Human EGF ELISA Kit PicoKine™

Human EGF ELISA Kit PicoKine™ (96 Tests). Quantitate Human EGF in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 1pg/ml. Cited in 5 publication(s).

Product Info Summary

SKU: EK0325
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Product Name

Human EGF ELISA Kit PicoKine™

See all EGF products

SKU/Catalog Number

EK0325

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human EGF ELISA Kit PicoKine™ (96 Tests). Quantitate Human EGF in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human EGF ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0325)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: N971-R1023

Sensitivity

<1 pg/ml

Assay Range

4.7 pg/ml - 300 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0325 is reactive to EGF in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Application Guarantee

EK0325 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0325-CAP Anti-Human EGF Pre-coated 96-well strip microplate 1
EK0325-ST Human EGF Standard 2 vials, 1 ng/tube
EK0325-DA Human EGF Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)04.79.418.837.575150300
O.D.0.0380.0650.1290.1910.3260.6231.2531.939

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect EGF, Dilution ratio of 1:20, concentration in serum is 800-1200pg/ml.

Some articles we found to cite concentrations of EGF in samples: 25888933, 25849850, 22581387 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human EGF ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)11761221081131
Standard deviation0.714.259.150.825.4212.31
CV (%)6.5%5.6%7.5%8.2%6.7%9.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of EGF in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 111111112110.433.9%
Sample 276767268733.314.5%
Sample 31221181281331255.714.5%
*number of samples for each test n=16.

Gene/Protein Information For EGF (Source: Uniprot.Org, NCBI)

Uniprot ID

P01133

Gene ID

1950

Gene Name

EGF

Full Name

Pro-epidermal growth factor

Weight

133.994kDa

Alternative Names

beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; HOMG4; pro-epidermal growth factor; URG; Urogastrone EGF HOMG4, URG epidermal growth factor pro-epidermal growth factor|beta-urogastrone

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on EGF, check out the EGF Infographic

EGF infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for EGF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0325 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Jin, P., Liu, J., Zhou, Q., Li, S., Liu, W., & Xi, S. (2020). Long-term treatment with arsenite activates HER1 and HER2 through upregulating EGF, TGFα, and HSP90 in a human uroepithelial cell line. Cell biology and toxicology, 36(3), 279-284.

Transdermal delivery of human epidermal growth factor facilitated by a peptide chaperon.

Effects of Pantoprazole on Systemic and Gastric Pro- and Anti-inflammatory Cytokines in Critically Ill Patients

Russo F, Linsalata M, Clemente C, D'Attoma B, Orlando A, Campanella G, Giotta F, Riezzo G. Bmc Cancer. 2013 Feb 4;13:56. Doi: 10.1186/1471-2407-13-56. The Effects Of Fluorouracil, Epirubicin, And Cyclophosphamide (Fec60) On The Intestinal Barrier ...

Ge C, Yu M, Petitte Jn, Zhang C. Biol Reprod. 2009 Mar;80(3):528-36. Doi: 10.1095/Biolreprod.108.072728. Epub 2008 Nov 12. Epidermal Growth Factor-Induced Proliferation Of Chicken Primordial Germ Cells: Involvement Of Calcium/Protein Kinase C And ...

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7 Customer Q&As for Human EGF ELISA Kit PicoKine™

Question

Are testing results for EK0325 available?

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

For testing and results, please refer to product datasheet for EK0325.

Boster Scientific Support

Answered: 2019-12-11

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2019-09-23

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-09-23

Question

Q: if the enzyme conjugated EGF antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the EGF antigen?

Verified Customer

Verified customer

Asked: 2019-09-02

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-09-02

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

S. Smith

Verified customer

Asked: 2019-02-28

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-02-28

Question

Q: can I use heparin plasma as samples in Human EGF Picokine® ELISA Kit (Catalog # EK0325)?

Verified Customer

Verified customer

Asked: 2018-09-01

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of EGF causing degradation of its protein structure. EGF may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the EGF ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-09-01

Question

Q: Can EGF ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2017-08-19

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2017-08-19

Question

Q: how do I analyze ELISA data? I measured EGF level in serum.

Verified Customer

Verified customer

Asked: 2015-05-11

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2015-05-11

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