Human TNFRSF17/BCMA ELISA Kit PicoKine™

Human TNFRSF17/BCMA ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFRSF17 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK0661
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human TNFRSF17/BCMA ELISA Kit PicoKine™

See all BCMA/TNFRSF17 products

SKU/Catalog Number

EK0661

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human TNFRSF17/BCMA ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFRSF17 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human TNFRSF17/BCMA ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0661)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-A54

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0661 is reactive to TNFRSF17 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0661 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0661-CAP Anti-Human TNFRSF17 Pre-coated 96-well strip microplate 1
EK0661-ST Human TNFRSF17 Standard 2 vials, 10 ng/tube
EK0661-DA Human TNFRSF17 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0110.1540.2560.4520.7341.2251.6781.984

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect BCMA/TNFRSF17, Dilution ratio of 1:1, concentration in serum is around 800pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human TNFRSF17/BCMA ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)6423791768247851
Standard deviation4.0913.9842.185.5716.5443.4
CV (%)6.4%4.6%4.6%8.2%6.7%5.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of BCMA/TNFRSF17 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 164586062612.233.6%
Sample 22372132212312259.24%
Sample 391784091682187343.524.9%
*number of samples for each test n=16.

Gene/Protein Information For TNFRSF17 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q02223

Gene ID

608

Gene Name

TNFRSF17

Full Name

Tumor necrosis factor receptor superfamily member 17

Weight

20.165kDa

Alternative Names

B cell maturation antigen; B-cell maturation protein; BCMA; BCMAtumor necrosis factor receptor superfamily member 17; BCMB-cell maturation factor; CD269 antigen; CD269; TNFRSF13A; TNFRSF17; tumor necrosis factor receptor superfamily, member 17 TNFRSF17 BCM, BCMA, CD269, TNFRSF13A TNF receptor superfamily member 17 tumor necrosis factor receptor superfamily member 17|B cell maturation antigen|B-cell maturation factor|B-cell maturation protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TNFRSF17, check out the TNFRSF17 Infographic

TNFRSF17 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TNFRSF17: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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11 Customer Q&As for Human TNFRSF17/BCMA ELISA Kit PicoKine™

Question

Can EK0661 detect only free BCMA or bound BCMA as well?

Verified customer

Asked: 2020-10-09

Answer

The Human TNFRSF17/BCMA ELISA Kit PicoKine™ (EK0661) detects free BCMA and bound BCMA.

Boster Scientific Support

Answered: 2020-10-12

Question

Q: how to analyze ELISA data? I have performed BCMA level in plasma.

R. King

Verified customer

Asked: 2020-08-03

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-08-03

Question

Q: which procedure should I follow in order to thaw whole blood sample for BCMA ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-06-02

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test BCMA for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-06-02

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

M. Jackson

Verified customer

Asked: 2020-03-25

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-03-25

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-08-18

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-08-18

Question

Is the standard protein provided with the EK0661 Kit tagged? Can you also confirm that the standard protein is aa 1-54?

Verified customer

Asked: 2019-07-12

Answer

The standard protein provided with the Human TNFRSF17/BCMA ELISA Kit PicoKine™ (EK0661) isn't tagged. The standard protein is aa 1-54.

Boster Scientific Support

Answered: 2019-07-15

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2019-04-09

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-04-09

Question

Hi Boster: I want to know if your EK0661 Human TNFRSF17/BCMA ELISA Kit antibody is cynomolgus cross-reactive?

Verified Customer

Verified customer

Asked: 2018-02-15

Answer

The EK0661 Human TNFRSF17/BCMA ELISA Kit antibody has never been tested with samples from cynomolgus monkey. However, all tested species cross-reactivity information is listed on the datasheets.

Boster Scientific Support

Answered: 2018-02-15

Question

Is the standard protein provided with the EK0661 kit tagged?

Verified Customer

Verified customer

Asked: 2018-01-22

Answer

The standard protein in EK0661 is not tagged.

Boster Scientific Support

Answered: 2018-01-22

Question

Could you tell me where this EK0661 antibody binds to BCMA? The intracellular domain or extracellular domain?

Verified Customer

Verified customer

Asked: 2017-11-30

Answer

The EK0661 antibody binds the intracellular domain since the immunogen is from the C-terminal region of BCMA

Boster Scientific Support

Answered: 2017-11-30

Question

Q: is it okay to use citrate plasma as samples in Human BCMA Picokine® ELISA Kit (Catalog # EK0661)?

Verified Customer

Verified customer

Asked: 2017-07-27

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of BCMA causing degradation of its protein structure. BCMA may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the BCMA ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2017-07-27

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