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Product Info Summary
| SKU: | A05945 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ACSL3 Antibody Picoband®
SKU/Catalog Number
A05945
Size
100 μg/vial
Form
Lyophilized
Description
Pathways/functions (putative): long-chain acyl-CoA synthetase 3 activates fatty acids to acyl-CoAs → β-oxidation and lipid synthesis; implicated in cellular lipid rewiring. Assay: rabbit polyclonal for WB (human). Often assessed with enterocyte lipid marker FABP2/I-FABP to link uptake/binding with activation; can be juxtaposed with immune mediator IL-12A for immunometabolic context, and with BDNF in studies of metabolism–neuronal signaling crosstalk (putative).
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ACSL3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05945)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ACSL3 recombinant protein (Position: M12-I224). Human ACSL3 shares 91.1% and 90.1% amino acid (aa) sequence identity with mouse and rat ACSL3, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05945 is reactive to ACSL3 in Human
Observed Molecular Weight
80 kDa
Calculated molecular weight
80.4 kDa
Background of ACSL3
Long-chain-fatty-acid—CoA ligase 3 is an enzyme that in humans is encoded by the ACSL3 gene. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05945 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HEPG2 whole cell
Validation Images & Assay Conditions
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Western blot analysis of ACSL3 using anti-ACSL3 antibody (A05945).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: HEPG2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACSL3 antigen affinity purified polyclonal antibody (Catalog # A05945) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACSL3 at approximately 80KD. The expected band size for ACSL3 is at 80KD.
Specific Publications For Anti-ACSL3 Antibody Picoband® (A05945)
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4 Customer Q&As for Anti-ACSL3 Antibody Picoband®
Question
I was wanting to use to test anti-ACSL3 antibody A05945 on human placenta for research purposes, then I may be interested in using anti-ACSL3 antibody A05945 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Anderson
Verified customer
Asked: 2018-10-11
Answer
The products we sell, including anti-ACSL3 antibody A05945, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-10-11
Question
I see that the anti-ACSL3 antibody A05945 works with WB, what is the protocol used to produce the result images on the product page?
W. Roberts
Verified customer
Asked: 2017-11-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-11-23
Question
We are currently using anti-ACSL3 antibody A05945 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
H. Banerjee
Verified customer
Asked: 2017-09-12
Answer
The anti-ACSL3 antibody (A05945) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-12
Question
My question regarding product A05945, anti-ACSL3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Parker
Verified customer
Asked: 2014-07-21
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05945 anti-ACSL3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-07-21
