Human EGF ELISA Kit PicoKine®

Product Name

Human EGF ELISA Kit PicoKine®

SKU/Catalog Number

EK0325

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Pathway/role: EGF (pro-epidermal growth factor) is an EGFR ligand that promotes receptor dimerization and downstream RTK signaling (e.g., MAPK/ERK and PI3K–AKT cascades; broadly relevant to proliferation/repair and oncology research contexts). Assay context: PicoKine ELISA for human matrices with on-page sensitivity <1 pg/mL and assay range 4.7–300 pg/mL; quantitative readouts are commonly validated in plate-based ELISA testing. Often interpreted alongside ERBB-family ligands such as Human NRG1 ELISA and angiogenesis markers like Human Angiopoietin-2 ELISA when characterizing growth-factor environments.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human EGF ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0325)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: N971-R1023

Capture/Detection Antibodies

The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.

Sensitivity

<1 pg/ml

Assay Range

4.7 pg/ml - 300 pg/ml

Standard Dilution Instructions

See datasheet of EK0325 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0325 is reactive to EGF in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Application Guarantee

EK0325 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of EGF

Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. EGF acts by binding with high affinity to epidermal growth factor receptor (EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. EGF results in cellular proliferation, differentiation, and survival. It also has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. EGF has strong expression in kidney, salivary gland, cerebrum, and prostate, moderate expression in trachea and thyroid, and low expression in bone marrow, heart, spleen, thymus, uterus, and colon. No expression was detected in adrenal gland, liver, lung, cerebellum, placenta, and small intestine.

Kit Components

Catalog Number	Description	Quantity
EK0325-CAP	Anti-Human EGF Pre-coated 96-well strip microplate	1
EK0325-ST	Human EGF Standard	2 vials, 1 ng/tube
EK0325-DA	Human EGF Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	4.7	9.4	18.8	37.5	75	150	300
O.D.	0.007	0.046	0.098	0.192	0.415	0.76	1.502	2.004

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect EGF, For serum, dilution ratio is 1:20 and concentration is 800-1200 pg/ml.
For plasma, dilution ratio is 1:1 and concentration is 0-6 pg/ml..

Some articles we found to cite concentrations of EGF in samples: 25888933, 25849850, 22581387 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human EGF ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

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