Click image to see more details
Product Info Summary
| SKU: | M01571 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-AIF AIFM1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01571
BM3930 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-AIF AIFM1 Rabbit Monoclonal Antibody catalog # M01571. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AIF AIFM1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01571)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GH-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human AIF
Reactive Species
M01571 is reactive to AIFM1 in Human, Mouse, Rat
Observed Molecular Weight
70 kDa
Calculated molecular weight
66.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01571 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:50
Positive Control
WB: human K562 whole cell, human Hela whole cell, human Jurkat whole cell, human 293T whole cell, rat C6 whole cell, rat RH35 whole cell, mouse Neuro-2a whole cell, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of AIF using anti-AIF antibody (M01571).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse Neuro-2a whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AIF antigen affinity purified monoclonal antibody (M01571) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AIF at approximately 70 kDa. The expected band size for AIF is at 67 kDa.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Click image to see more details
Immunofluorescent analysis of Hela cells, using AIF Antibody .
Specific Publications For Anti-AIF AIFM1 Rabbit Monoclonal Antibody (M01571)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-AIF AIFM1 Rabbit Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-AIF AIFM1 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-AIF AIFM1 Rabbit Monoclonal Antibody
Question
We have seen staining in human brain. Are there any suggestions? Is anti-AIF Rabbit Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
From what I have seen in literature brain does express AIFM1. From what I have seen in Uniprot.org, AIFM1 is expressed in apex of heart, kidney, brain, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have AIFM1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10913597, 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Kidney, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-11-25
Question
My colleagues were satisfied with the WB result of your anti-AIF Rabbit Monoclonal antibody. However we have seen positive staining in apex of heart mitochondrion intermembrane space. using this antibody. Is that expected? Could you tell me where is AIFM1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-08-01
Answer
Based on literature, apex of heart does express AIFM1. Generally AIFM1 expresses in mitochondrion intermembrane space., isoform 3: mitochondrion intermembrane space, isoform 5: cytoplasm. Regarding which tissues have AIFM1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10913597, 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Kidney, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2018-08-01
Question
We are currently using anti-AIF Rabbit Monoclonal antibody M01571 for rat tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
S. Mangal
Verified customer
Asked: 2018-07-10
Answer
The anti-AIF Rabbit Monoclonal antibody (M01571) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-10
Question
We ordered your anti-AIF Rabbit Monoclonal antibody for IP on brain in a previous project. I am using mouse, and We intend to use the antibody for IF next. I was wanting to use examining brain as well as apex of heart in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2018-06-18
Answer
I have checked the website and datasheets of our anti-AIF Rabbit Monoclonal antibody and it appears that M01571 has been tested on mouse in both IP and IF. Thus M01571 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-06-18
Question
We want using your anti-AIF Rabbit Monoclonal antibody for cellular response to nitric oxide studies. Has this antibody been tested with western blotting on k562 cell lysate? We would like to see some validation images before ordering.
L. Collins
Verified customer
Asked: 2014-07-16
Answer
Thanks for your inquiry. This M01571 anti-AIF Rabbit Monoclonal antibody is tested on k562 cell lysate. It is guaranteed to work for IP, IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-07-16
