Anti-ACSL3 Antibody Picoband™

ACSL3 antibody

Boster Bio Anti-ACSL3 Antibody Picoband™ catalog # A05945. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A05945
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-ACSL3 Antibody Picoband™

View all ACSL3 Antibodies

SKU/Catalog Number

A05945

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ACSL3 Antibody Picoband™ catalog # A05945. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ACSL3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05945)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ACSL3 recombinant protein (Position: M12-I224). Human ACSL3 shares 91.1% and 90.1% amino acid (aa) sequence identity with mouse and rat ACSL3, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05945 is reactive to ACSL3 in Human

Applications

A05945 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

80 kDa

Calculated molecular weight

80.42kDa

Background of ACSL3

Long-chain-fatty-acid—CoA ligase 3 is an enzyme that in humans is encoded by the ACSL3 gene. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For ACSL3 (Source: Uniprot.org, NCBI)

Gene Name

ACSL3

Full Name

Long-chain-fatty-acid--CoA ligase 3

Weight

80.42kDa

Superfamily

ATP-dependent AMP-binding enzyme family

Alternative Names

ACS3EC 6.2.1.3; acyl-CoA synthetase long-chain family member 3; FACL3lignoceroyl-CoA synthase; fatty-acid-Coenzyme A ligase, long-chain 3; LACS 3; LACS3; Long-chain acyl-CoA synthetase 3; long-chain-fatty-acid--CoA ligase 3; PRO2194 ACSL3 ACS3, FACL3, LACS 3, LACS3, PRO2194 acyl-CoA synthetase long chain family member 3 long-chain-fatty-acid--CoA ligase 3|arachidonate--CoA ligase|fatty-acid-Coenzyme A ligase, long-chain 3|lignoceroyl-CoA synthase|long-chain acyl-CoA synthetase 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ACSL3, check out the ACSL3 Infographic

ACSL3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ACSL3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-ACSL3 Antibody Picoband™

Question

I was wanting to use to test anti-ACSL3 antibody A05945 on human placenta for research purposes, then I may be interested in using anti-ACSL3 antibody A05945 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

P. Anderson

Verified customer

Asked: 2018-10-11

Answer

The products we sell, including anti-ACSL3 antibody A05945, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-10-11

Question

I see that the anti-ACSL3 antibody A05945 works with WB, what is the protocol used to produce the result images on the product page?

W. Roberts

Verified customer

Asked: 2017-11-23

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-11-23

Question

We are currently using anti-ACSL3 antibody A05945 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?

H. Banerjee

Verified customer

Asked: 2017-09-12

Answer

The anti-ACSL3 antibody (A05945) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-09-12

Question

My question regarding product A05945, anti-ACSL3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

E. Parker

Verified customer

Asked: 2014-07-21

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05945 anti-ACSL3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-07-21

Size

Conjugation

Total: $370

SKU:A05945

Backordered.

Lead time for this item is typically 2-3 weeks

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A05945
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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