN=2296-634X
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Li ZT,Liu H,Zhang WQ.NLRC3 alleviates hypoxia/reoxygenation induced inflammation in RAW264.7 cells by inhibiting K63-linked ubiquitination of TRAF6.Hepatobiliary Pancreat Dis Int.2020 Oct;19(5):455-460.doi:10.1016/j.hbpd.2020.04.003.Epub 2020 May 3.PMID:3
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Fu DN,Wang Y,Yu LJ, Liu MJ,Zhen D.Silenced long non-coding RNA activated by DNA damage elevates microRNA-495-3p to suppress atherosclerotic plaque formation via reducing Krüppel-like factor 5.Exp Cell Res.2021 Feb 23: 112519.doi:10.1016/j.yexcr.2021.11251
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Natarajan Bhaskaran,Sangeetha Jayaraman,Cheriese Quigley,Prerna Mamileti,Mahmoud Ghannoum,Aaron Weinberg,Jason Thuener,Quintin Pan,Pushpa Pandiyan bioRxiv 2021.02.15.431361;doi: https://doi.org/10.1101/2021.02.15.431361
Species: Human,Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:TONGUE TISSUE, DILUTION:NA
Zhang G,Li T,Chang X,Xing J.Long Noncoding RNA SNHG14 Promotes Ischemic Brain Injury via Regulating miR-199b/AQP4 Axis.Neurochem Res.2021 Feb 20.doi:10.1007/s11064-021-03265-6.Epub ahead of print.PMID:33609254.
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:BV2 CELL, DILUTION:NA
Liu Y,Qi Y.Vildagliptin, a CD26/DPP4 inhibitor, ameliorates bleomycin-induced pulmonary fibrosis via regulating the extracellular matrix.Int Immunopharmacol.2020 Oct;87:106774.doi:10.1016/j.intimp.2020.106774.Epub 2020 Jul 27. PMID:32731178.
Species: Human,Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:PLASMA AND LUNG TISSUE, DILUTION:NA
Liu H,Hu X,Jiang R,Cai J,Lin Q,Fan Z,Zhao P,Wang S,Zou C,Du W,Dong Z,Liu Y.CQMUH-011 Inhibits LPS-Induced Microglia Activation and Ameliorates Brain Ischemic Injury in Mice.Inflammation.2021 Feb 2.doi:10.1007/s10753-021-01420-3.Epub ahead of print.PMID:33
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:MICROGLIAL CELL AND BRAIN TISSUE, DILUTION:NA
Zou XY,Xie RR,Li W,Su CL,Chen YS,Tang H.Novel sampangine derivatives as potent inhibitors of Cu2+-mediated amyloid-β protein aggregation, oxidative stress and inflammation.Int J Biol Macromol.2021 Jan 18:S0141-8130(21)00123-9.doi:10.1016/j.ijbiomac.2021.0
Species: Mouse,Worms
EK0394 usage in article: APP:ELISA, SAMPLE:BV2 CELL, DILUTION:NA
Liu Y,Feng M,Cai J,Li S,Dai X,Shan G,Wu S.Repurposing bortezomib for choroidal neovascularization treatment via antagonizing VEGF-A and PDGF-D mediated signaling.Exp Eye Res.2021 Jan 18;204:108446.doi:10.1016/j.exer.2021.108446.Epub ahead of print.PMID:33
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:RPE TISSUE, DILUTION:NA
Sharma D,Arora S,Banerjee A,Singh J.Improved insulin sensitivity in obese-diabetic mice via chitosan Nanomicelles mediated silencing of pro-inflammatory Adipocytokines.Nanomedicine.2021 Jan 15:102357.doi:10.1016/j.nano.2020.102357.Epub ahead of print. PMI
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA
Liu Y,Wang T,Si B,Du H,Liu Y,Waqas A,Huang S,Zhao G,Chen S,Xu A.Intratracheally instillated diesel PM2.5 significantly altered the structure and composition of indigenous murine gut microbiota. EcotoxicolEnviron Saf.2021 Jan 8;210:111903.doi:10.1016/j.eco
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:COLON TISSUE, DILUTION:NA
Zheng Q,Zhang J,Zuo X,Sun J,Liang Z,Hu X,Wang Z,Li K,Song J,Ding T,Shen X,Ma Y,Li P.Photobiomodulation Promotes Neuronal Axon Regeneration After Oxidative Stress and Induces a Change in Polarization from M1 to M2 in Macrophages via Stimulation of CCL2 in
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:MACROPHAGES, DILUTION:NA
Li Y,Song W,Tong Y,Zhang X,Zhao J,Gao X,Yong J,Wang H.Isoliquiritin ameliorates depression by suppressing NLRP3-mediated pyroptosis via miRNA-27a/SYK/NF-κB axis.J Neuroinflammation.2021 Jan 5;18(1):1.doi:10.1186/s12974-020-02040-8.PMID:33402173;PMCID:PMC7
Species: Human,Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:SERUM AND HIPPOCAMPUS TISSUE, DILUTION:NA
Chen X,He Y,Zhu Y,Du J,Sun H.linc-AAM Facilitates Gene Expression Contributing to Macrophage Activation and Adaptive Immune Responses.Cell Rep.2021 Jan 5;34(1):108584.doi:10.1016/j.celrep.2020.108584.PMID:33406422.
Species: Human,Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA
Wang W,Xu RL,He P,Chen R.MAR1 suppresses inflammatory response in LPS-induced RAW 264.7 macrophages and human primary peripheral blood mononuclear cells via the SIRT1/PGC-1α/PPAR-γ pathway.J Inflamm (Lond).2021 Feb 8;18(1):8.doi:10.1186/s12950-021-00271-x
Species: Human,Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA
Long-Wei Li,Xiao-Yi Chen,Lan-Cong Liu,Yi Yang,You-Jiao Wu,Gang Chen,Zhi-Feng Zhang,Pei Luo,Oil-in-water camellia seeds oil nanoemulsions via high pressure microfluidization: Formation and evaluation,LWT,2020, 110815,ISSN 0023-6438,https://doi.org/10.1016/
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Mingyue Shen,Xianxiang Chen,Lixin Huang,Qiang Yu,Yi Chen,Jianhua Xie,Sulfated Mesona chinensis Benth polysaccharide enhance the immunomodulatory activities of cyclophosphamide-treated mice,Journal of Functional Foods,Volume 76,2021,104321,ISSN 1756-4646,h
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELLS, DILUTION:NA
J. Med.Chem. 2021, 64, 1, 768–781 Publication Date:December 1, 2020
Species: Human,Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Xinxin Zhang,Yating Qin,Xiaoning Wan et al. Rosuvastatin Inhibits Atherosclerosis Development by Improving Lipid Accumulation and Polarization Conversion of Macrophages via Regulating Autophagy, 19 November 2020,PREPRINT (Version 1) available at Research
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Wang X,Tong Y,Zhang J,Khan N,Zhang K,Bai H,Zhang Q,Chen Y.Neuroinflammation changes with periodontal inflammation status during periodontitis in wild-type mice.Oral Dis.2020 Aug 20.doi:10.1111/odi.13618.Epub ahead of print.PMID:32815656.
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:HIPPOCAMPI, DILUTION:NA
Yang L,Liu G,Fu L,Zhong W,Li X,Pan Q. DNA repair enzyme OGG1 promotes alveolar progenitor cell renewal and relieves PM2.5-induced lung injury and fibrosis. Ecotoxicol Environ Saf.2020 Dec 1;205:111283.doi: 10.1016/j.ecoenv.2020.111283. Epub 2020 Sep 22.PM
Species: Human,Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:BALF, DILUTION:NA
Kong Y,Peng Q,Lv N,Yuan J,Deng Z,Liang X,Chen S,Wang L.Paeoniflorin exerts neuroprotective effects in a transgenic mouse model of Alzheimer‘s disease via activation of adenosine A1 receptor. Neurosci Lett.2020 Jun 21;730:135016.doi:10.1016/j.neulet.
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:HIPPOCAMPUS AND CORTEX, DILUTION:NA
Gao Y,Zhou X,Zhou Y,Zhang W,Zhao L.Chrysene accelerates the proceeding of chronic obstructive pulmonary disease with the aggravation of inflammation and apoptosis in cigarette smoke exposed mice.Hum Exp Toxicol.2020 Dec 20:960327120979343.doi:10. 1177/096
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:LUNG TISSUE, DILUTION:NA
Liao H,Li Y,Zhang X,Zhao X,Zheng D,Shen D,Li R. Protective Effects of Thalidomide on High-Glucose-Induced Podocyte Injury through In Vitro Modulation of Macrophage M1/M2 Differentiation.J Immunol Res.2020 Aug 27;2020:8263598.doi:10.1155/2020/ 8263598.PMID
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:PODOCYTES, DILUTION:NA
Zhou Z,Hou J,Mo Y,Ren M,Yang G,Qu Z,Hu Y.Geniposidic acid ameliorates spatial learning and memory deficits and alleviates neuroinflammation via inhibiting HMGB-1 and downregulating TLR4/2 signaling pathway in APP/PS1 mice.Eur J Pharmacol.2020 Feb 15;869:1
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:CORTEX AND HIPPOCAMPUS TISSUE, DILUTION:NA
Li Y,Zheng D,Shen D,Zhang X,Zhao X,Liao H.Protective Effects of Two Safflower Derived Compounds, Kaempferol and Hydroxysafflor Yellow A, on Hyperglycaemic Stress-Induced Podocyte Apoptosis via Modulating of Macrophage M1/M2 Polarization.J Immunol Res.2020
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:PODOCYTES, DILUTION:NA
Zhu J, Yang LK, Chen WL, Lin W, Wang YH, Chen T. Activation of SK/KCa Channel Attenuates Spinal Cord Ischemia-Reperfusion Injury via Anti-oxidative Activity and Inhibition of Mitochondrial Dysfunction in Rabbits. Front Pharmacol. 2019 Apr 2;10:325. doi: 1
Species: Rabbit
EK0394 usage in article: APP:ELISA, SAMPLE:SPINAL CORD, DILUTION:NA
He L,Wang X,Cheng D,Xiong Z,Liu X. Ginsenoside Rg1 improves pathological damages by activating the p21‑p53‑STK pathway in ovary and Bax‑Bcl2 in the uterus in premature ovarian insufficiency mouse models. Mol Med Rep.2021 Jan;23(1):37.doi:10.3892/mmr.2020.
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:OVARY AND UTERUS TISSUE, DILUTION :NA
Zhao S, Fan Z, Hu J, Zhu Y, Lin C, Shen T, Li Z, Li K, Liu Z, Chen Y, Zhang B. The differential effects of isoflurane and sevoflurane on neonatal mice. Sci Rep. 2020 Nov 9;10(1):19345. doi: 10.1038/s41598-020-76147-6. PMID: 33168900; PMCID: PMC7652873.
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE, DILUTION:NA
Dai Z,Chen XY,An LY,Li CC,Zhao N,Yang F,You ST,Hou CZ,Li K,Jiang C,You QD,Di B,Xu LL. Development of Novel Tetrahydroquinoline Inhibitors of NLRP3 Inflammasome for Potential Treatment of DSS-Induced Mouse Colitis. J Med Chem. 2020 Dec 17.doi: 10.1021/acs.
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:THP-M CELL AND SERUM, DILUTION:NA
Zhang Z,Jiang S,Tian H,Zeng Y,He K,Lin L,Yu F. Ethyl acetate fraction from Nymphaea hybrida Peck modulates inflammatory responses in LPS-stimulated RAW 264.7 cells and acute inflammation murine models. J Ethnopharmacol. 2020 Dec 15:113698.doi:10.1016/j.je
Species: Mouse, Nymphaea hybrida
EK0394 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELLS, DILUTION:NA
Zheng B, Ying M, Xie J, Chen Y, Wang Y, Ding X, Hong J, Liao W, Yu Q. A Ganoderma atrum polysaccharide alleviated DSS-induced ulcerative colitis by protecting the apoptosis/autophagy-regulated physical barrier and the DC-related immune barrier. Food Funct
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:COLON TISSUE, DILUTION:NA
Zhou Q, Guo D, Li X, Wang Y, Ye X, Xue S, Wang X. Anti-inflammatory effects of vinpocetine in LPS-stimulated microglia via activation of AMPK. An Acad Bras Cienc. 2020 Nov 20;92(4):e20200241. doi: 10.1590/0001-3765202020200241. PMID: 33237143.
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:BV2 MICROGLIA, DILUTION:NA
Sun HJ, Xiong SP, Cao X, Cao L, Zhu MY, Wu ZY, Bian JS. Polysulfide-mediated sulfhydration of SIRT1 prevents diabetic nephropathy by suppressing phosphorylation and acetylation of p65 NF-κB and STAT3. Redox Biol. 2020 Nov 25;38:101813. doi: 10.1016/j.redo
Species: Mouse, Human
EK0394 usage in article: APP:ELISA, SAMPLE:RENAL TISSUE, DILUTION:NA
Du Y, Liu G, Zhao L, Yao R. Protective Effect of miR-204 on Doxorubicin-Induced Cardiomyocyte Injury via HMGB1. Oxid Med Cell Longev. 2020 Nov 19;2020:8819771. doi: 10.1155/2020/8819771. PMID: 33274007; PMCID: PMC7695502.
Species: Mouse, Rat
EK0394 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Huang Y, Tang J, Cai Z, Zhou K, Chang L, Bai Y, Ma Y. Prevotella Induces the Production of Th17 Cells in the Colon of Mice. J Immunol Res. 2020 Nov 1;2020:9607328. doi: 10.1155/2020/9607328. PMID: 33204736; PMCID: PMC7657696.
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:BMDC CELLS, DILUTION:NA
Cui L, Guan X, Ding W, Luo Y, Wang W, Bu W, Song J, Tan X, Sun E, Ning Q, Liu G, Jia X, Feng L. Scutellaria baicalensis Georgi polysaccharide ameliorates DSS-induced ulcerative colitis by improving intestinal barrier function and modulating gut microbiota
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMLE:COLON EXTRACTS, DILUTION:NA
Wu X, Lin L, Wu H. Ferulic acid alleviates lipopolysaccharide-induced acute lung injury through inhibiting TLR4/NF-κB signaling pathway. J Biochem Mol Toxicol. 2020 Nov 2:e22664. doi: 10.1002/jbt.22664. Epub ahead of print. PMID: 33140555.
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:BALF, DILUTION:NA
Liu H,Fan J,Zhang W,Chen Q,Zhang Y,Wu Z.OTUD4 alleviates hepatic ischemia-reperfusion injury by suppressing the K63-linked ubiquitination of TRAF6.Biochem Biophys Res Commun.2020 Mar 19;523(4):924-930. doi:10.1016/j.bbrc.2019.12.114.Epub 2020 Jan 19.PMID:
Species: Mouse
EK0394 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL AND SERUM, DILUTION:NA
Lu, Mengchen, et al. "A hydrogen peroxide responsive prodrug of Keap1-Nrf2 inhibitor for improving oral absorption and selective activation in inflammatory conditions." Redox Biology (2020): 101565.
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Oral Administration of Resveratrol Alleviates Osteoarthritis Pathology in C57BL/6J Mice Model Induced by a High-Fat Diet
Ginsenoside Rg1 improves fertility and reduces ovarian pathological damages in premature ovarian failure model of mice
Daphnetin Protects against Cerebral Ischemia/Reperfusion Injury in Mice via Inhibition of TLR4/NF-?B Signaling Pathway
Protective effect of geraniol inhibits inflammatory response, oxidative stress and apoptosis in traumatic injury of the spinal cord through modulation of NF-?B and p38 MAPK
Chinese herb cinobufagin-reduced cancer pain is associated with increased peripheral opioids by invaded CD3/4/8 lymphocytes
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LL202 protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting MAPK/AP-1 signaling
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Synergistic Effect of Compounds from a Chinese Herb: Compatibility and Dose Optimization of Compounds from N-Butanol Extract of Ipomoea stolonifera
PER1 prevents excessive innate immune response during endotoxin-induced liver injury through regulation of macrophage recruitment in mice
Chemopreventive activity of GEN-27, a genistein derivative, in colitis-associated cancer is mediated by p65-CDX2-?-catenin axis
Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition
Unfractionated heparin attenuates intestinal injury in mouse model of sepsis by inhibiting heparanase
Homocysteine Triggers Inflammatory Responses in Macrophages through Inhibiting CSE-H2S Signaling via DNA Hypermethylation of CSE Promoter
Toll-like receptor 4 implicated in acute lung injury induced by paraquat poisoning in mice
Antenatal exposure of maternal secondhand smoke (SHS) increases fetal lung expression of RAGE and induces RAGE-mediated pulmonary inflammation
The NALP3 inflammasome is involved in neurotoxic prion peptide-induced microglial activation
Effect of Gui Zhi decoction on enteric mucosal immune in mice with collagen-induced arthritis
Li J, Diao B, Guo S, Huang X, Yang C, Feng Z, Yan W, Ning Q, Zheng L, Chen Y, Wu Y. Nat Commun. 2017 Nov 6;8(1):1322. doi: 10.1038/s41467-017-01327-4. VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate met...
Jia YP, Wang K, Zhang ZJ, Tong YN, Han D, Hu CY, Li Q, Xiang Y, Mao XH, Tang B. PLoS One. 2017 Oct 9;12(10):e0186179. doi: 10.1371/journal.pone.0186179. eCollection 2017. TLR2/TLR4 activation induces Tregs and suppresses intestinal inflammation ca...
Liu X, Zhang X, Ding Y, Zhou W, Tao L, Lu P, Wang Y, Hu R. Antioxid Redox Signal. 2017 Jan 1;26(1):28-43. doi: 10.1089/ars.2015.6615. Epub 2016 Aug 9. Nuclear Factor E2-Related Factor-2 Negatively Regulates NLRP3 Inflammasome Activity by Inhibitin...
Chen S, He Y, Hu Z, Lu S, Yin X, Ma X, Lv C, Jin G. J Histochem Cytochem. 2017 Apr;65(4):241-249. doi: 10.1369/0022155417692536. Epub 2017 Feb 7. Heparanase Mediates Intestinal Inflammation and Injury in a Mouse Model of Sepsis
Zhou W, Liu X, Zhang X, Tang J, Li Z, Wang Q, Hu R. Oncotarget. 2017 Jul 22;8(35):58903-58917. doi: 10.18632/oncotarget.19440. eCollection 2017 Aug 29. Oroxylin A inhibits colitis by inactivating NLRP3 inflammasome
Lu Y, Zhang XS, Zhang ZH, Zhou XM, Gao YY, Liu GJ, Wang H, Wu LY, Li W, Hang CH. J Neuroinflammation. 2018 Mar 19;15(1):87. doi: 10.1186/s12974-018-1118-4. Peroxiredoxin 2 activates microglia by interacting with Toll-like receptor 4 after subarach...
Zhang Y, Feng J, Cui J, Yang G, Zhu X. Mol Med Rep. 2018 Apr;17(4):5805-5813. doi: 10.3892/mmr.2018.8609. Epub 2018 Feb 15. Pre-B cell leukemia transcription factor 3 induces inflammatory responses in human umbilical vein endothelial cells and mur...
Wu X, Zhang H, Qi W, Zhang Y, Li J, Li Z, Lin Y, Bai X, Liu X, Chen X, Yang H, Xu C, Zhang Y, Yang B. Cell Death Dis. 2018 Feb 7;9(2):171. doi: 10.1038/s41419-017-0257-3. Nicotine promotes atherosclerosis via ROS-NLRP3-mediated endothelial cell py...
Zhang BC, Li Z, Xu W, Xiang CH, Ma YF Am J Transl Res. 2018 Jan 15;10(1):265-273. eCollection 2018. Luteolin alleviates NLRP3 inflammasome activation and directs macrophage polarization in lipopolysaccharide-stimulated RAW264.7 cells
Du, J., Chen, X., Wang, C., & Sun, H. (2017). Pathway analysis of global gene expression change in dendritic cells induced by the polysaccharide from the roots of Actinidia eriantha. Journal of Ethnopharmacology. Advance online publication. doi: 1...
Wang, Q., Dong, X., Wang, Y., Liu, M., Sun, A., Li, N.,..., & Li, X. (2017). Adolescent escitalopram prevents the effects of maternal separation on depression- and anxiety-like behaviours and regulates the levels of inflammatory cytokines in adult...
Feng, T., Wei, S., Wang, Y., Fu, X., Shi, L., Qu, L., & Fan, X. (2017). Rhein ameliorates adenomyosis by inhibiting NF-κB and β-Catenin signaling pathway. Biomedicine & Pharmacotherapy, 94, 231-237. doi: 10.1016/j.biopha.2017.07.089
Zhang, M., Wu, X., Xu, Y., He, M., Yang, J., Li, J.,..., & Jia, J. (2017). The cystathionine β-synthase/hydrogen sulfide pathway contributes to microglia-mediated neuroinflammation following cerebral ischemia. Brain, Behavior, and Immunity. Advanc...
Dong, X.W., Jia, Y.L., Ge, L.T., Jiang, B., Jiang, J.X., Shen, J.,…, & Xie, Q.M. (2017). Soluble epoxide hydrolase inhibitor AUDA decreases bleomycin-induced pulmonary toxicity in mice by inhibiting the p38/Smad3 pathways. Toxicology, 389, 31-41. ...
Zhang W, Li X, Liu Y, Chen H, Gong J. Biomed Pharmacother. 2017 Apr 19;90:821-834. doi: 10.1016/j.biopha.2017.04.025. Activation of imidazoline I1 receptor by moxonidine regulates the progression of liver fibrosis in the Nrf2-dependent pathway.
Wang Z, Xie J, Yang Y, Zhang F, Wang S, Wu T, Shen M, Xie M. Sci Rep. 2017 Jan 17;7:40402. doi: 10.1038/srep40402. Sulfated Cyclocarya paliurus polysaccharides markedly attenuates inflammation and oxidative damage in lipopolysaccharide-treated mac...
Yang L, Ni L, Duan Q, Wang X, Chen C, Chen S, Chaugai S, Zeldin Dc, Tang Jr, Wang Dw. Prostaglandins Other Lipid Mediat. 2015 Jan-Mar;116-117:64-75. Doi: 10.1016/J.Prostaglandins.2015.01.004. Epub 2015 Feb 14. Cyp Epoxygenase 2J2 Prevents Cardiac ...
Qin Q, Niu J, Wang Z, Xu W, Qiao Z, Gu Y. Int J Mol Sci. 2012;13(7):8379-87. Doi: 10.3390/Ijms13078379. Epub 2012 Jul 5. Astragalus Membranaceus Inhibits Inflammation Via Phospho-P38 Mitogen-Activated Protein Kinase (Mapk) And Nuclear Factor (Nf)-...
Li J, Zhao L, He X, Zeng Yj, Dai Ss. Plos One. 2013;8(3):E59257. Doi: 10.1371/Journal.Pone.0059257. Epub 2013 Mar 15. Sinomenine Protects Against Lipopolysaccharide-Induced Acute Lung Injury In Mice Via Adenosine A(2A) Receptor Signaling.
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Peng J, Wang P, Ge H, Qu X, Jin X. Plos One. 2015 May 1;10(5):E0125902. Doi: 10.1371/Journal.Pone.0125902. Ecollection 2015. Effects Of Cordycepin On The Microglia-Overactivation-Induced Impairments Of Growth And Development Of Hippocampal Culture...
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1 Reviews For Mouse IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™
0
"Easy-to-Use Cytokine Quantification"
Excellent
Source: Biocompare.com
"Easy-to-Use Cytokine Quantification"
--Divya Sharma, NDSU, Pharmaceutical Sciences, Graduate Student
| Application | ELISA |
|---|---|
| Sample | Cell culture media |
| Detection | -- |
"The ELISA kit from Boster Bio was easy to use and gave reproducible results each time. The protocol was well explained and each thing was paid good attention. They also provided tools for data analysis (myassay.com) which I really found useful. The standard plot was as suggested. The cytokines analyzed were comparable to literature reported. Will definitely purchase again. Excellent product." (Customer’s Tip: "Stick to the protocol.")
Divya Sharma
Verified customer
Submitted 2019-05-21
Customer Q&As
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18 Customer Q&As for Mouse IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis IL-1 beta using the EK0394 Mouse IL-1 beta Picokine® ELISA Kit?
T. Li
Verified customer
Asked: 2020-11-21
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2020-11-21
Question
What form of the IL1-B is detected by EK0394? Will it detect both forms: before and after cleavage?
Verified customer
Asked: 2020-10-21
Answer
The Mouse IL-1 Beta ELISA Kit PicoKine™ (EK0394) detects both forms: before and after cleavage.
Boster Scientific Support
Answered: 2020-10-22
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-07-15
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-07-15
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-06-24
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-06-24
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-06-17
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-06-17
Question
Does this Mouse IL-1 Beta ELISA Kit PicoKine detect both pro and mature forms of IL1 beta?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
The Mouse IL-1 Beta ELISA Kit PicoKine detects only the mature form of IL-1 beta. Detection of the pro form has not been tested.
Boster Scientific Support
Answered: 2020-04-03
Question
Our customer wonder about the "Customer Q&As" of the Mouse IL-1 Beta PicoKine
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
Please see the following answers for the questions. 1) Should we need to centrifuge the homogenates, collect the supernatant for detection? Please centrifuge the homogenates and collect the supernatant for detection. 2) How much of ABC and TMB should be used to mixed with the sample ? Do you have any suggestion ? Please follow the protocol specified on the kit insert without adding the detection antibody. Add 100
Boster Scientific Support
Answered: 2019-10-21
Question
Is there a need to centrifuge the homogenates in collecting supernatant for detection using EK0394 kit?
Verified customer
Asked: 2019-09-06
Answer
For the Mouse IL-1 Beta ELISA Kit PicoKine EK0394, centrifuge the homogenates and collect the supernatant for detection.
Boster Scientific Support
Answered: 2019-09-08
Question
How much of ABC and TMB should be used to mix with the sample using EK0394 kit?
Verified customer
Asked: 2019-09-06
Answer
For the Mouse IL-1 Beta ELISA Kit PicoKine EK0394, add 100 µl of the prepared 1x Avidin-Biotin-Peroxidase Complex and 90 µl of TMB Color Developing Reagent into each well.
Boster Scientific Support
Answered: 2019-09-08
Question
I would like to ask if whether this antibody Mouse IL-1 Beta ELISA Kit PicoKine is suitable for heparin or EDTA and it would not affect the ELISA procedure.
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
The product Mouse IL-1 Beta ELISA Kit PicoKine is compatible with EDTA or heparin.
Boster Scientific Support
Answered: 2019-08-23
Question
Can you confirm if the EK0394 is compatible with ELISA microplate washers?
Verified customer
Asked: 2019-05-30
Answer
Our ELISA kits should be compatible for all ELISA microplate washers as long as it supports a 96 well plate.
Boster Scientific Support
Answered: 2019-05-30
Question
Can the sample diluent buffer, ABC diluent buffer and antibody diluent buffer be used interchangeably between the kit?
Verified customer
Asked: 2019-05-28
Answer
For the Mouse IL-1 Beta ELISA Kit PicoKine™ (EK0394), the sample diluent buffer, ABC diluent buffer and antibody diluent buffer can be used interchangeably between the kit. However, the sample diluent buffer can only be used to dilute standards and samples, ABC diluent buffer is used to dilute ABC only and antibody diluent buffer is used to dilute antibody only.
Boster Scientific Support
Answered: 2019-05-29
Question
Q: Can IL-1 beta ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2019-03-17
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-03-17
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
M. Evans
Verified customer
Asked: 2018-11-18
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-11-18
Question
Q: how to thaw whole blood sample for IL-1 beta ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-05-09
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-1 beta for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-05-09
Question
Q: can you suggest the dilution ratio of serum samples for detection of IL-1 beta in Mouse plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-05-08
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-1 beta ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-05-08
Question
Q: how to proceed with the analysis of ELISA data? I have obtained IL-1 beta level in plasma.
Verified Customer
Verified customer
Asked: 2016-07-29
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2016-07-29
Question
Q: What is the optimal O.D. value for IL-1 beta ELISA kit? I performed your IL-1 beta ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain IL-1 beta even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2014-09-07
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-1 beta ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of IL-1 beta that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2014-09-07
