Anti-ATR Antibody

Product Name

Anti-ATR Antibody

View all ATR Antibodies

SKU/Catalog Number

A00262

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-ATR Antibody (Catalog # A00262). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-ATR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00262)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ATR protein.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00262 is reactive to ATR in Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

301.4 kDa

Background of ATR

Ataxia Telangiectasia Mutated (ATM) and Rad 3-related protein (ATR) is a phosphatidyl-inositol kinase (PK)-related kinase which functions in response to DNA damage and repair as well as at DNA replication checkpoints during the cell cycle. ATR activates checkpoint signaling upon genotoxic stresses, such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. ATR is a member of the DNA-PK kinase family and is closely related to ATM and DNA-PK for which DNA stimulates the observed kinase activity. Chromosomal remodeling proteins have also been reported to associate with ATR complexes, including histone deacetylases (HDAC1, HDAC2 and CHD4).  ATR is known to phosphorylate BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53 which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. ATR is a nuclear protein, but can also be found in PML nuclear bodies in certain cell types. ATR is recruited to chromatin during S-phase and redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00262 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:15,000 - 1:70,000
WB: 1:1,000 - 1:5,000
This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 301 kDa in size corresponding to ATR by western blotting in the appropriate cell lysate or extract.  Lysates from HeLa, U2OS, RAW264.7 or HEK293 cells are suggested for western blotting.  ATR is ubiquitously expressed, with highest expression levels in the testes.  Note that isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.

Validation Images & Assay Conditions

See full target information ATR

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