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BAX antibody (A00183) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 322 Publications

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a00183 bax primary antibodies fcm testing 6

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Flow Cytometry analysis of THP-1 cells using anti-Bax antibody (A00183).
Overlay histogram showing THP-1 cells stained with A00183 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Bax Antibody (A00183, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-Bax Antibody Picoband®

Name: Anti-Bax Antibody Picoband®
Brand: Boster Bio
SKU: A00183
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

BAX antibody

322 Publications|

1 Review

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Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	Flow Cytometry, IF, IHC, ICC, WB

Tested Applications

BAX antibody was positively detected by WB in:

human THP-1 whole cell, human MCF-7 whole cell, human A549 whole cell, human Hela whole cell, human Hela- WT whole cell, human Hela-BAX KO whole cell, rat C6 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

BAX antibody was positively detected by IHC in:

human lung cancer tissue, mouse kidney tissue, rat kidney tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

BAX antibody was positively detected by ICC/IF in:

A549 cell

BAX antibody was positively detected by IF in:

mouse uterus tissue

BAX antibody was positively detected by FC in:

THP-1 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Monkey

Predicted

Bovine

Predicted

Sheep

Predicted

Dog

Predicted

Product Information

Product Name

Anti-Bax Antibody Picoband®
View all BAX Antibodies

Form

Lyophilized

Uniprot

Q07812

Gene ID

581

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Bax, different from the related mouse and rat sequences by five amino acids.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

21 kDa

Calculated Molecular Weight

21.2 kDa

Background of BAX

Apoptosis regulator BAX, also known as bcl-2-like protein 4, is a protein that in humans is encoded by the BAX gene. The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. Additionally, this protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.

Cite This Product

Anti-Bax Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00183)

Protocols

Western Blot protocol for BAX antibody

Review Protocol

Immunohistochemistry (IHC) protocol for BAX antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for BAX antibody

Review Protocol

Immunofluorescence (IF) protocol for BAX antibody

Review Protocol

Flow Cytometry (FC) protocol for BAX antibody

Review Protocol

Publications

Effect of Sun’s Bushen Huayu formula on M1/M2-associated markers and endometrial repair in a thin endometrium model

Published: 2026-08-17

Journal: Scientific Reports

Application: WB

Impact Factor: 4.90

Species: Rat

PMID: 42680819

SKU	A00183
Application	Western Blot
Sample	mouse lung tissue
Sample Processing Description	Total protein was extracted from the lung tissues of three normal mice.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody	Bax Antibody Picoband®
Primary Incubation	1:2000, overnight at 4 ℃
Secondary Antibody	HRP-conjugated goat anti-rabbit IgG
Secondary Incubation	1:10000, 1 hour in RT
Detection	Substrate: ECL substrate; Image system: ChemiDoc MP
Results Summary	BAX is a key effector in the regulation of cell fate, acting as a “final executor” of apoptosis. Upon receiving irreversible death signals, it undergoes conformational rearrangement and oligomerization to form pores in the mitochondrial outer membrane, leading to collapse of cellular energy homeostasis and activation of the apoptotic cascade. In this study, lung tissues from three normal mice were used to evaluate the performance of the Anti-BAX antibody. The results showed a clear target band at the expected molecular weight, indicating that the antibody performs well in Western blot.

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Anti-Bax Antibody Picoband®

BAX antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Effect of Sun’s Bushen Huayu formula on M1/M2-associated markers and endometrial repair in a thin endometrium model

Prolonged sevoflurane exposure induces exaggerated and persistent cognitive decline in old rats with pre-existing impairment by increasing hippocampal mitochondrial dysfunction and apoptosis

Matrine suppresses thymoma stemness and apoptosis via YTH N6-methyladenosine RNA binding protein 1 and Wnt/β-catenin signaling

αB-Crystallin Protects Against Cisplatin-Induced Nephrotoxicity by Modulating Apoptosis In Vivo and In Vitro

Wulingsan alleviates cisplatin-induced acute kidney injury and inhibits renal tubular epithelial cell apoptosis in association with the CaSR/CaMKKβ/AMPK pathway

Unexpected Promotion of Bone Regeneration by Inhibition of BMPR1A-Mediated BMP Signalling

Effects of Ventilation During Warm Ischemia in a Rat Lung Perfusion Model

A novel role for decadienyl-L-carnitine in pulmonary vascular remodeling and the underlying interventional mechanism of osthole

Age- and diet-dependent progression of retinal microvascular injury in GCK-MODY under metabolic stress

Complement protein C1q induces endothelial apoptosis through C1qBP regulation of mitochondrial function in lung endothelial cells in systemic sclerosis

Altered Magnesium Environments Restrict Colorectal HT-29 Spheroid Growth by Disturbing Cellular Mg2+ Homeostasis

Hemp seed fiber supplementation alleviates myocardial hypertrophy in aging mice through activation of mitophagy

Silencing Uracil-DNA glycosylase inhibits colorectal cancer progression

L-tetrahydropalmatine attenuates methamphetamine-induced pulmonary vascular remodeling via CaSR-BMP2/mTOR signaling

Trimethyl Chitosan-Engineered Cod Skin Peptide Nanosystems Alleviate Behavioral and Cognitive Deficits in D-Galactose-Induced Alzheimer’s Disease Model Mice

Ion channels and atrial fibrillation: mitophagy as a key mediator

Unveiling Xuanshen decoction: A novel approach to combat slow transit constipation

Effects of Diethylstilbestrol on Uterus Structure and Immunological Function in Mice During Early Pregnancy

The Combination of Celastrol and Curcumin Enhances the Antitumor Effect in Nasopharyngeal Carcinoma by Inducing Ferroptosis

Construction of ROS-responsive cascade-targeted liposomes and investigation of their anti-AD effects

Smart biodegradable hydrogels incorporating a photoactive Ir(III) complex for NIR-triggered oral cancer treatment

Luteolin alleviates right ventricular hypertrophy in high altitude pulmonary hypertension rats by regulating PI3K/AKT/mTOR signalling pathway

GGT and GSH-triggered nanoplatform for efficient gambogic acid delivery and tumor penetration in triple-negative breast cancer

Cordycepin promotes the survival of random-pattern skin flaps via autophagy in a rat model

Protective Effect of FABP5 Against 6-OHDA Induced Parkinson’s Disease Via PPARγ/SIRT1/PGC-1α Signaling Pathway

Multi-omics analysis reveals classic decoction alleviates ulcerative colitis by modulating tryptophan metabolism, gut microbiota homeostasis, and mitochondrial Bax/Bcl-2 pathways

Downregulation of GFPT2 enhances cisplatin chemotherapy sensitivity in STK11/KRAS mutant non-small cell lung cancer by regulating the hexosamine biosynthesis pathway, resisting tumor growth

A novel peptide RR-171 derived from human umbilical cord serum induces apoptosis and pyroptosis in pancreatic cancer cells

Aloperine treatment attenuates acute spinal cord injury by reducing oxidative, inflammatory, and apoptotic responses via PI3K/AKT/NF-κB signaling in a rat contusion model

miR-107 suppresses porcine granulosa cell proliferation and estradiol synthesis while promoting apoptosis via targeting PTGS2

microRNA-30c attenuates contrast-induced acute kidney injury by reducing renal tubular epithelial cell apoptosis via targeting SOCS1

A novel identified epithelial ligand-receptor-associated gene signature highlights POPDC3 as a potential therapy target for non-small cell lung cancer

Efficacy assessment of glycyrrhetinic acid-modified liposomes loaded with doxorubicin hydrochloride and cucurbitine B for synergistic treatment of hepatocellular carcinoma

Preconditioning with acteoside ameliorates myocardial ischemia‑reperfusion injury by targeting HSP90AA1 and the PI3K/Akt signaling pathway

Downregulation of LncRNA CCAT1 Enhances Chemosensitivity in Cisplatin-Resistant Gastric Cancer Cells

High expression of ARPC1B promotes the proliferation and apoptosis of clear cell renal cell carcinoma cells, leading to a poor prognosis

Polyphenols From Morchella sextelata Induce Apoptosis of Colorectal Cancer Cells Through ROS-Mediated Endogenous Mitochondrial Apoptosis Pathway In Vitro

LncRNA SERPINB9P1 Mitigates Cerebral Injury Induced by Oxygen‒Glucose Deprivation/Reoxygenation by Interacting with HSPA2

Halcinonide activates smoothened to ameliorate ischemic stroke injury

Zhishi Xiebai Guizhi Decoction modulates hypoxia and lipid toxicity to alleviate pulmonary vascular remodeling of pulmonary hypertension in rats

Bufadienolides from Chansu Injection Synergistically Enhances the Antitumor Effect of Erlotinib by Inhibiting the KRAS Pathway in Pancreatic Cancer

Astragaloside IV plays a neuroprotective role by promoting PPARγ in cerebral ischemia-reperfusion rats

Rosmarinic acid attenuates glioblastoma cells and spheroids’ growth and EMT/stem-like state by PTEN/PI3K/AKT downregulation and ERK-induced apoptosis.

Cerium oxide nanoparticles (nanoceria) pretreatment attenuates cell death in the hippocampus and cognitive dysfunction due to repeated isoflurane anesthesia in newborn rats.

Delay the progression of glucocorticoid-induced osteoporosis: Fraxin targets ferroptosis via the Nrf2/GPX4 pathway

Brown adipose tissue-derived FGF21 mediates the cardioprotection of dexmedetomidine in myocardial ischemia/reperfusion injury

Effect of maternal cigarette smoke exposure on COPD progression in offspring mice

Regulatory role of oxidative stress in retrorsine – Induced apoptosis and autophagy in primary rat hepatocytes

Circ_0002331 Interacts with ELAVL1 to Improve ox-LDL-Induced Vascular Endothelial Cell Dysfunction via Regulating CCND2 mRNA Stability

The protective effect of Macrostemonoside T from Allium macrostemon Bunge against Isoproterenol-Induced myocardial injury via the PI3K/Akt/mTOR signaling pathway

Mitochondrial Fission in Nickel Nanoparticle-Induced Reproductive Toxicity: An In Vitro GC-1 Cell Study

Cytisine–N-methylene-(5,7,4’-trihydroxy)- isoflavone ameliorates ischemic stroke-induced brain injury in mouse by regulating the oxidative stress and BDNF-Trkb/Akt pathway

Declined circular RNA mitofusin 2 constrains the deterioration of Wilms tumor via modulating microRNA-372-3p/transforming growth factor-β receptor type 2 axis

Adiponectin attenuates H2O2-induced apoptosis in chicken skeletal myoblasts through the lysosomal-mitochondrial axis

Engineered exosomes loaded with M1–8 peptide for targeted therapy of hepatocellular carcinoma

Rosavin Alleviates LPS-Induced Acute Lung Injure by Modulating the TLR-4/NF-κB/MAPK Singnaling Pathways

Hepatitis E virus causes apoptosis of ovarian cells in hens and resulting in a decrease in egg production

Suppression of NLRP3 inflammasome activation by astragaloside IV via promotion of mitophagy to ameliorate radiation-induced renal injury in mice

Borneol-Modified Schisandrin B Micelles Cross the Blood–Brain Barrier To Treat Alzheimer’s Disease in Aged Mice

PANoptosis-like death in acute-on-chronic liver failure injury

FAXDC2 inhibits the proliferation and invasion of human liver cancer HepG2 cells

Structural identification of an polysaccharide isolated from Epimedium brevicornum and its beneficial effect on promoting osteogenesis in osteoblasts induced by high glucose

Co-exposure to polystyrene microplastics and microcystin-LR aggravated male reproductive toxicity in mice

Water Extract of Rice False Smut Balls Activates Nrf2/HO-1 and Apoptosis Pathways, Causing Liver Injury

Exosomal circCNOT6L Regulates Astrocyte Apoptotic Signals Induced by Hypoxia Exposure Through miR99a-5p/SERPINE1 and Alleviates Ischemic Stroke Injury

Deciphering anti–benign prostatic hyperplasia potential of liangwanoside II based on metabolite profile characterization combined with targeted network pharmacology

Comparison of acute kidney injury following brain death between male and female rats