Mouse EGF ELISA Kit PicoKine™

Mouse EGF ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Egf in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 1pg/ml. Cited in 2 publication(s).

Product Info Summary

SKU: EK0326
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Product Name

Mouse EGF ELISA Kit PicoKine™

See all Egf products

SKU/Catalog Number

EK0326

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse EGF ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Egf in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse EGF ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0326)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: N977-R1029

Sensitivity

<1 pg/ml

Assay Range

7.8 pg/ml - 500 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0326 is reactive to Egf in Mouse samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Application Guarantee

EK0326 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0326-CAP Anti-Mouse Egf Pre-coated 96-well strip microplate 1
EK0326-ST Mouse Egf Standard 2 vials, 10 ng/tube
EK0326-DA Mouse Egf Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)07.815.631.362.5125250500
O.D.0.0350.1890.2840.4620.7521.1231.6292.545

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Egf, Dilution ratio of 1:1, around 13pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse EGF ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)19632761964270
Standard deviation1.424.3417.381.535.4418.63
CV (%)7.5%6.9%8.5%%8.1%8.5%6.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Egf in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 119192018190.73.6%
Sample 263767272704.766.8%
Sample 32762562752682687.982.9%
*number of samples for each test n=16.

Gene/Protein Information For Egf (Source: Uniprot.Org, NCBI)

Uniprot ID

P01132

Gene ID

13645

Gene Name

Egf

Full Name

Pro-epidermal growth factor

Weight

133.072kDa

Alternative Names

beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; HOMG4; pro-epidermal growth factor; URG; Urogastrone Egf|AI790464|epidermal growth factor|pro-epidermal growth factor|Pro-epidermal growth factor precursor (EGF)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Egf, check out the Egf Infographic

Egf infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Egf: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0326 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Russo F, Linsalata M, Clemente C, D'Attoma B, Orlando A, Campanella G, Giotta F, Riezzo G. Bmc Cancer. 2013 Feb 4;13:56. Doi: 10.1186/1471-2407-13-56. The Effects Of Fluorouracil, Epirubicin, And Cyclophosphamide (Fec60) On The Intestinal Barrier ...

Ge C, Yu M, Petitte Jn, Zhang C. Biol Reprod. 2009 Mar;80(3):528-36. Doi: 10.1095/Biolreprod.108.072728. Epub 2008 Nov 12. Epidermal Growth Factor-Induced Proliferation Of Chicken Primordial Germ Cells: Involvement Of Calcium/Protein Kinase C And ...

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5 Customer Q&As for Mouse EGF ELISA Kit PicoKine™

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis EGF using the EK0326 Mouse EGF Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-10-19

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2018-10-19

Question

Q: can you suggest the dilution ratio of serum samples for detection of EGF in Mouse serum? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2018-07-07

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the EGF ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-07-07

Question

Q: can I use citrate plasma as samples in Mouse EGF Picokine® ELISA Kit (Catalog # EK0326)?

Verified Customer

Verified customer

Asked: 2016-09-21

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of EGF causing disruption of its protein structure. EGF may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the EGF ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2016-09-21

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2016-03-04

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2016-03-04

Question

Q: how to proceed with the analysis of ELISA data? I measured EGF level in serum.

Verified Customer

Verified customer

Asked: 2015-04-19

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2015-04-19

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