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Human EGF ELISA Kit PicoKine™ (96 Tests). Quantitate Human EGF in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.. Sensitivity: 1pg/ml. Cited in 5 publication(s).
|Size:||96 wells/kit, with removable strips.|
|Sample Type:||cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.|
|Sample Volume:||100μl per well|
|Assay Range:||4.7 pg/ml - 300 pg/ml|
|Product Name||Human EGF ELISA Kit PicoKine™|
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)|
|Description||Human EGF ELISA Kit PicoKine™ (96 Tests). Quantitate Human EGF in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.. Sensitivity: 1pg/ml.|
|Cite This Product||Human EGF ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0325)|
|ELISA Validation||ELISA Validation Information|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.|
|Assay Range||4.7 pg/ml - 300 pg/ml|
|Immunogen/Standard||Expression system for standard: E.coli; Immunogen sequence: N971-R1023|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96 wells/kit, with removable strips.|
|Molecular Weight||133994 MW|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for EGF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0325-CAP||Anti-Human EGF Pre-coated 96-well strip microplate||1|
|EK0325-ST||Human EGF Standard||2 vials, 1 ng/tube|
|EK0325-DA||Human EGF Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
This data is generated from a recent batch of EK0325 Human EGF ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human EGF ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human EGF ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Here are featured tools and databases that you might find useful.
|Jin, P., Liu, J., Zhou, Q., Li, S., Liu, W., & Xi, S. (2020). Long-term treatment with arsenite activates HER1 and HER2 through upregulating EGF, TGFα, and HSP90 in a human uroepithelial cell line. Cell biology and toxicology, 36(3), 279-284.||PubMed ID 31773452|
|Transdermal delivery of human epidermal growth factor facilitated by a peptide chaperon.||PubMed ID 23385091|
|Effects of Pantoprazole on Systemic and Gastric Pro- and Anti-inflammatory Cytokines in Critically Ill Patients||PubMed ID 24250536|
|Russo F, Linsalata M, Clemente C, D'Attoma B, Orlando A, Campanella G, Giotta F, Riezzo G. Bmc Cancer. 2013 Feb 4;13:56. Doi: 10.1186/1471-2407-13-56. The Effects Of Fluorouracil, Epirubicin, And Cyclophosphamide (Fec60) On The Intestinal Barrier ...||PubMed ID 23379680|
|Ge C, Yu M, Petitte Jn, Zhang C. Biol Reprod. 2009 Mar;80(3):528-36. Doi: 10.1095/Biolreprod.108.072728. Epub 2008 Nov 12. Epidermal Growth Factor-Induced Proliferation Of Chicken Primordial Germ Cells: Involvement Of Calcium/Protein Kinase C And ...||PubMed ID 19005168|
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Comprehensive info on EGF, beautifully presented.Open Infographic
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