Anti-AIF AIFM1 Rabbit Monoclonal Antibody

AIF antibody

Boster Bio Anti-AIF AIFM1 Rabbit Monoclonal Antibody catalog # M01571. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01571
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

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Product Name

Anti-AIF AIFM1 Rabbit Monoclonal Antibody

View all AIF Antibodies

SKU/Catalog Number

M01571

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-AIF AIFM1 Rabbit Monoclonal Antibody catalog # M01571. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AIF AIFM1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01571)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

GH-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human AIF

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01571 is reactive to AIFM1 in Human, Mouse, Rat

Applications

M01571 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

66.901kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:500
ICC/IF 1:100-1:500
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For AIFM1 (Source: Uniprot.org, NCBI)

Gene Name

AIFM1

Full Name

Apoptosis-inducing factor 1, mitochondrial

Weight

66.901kDa

Superfamily

FAD-dependent oxidoreductase family

Alternative Names

AIF; AIFapoptosis-inducing factor 1, mitochondrial; AIFM1; apoptosis-inducing factor, mitochondrion-associated, 1; COXPD6; MGC111425; PDCD8; PDCD8striatal apoptosis-inducing factor; programmed cell death 8 (apoptosis-inducing factor); Programmed cell death protein 8 AIFM1 AIF, AUNX1, CMT2D, CMTX4, COWCK, COXPD6, DFNX5, NADMR, NAMSD, PDCD8, SEMDHL apoptosis inducing factor mitochondria associated 1 apoptosis-inducing factor 1, mitochondrial|apoptosis-inducing factor, mitochondrion-associated, 1|auditory neuropathy, X-linked recessive 1|programmed cell death 8 (apoptosis-inducing factor)|striatal apoptosis-inducing factor|testicular secretory protein Li 4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on AIFM1, check out the AIFM1 Infographic

AIFM1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for AIFM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-AIF AIFM1 Rabbit Monoclonal Antibody

Question

We have seen staining in human brain. Are there any suggestions? Is anti-AIF Rabbit Monoclonal antibody supposed to stain brain positively?

Verified Customer

Verified customer

Asked: 2019-11-25

Answer

From what I have seen in literature brain does express AIFM1. From what I have seen in Uniprot.org, AIFM1 is expressed in apex of heart, kidney, brain, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have AIFM1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10913597, 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Kidney, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2019-11-25

Question

My colleagues were satisfied with the WB result of your anti-AIF Rabbit Monoclonal antibody. However we have seen positive staining in apex of heart mitochondrion intermembrane space. using this antibody. Is that expected? Could you tell me where is AIFM1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-08-01

Answer

Based on literature, apex of heart does express AIFM1. Generally AIFM1 expresses in mitochondrion intermembrane space., isoform 3: mitochondrion intermembrane space, isoform 5: cytoplasm. Regarding which tissues have AIFM1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10913597, 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Kidney, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2018-08-01

Question

We are currently using anti-AIF Rabbit Monoclonal antibody M01571 for rat tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?

S. Mangal

Verified customer

Asked: 2018-07-10

Answer

The anti-AIF Rabbit Monoclonal antibody (M01571) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-07-10

Question

We ordered your anti-AIF Rabbit Monoclonal antibody for IP on brain in a previous project. I am using mouse, and We intend to use the antibody for IF next. I was wanting to use examining brain as well as apex of heart in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?

Verified Customer

Verified customer

Asked: 2018-06-18

Answer

I have checked the website and datasheets of our anti-AIF Rabbit Monoclonal antibody and it appears that M01571 has been tested on mouse in both IP and IF. Thus M01571 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-06-18

Question

We want using your anti-AIF Rabbit Monoclonal antibody for cellular response to nitric oxide studies. Has this antibody been tested with western blotting on k562 cell lysate? We would like to see some validation images before ordering.

L. Collins

Verified customer

Asked: 2014-07-16

Answer

Thanks for your inquiry. This M01571 anti-AIF Rabbit Monoclonal antibody is tested on k562 cell lysate. It is guaranteed to work for IP, IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2014-07-16

Size

Total: $370

SKU:M01571

Backordered.

Lead time for this item is typically 3-4 weeks

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M01571
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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