Anti-BAFF/TNFSF13B Antibody Picoband®

Product Name

Anti-BAFF/TNFSF13B Antibody Picoband®

View all TNFSF13B Antibodies

SKU/Catalog Number

PA1541

Size

100 μg/vial

Form

Lyophilized

Description

TNFSF13B (BAFF) is a TNF-family cytokine that promotes B-cell survival and maturation via BAFF-system receptors (including TNFRSF17/BCMA), engaging canonical immune signaling such as NF-κB (clinical context: autoimmune/B-cell biology and plasma-cell malignancy biology, putative). Assay context: validated for IHC and WB; commonly contextualized with its receptor BCMA (TNFRSF17) and costimulation marker CD40 when mapping B-cell activation states.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BAFF/TNFSF13B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1541)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human BAFF, different from the related rat and mouse sequences by three amino acids.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1541 is reactive to TNFSF13B in Human, Mouse, Rat

Observed Molecular Weight

17 kDa

Calculated molecular weight

31.2 kDa

Background of TNFSF13B

BAFF was regularly detected by enzyme-linked immunosorbent assay in brain tissue lysates and in normal spinal fluid, and in astrocytes by double fluorescence microscopy. BAFF was localized in astrocytes close to BAFF-R-expressing immune cells. BAFF receptors were strongly expressed in situ in primary central nervous system (CNS) lymphomas. The TNF superfamily member B cell-activating factor (BAFF) plays an important role in humoral immunity and in autoimmune diseases, including RA.Local BAFF gene targeting inhibited proinflammatory cytokine expression, suppressed generation of plasma cells and Th17 cells, and markedly ameliorated joint pathology. The B cell activating factor BAFF (BlyS/TALL-1/zTNF4) is a tumor necrosis factor (TNF)-related ligand that promotes B cell survival and binds to three receptors (BCMA, TACI, and the recently described BAFF-R). Human BAFF was mapped to chromosome 13q32-34.4. The standard used in this kit is recombinant soluble human BAFF (A134-L295) with the molecular mass of 19.6KDa.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1541 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Positive Control

IHC: Human Tonsil Tissue

Validation Images & Assay Conditions

See full target information TNFSF13B

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