Apoptosis regulator BAX Antibodies

Apoptosis regulator BAX (BAX)

Plays a role in the mitochondrial apoptotic process. Under ordinary conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria into the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of poisonous BAX levels in the mitochondrial outer membrane (MOM) (PubMed:21458670).

Under stress conditions, undergoes a conformation change that leads to translocation into the mitochondrion membrane, resulting in the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

Gene Information

Human
Gene Name:	BAX
Uniprot:	Q07812
Entrez:	581

Family

Belongs to:
Bcl-2 family

Alternative Names

apoptosis regulator BAX; Bax; BCL2-associated X protein; Bcl2-L-4; BCL2L4bcl2-L-4; Bcl-2-like protein 4

Molecular Weight

Mass (kDA):
21.184 kDA

Genomic Context

Human
Location:	19q13.33
Sequence:	19; NC_000019.10 (48954825..48961798)

Tissue Specificity

Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro- myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T- cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.

Subcellular Localizations

[Isoform Alpha]: Mitochondrion outer membrane; Single-pass membrane protein. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.; [Isoform Beta]: Cytoplasm.; [Isoform Gamma]: Cytoplasm.; [Isoform Delta]: Cytoplasm.

Diseases

• Malignant Neoplasms
• Neoplasms
• Carcinoma
• Leukemia
• Malignant Neoplasm Of Breast
• Mammary Neoplasms
• Malignant Paraganglionic Neoplasm
• Ischemia
• Adenocarcinoma
• Liver Carcinoma
• Inflammation
• Malignant Tumor Of Colon

• Lymphoma
• Cell Transformation, Neoplastic
• Malignant Neoplasm Of Prostate
• Malignant Neoplasm Of Lung
• Prostatic Neoplasms
• Malignant Squamous Cell Neoplasm
• Lung Neoplasms

Interacting Proteins

• BAK1
• BCL2
• BCL2L1
• BCL2L10
• BCL2L11

• BCL2L2
• BID
• Bid
• ERN1
• Gimap3

• Gimap5
• KCNA3
• MCL1
• Mcl1
• Mfn1

• Mfn2
• MT-RNR2
• PYCARD
• RNF144B
• RTL10

• UVRAG
• XRCC6

Pathways

• Cell Death
• Cell Cycle
• Induction Of Apoptosis
• Cell Proliferation
• Cell Growth
• Cell Cycle Arrest
• Programmed Cell Death
• Pathogenesis
• Localization
• Apoptotic Process
• Angiogenesis
• Neuroprotection
• Release Of Cytochrome C From Mitochondria

• Autophagy
• S Phase
• Drug Resistance
• Mitochondrial Translocation
• Secretion
• Aging
• Rna Interference

PTMs

• Cleavage
• Phosphorylation
• Acetylation
• Dephosphorylation
• Reduction
• Oxidation
• Ubiquitination
• Methylation
• Deacetylation
• Glycosylation

• Nitration
• Phosphorothioation
• Demethylation
• Prenylation
• Biotinylation
• Ribosylation
• Farnesylation
• Myristoylation
• Deamidation
• Sumoylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/BAX

Boster Bio: Best Uses Of The BAX Marker

The Boster Bio: Best Uses of the Bayx Marker is an incredibly versatile DNA and protein microarray marker. Its wide array of applications enables scientists to submit results for species, applications, and special samples. In addition, scientists are able to get product credits for their work. The BAX marker is universally applicable to scientists all over the world. In addition, it's highly compatible with other protein microarray and DNA markers including those from other brands.

p23

If you're looking to conduct DNA manipulation experiments, you might be interested in the p23 marker in Boster Bio: Best Practices For Molecular Biology. The BAX marker is used to identify the presence of genes whose expression alters the function of the mitochondrial outer membrane. BAX is cytosolic under normal conditions. Its continuous retrotranslocation stops accumulation within the outer membrane. When it is subjected to stress, BAX undergoes a conformational change and is transferred to the mitochondrion membrane, which initiates Apoptosis. It also stimulates the activation of CASP3 which is a gene that plays a role in cellular death.

The Boster Bio Anti-Bax (Apoptosis Marker) Monoclonal Antibody reacts with Human, Mouse, Rat and Mice. It was designed specifically for these species and comes with numerous assays to validate. This antibody reacts with human BAX's N-terminal adduct, unlike other antibodies. This makes it appropriate for a range of apoptosis treatments.

TUSC3

You have come to the right place if you are looking for the best Tusc3 agents. CUSABIO manufactures Tusc3 using strict quality control and validates every product to be used in a variety of ways. These reagents are backed by solid technical support to ensure that you benefit from your research. Find out more about the advantages of Tusc3 reagents from Boster Bio.

Boster Bio Anti N33 TUSC3 antibody was examined against mouse, human and rat tissues. It is highly affinity and binds to the protein in a variety of ways. Its highly specific binding properties make it a perfect TUSC3 antibody. It is available in various formats, including IHC or ELISA. Customers can request a validation process or image for each kit. Boster Bio Anti-N33 TUSC3 ELISA Kit is available in two- and four-color versions. One ELISA detects mouse, human and the rat TUSC3.

Reverse transcription-PCR (RTPCR) was used to get the combined MagT1 and TUSC3 cDNAs from HEK-293T cells the mRNA. The cDNAs were subcloned into the pcDNA3.1. After the cloning process, proteins could be expressed in a way which caused instability of C-terminal tag expression. GFP and HA tags were added to the signal peptides. Additionally an TM3-4 loop was inserted between residues 290 and 291 of MagT1 as well as the TUSC3 protein, resulting in proteins with subcellular location similar to those of untagged proteins.

p23 may sustain the folding of Bax

The heat shock protein Hsp90, or p23, is a cochaperone that stabilizes the Bax-Hsp90 heterocomplex in mammals, yeast and orchardgrass. This orchardgrass protein p23 (also known as Dgp23) is a 911 bp DNA with 180 amino acids. It has an open reading frame that is heat-inducible. Recombinant Dgp23, a small acidic protein, has a molecular weight of 27 kD, with a high pI of 4.3.

The p23 might be an obligated partner for another function of Bax

There are many reasons for the lower expression of p23 in Bak MEFs. One reason is that endogenous Bax does not downshift into fractions containing transmutant Bax. The absence of p23 did not result in an increase in UVor etoposide-induced cell death. Furthermore, the absence of the p23 protein in Bax-/MEFs may be due to the possibility that p23 is not the main sequestering protein of cytosolic Bax. However the observed differences in the pattern of migration aren't related to the presence of p23. They could be the result of differences in posttranslational modifications or structural features.

Bcl-2, a protein that has conserved domains, plays a role in the process of apoptosis. It is involved in homodimerization of Raf-1 Kinase. It also regulates cell death. Bcl-2 has a dual function acting as a mediator of cell death through apoptosis and R-ras.

The activation processes of BAX are an intricate process. It is interesting to note that even a tiny variation in the expression of BAX can affect cell death. Recent research suggests that the BCL2 protein family plays an important role in an anti-apoptotic switching mechanism. In order for a cell to undergo apoptosis, it must possess two concentration-dependent components: sufficient levels of antagonizing BH3-only molecules, and sufficient levels of BAX. In order to sequester BCL2 family proteins, BAX must be expressed in sufficient quantities.

Storage of the BAX Marker

The cell membrane biochemistry is dependent on the storage of BAX markers. Bax and Bak are activated by inserting the a9 Helix from Bax into the membrane. Then the BH3 domain is exposed and the protein self-assembled with other Bax or Bak molecules. Once this activation mechanism is complete the protein can be again folded and released.