Rabbit IgG polyclonal antibody for Tumor necrosis factor ligand superfamily member 13B(TNFSF13B) detection. Tested with WB, IHC-P in Human;Mouse;Rat. Cited in 1 publication(s).
Anti-BAFF/TNFSF13B Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-BAFF/TNFSF13B Antibody
See all BAFF/BLyS/TNFSF13B primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for BAFF/TNFSF13B detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. BAFF/TNFSF13B information: Molecular Weight: 31223 MW; Subcellular Localization: Cell membrane; Single-pass type II membrane protein; Tissue Specificity: Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T- cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. Isoform 2 is expressed in many myeloid cell lines.|
|Cite This Product||Anti-BAFF/TNFSF13B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1541)|
|Similar Products From
|Anti-BAFF/TNFSF13B Antibody may replace the following items: sc 25438|sc 271198|sc 271809|sc 32773|sc 5742|sc 5743|sc 5744.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human BAFF(265-285aa RENAQISLDGDVTFFGALKLL), different from the related rat and mouse sequences by three amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For TNFSF13B (Source: Uniprot.org, NCBI)
|NCBI Gene Id||10673|
|Species Of This Entry||Human|
|Protein Name||Tumor necrosis factor ligand superfamily member 13B|
|Superfamily||tumor necrosis factor family|
|Alternative Names||BAFF/BLyS/TNFSF13B|ApoL related ligand TALL-1; B lymphocyte stimulator; BAFF; BAFFB-cell activating factor; B-cell-activating factor; BLyS; BLYSB-lymphocyte stimulator; CD257 antigen; CD257; Dendritic cell-derived TNF-like molecule; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF13B; TNFSF20; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand superfamily member 13B; tumor n|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Citrullination; Glycosylation; Dephosphorylation; Methylation|
|Subcellular Localization||Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor ligand superfamily member 13b, soluble form]: Secreted.|
|Gene Location||13q33.3, on Chromasome 13, gene sequence: NC_000013.11 (108268240..108308484)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For TNFSF13B (Source: Uniprot.org, NCBI)
|Protein Function||Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.|
|Tissue Specificity||Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T- cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. Isoform 2 is expressed in many myeloid cell lines.|
|Interacting Proteins||CSE1L; KPNB1; TNFRSF13B|
|Related Diseases||Autoimmune Reaction; Autoimmune Diseases; Lupus Erythematosus, Systemic; Autoimmunity; Arthritis; Rheumatoid Arthritis; Malignant Neoplasms; Sjogren's Syndrome; Neoplasms; Lymphoma; Inflammation; B-cell Lymphomas; Malignant Paraganglionic Neoplasm; Multiple Myeloma; Sclerosis; Immunologic Deficiency Syndromes; Leukemia; Multiple Sclerosis; Salivary Gland Diseases; Chronic Lymphocytic Leukemia|
|Related Pathways||Pathogenesis; Cell Activation; Cell Maturation; Secretion; B Cell Activation; Cell Proliferation; Cell Development; B Cell Proliferation; Immune Response; B Cell Homeostasis; Cell Growth; Immunoglobulin Production; T Cell Activation; Cell Death; Cell Differentiation; Cell Cycle; Lymphocyte Proliferation; B Cell Differentiation; Germinal Center Formation; Localization|
|Background||BAFF was regularly detected by enzyme-linked immunosorbent assay in brain tissue lysates and in normal spinal fluid, and in astrocytes by double fluorescence microscopy. BAFF was localized in astrocytes close to BAFF-R-expressing immune cells. BAFF receptors were strongly expressed in situ in primary central nervous system(CNS) lymphomas. The TNF superfamily member B cell-activating factor(BAFF) plays an important role in humoral immunity and in autoimmune diseases, including RA.Local BAFF gene targeting inhibited proinflammatory cytokine expression, suppressed generation of plasma cells and Th17 cells, and markedly ameliorated joint pathology. The B cell activating factor BAFF(BlyS/TALL-1/zTNF4) is a tumor necrosis factor(TNF)-related ligand that promotes B cell survival and binds to three receptors(BCMA, TACI, and the recently described BAFF-R). Human BAFF was mapped to chromosome 13q32-34.4. The standard used in this kit is recombinant soluble human BAFF(A134-L295) with the molecular mass of 19.6KDa.|
|Scientific References||PMID: 10331498 by Shu H.-B., et al. TALL-1 is a novel member of the TNF family that is down-regulated by mitogens.|
PMID: 10359578 by Schneider P., et al. BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth.
Our Boster Quality Guarantee for Anti-BAFF/TNFSF13B Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Recombinant Human BAFF Protein 10ng
Lane 2: Recombinant Human BAFF Protein 5ng
Lane 3: Recombinant Human BAFF Protein 2.5ng
IHC(P): Human Tonsil Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Order Product (PA1541)
Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet.See Details
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Guaranteed product quality
We promise all of our products perform as described in datasheets.