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Product Info Summary
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Anti-Chk1/CHEK1 Antibody Picoband™
Boster Bio Anti-Chk1/CHEK1 Antibody Picoband™ catalog # A01060. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Chk1/CHEK1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01060)
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
E.coli-derived human Chk1 recombinant protein (Position: M1-Q210). Human Chk1 shares 96.7% and 97.6% amino acid (aa) sequence identity with mouse and rat Chk1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A01060 is reactive to CHEK1 in Human
A01060 is guaranteed for WB Boster Guarantee
Background of Chk1
CHEK1, Cell cycle checkpoint kinase, is an enzyme that in humans is encoded by the CHEK1 gene. By fluorescence in situ hybridization, the human CHEK1 gene is mapped to 11q24, near the ATM gene at 11q23. CHEK1 is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Furthermore, CHEK1 acts to integrate signals from ATM and ATR, and is involved in monitoring meiotic recombination, a process that involves programmed DNA breaks.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Chk1 using anti-Chk1 antibody (A01060).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: SW620 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Chk1 antigen affinity purified polyclonal antibody (Catalog # A01060) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Chk1 at approximately 54KD. The expected band size for Chk1 is at 54KD.
Gene/Protein Information For CHEK1 (Source: Uniprot.Org, NCBI)
Serine/threonine-protein kinase Chk1
protein kinase superfamily
Checkpoint, S. pombe, homolog of, 1; CHEK1; CHK1 (checkpoint, S.pombe) homolog; CHK1 checkpoint homolog (S. pombe); Chk1; CHK1serine/threonine-protein kinase Chk1; EC 2.7.11; EC 22.214.171.124 CHEK1 CHK1 checkpoint kinase 1 serine/threonine-protein kinase Chk1|CHK1 checkpoint homolog|Checkpoint, S. pombe, homolog of, 1|Chk1-S|cell cycle checkpoint kinase*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CHEK1, check out the CHEK1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CHEK1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for A01060
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