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Product Info Summary
| SKU: | A01014-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB, ELISA (Cap) |
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Product info
Product Name
Anti-BCMA/TNFRSF17 Antibody Picoband®
SKU/Catalog Number
A01014-1
Size
100 μg/vial
Form
Lyophilized
Description
TNFRSF17 (BCMA) is a TNF-receptor superfamily member preferentially expressed in mature B cells/plasma cells; it binds BAFF and can activate NF-κB and MAPK8/JNK via TRAF interactions (immune survival/proliferation signaling; clinical context: plasma-cell biology and myeloma target biology, putative). Assay context: validated for ELISA, IHC, and WB; naturally paired with its ligand BAFF (TNFSF13B) and costimulation receptor CD40 when mapping B-cell activation and differentiation pathways.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BCMA/TNFRSF17 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01014-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human BCMA recombinant protein (Position: M1-A54).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01014-1 is reactive to TNFRSF17 in Human
Observed Molecular Weight
20 kDa
Calculated molecular weight
20.2 kDa
Background of TNFRSF17
B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01014-1 is guaranteed for IHC, WB, ELISA (Cap) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA (Cap), 1-5μg/ml
Positive Control
WB: human Raji whole cell, human Jurkat whole cell
IHC: human tonsil tissue
Validation Images & Assay Conditions
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Western blot analysis of BCMA using anti-BCMA antibody (A01014-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human Jurkat whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCMA antigen affinity purified polyclonal antibody (Catalog # A01014-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCMA at approximately 20KD. The expected band size for BCMA is at 20KD.
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IHC analysis of BCMA using anti-BCMA antibody (A01014-1).
BCMA was detected in paraffin-embedded section of human tonsil tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-BCMA Antibody (A01014-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Sandwich ELISA - Recombinant human BCMA/TNFRSF17 protein standard curve.
Use in combination with reagents from Human BCMA/TNFRSF17 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0661).
Specific Publications For Anti-BCMA/TNFRSF17 Antibody Picoband® (A01014-1)
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1 Customer Q&As for Anti-BCMA/TNFRSF17 Antibody Picoband®
Question
How was A01014-1 purified and how was the antigen attached to the purification column?
Verified customer
Asked: 2020-09-08
Answer
Purification method of the Anti-BCMA/TNFRSF17 Antibody Picoband™ (A01014-1) was antigen affinity purified. Antigen was immobilized on a solid phase. Wash off unstable antigen. Stable antigen would bind with anti-sera.
Boster Scientific Support
Answered: 2020-09-09
