Anti-IL-12A Antibody

IL12A antibody

Boster Bio Anti-IL-12A Antibody catalog # A00918. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: A00918
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC-P, WB

Product Name

Anti-IL-12A Antibody

View all IL12A Antibodies

SKU/Catalog Number

A00918

Size

100μl

Form

Liquid

Description

Boster Bio Anti-IL-12A Antibody catalog # A00918. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IL-12A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00918)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

5D7

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human IL-12A.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00918 is reactive to IL12A in Human, Mouse, Rat

Applications

A00918 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

24.874kDa

Background of IL12A

Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions

Lamalice L, et al. (2004). Oncogene.23(2): 434-445.
Takahashi T, et al. (2001). EMBO J .20(11): 2768-2778.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC-p 1:50-300
ELISA 1:2000

Validation Images & Assay Conditions

Gene/Protein Information For IL12A (Source: Uniprot.org, NCBI)

Gene Name

IL12A

Full Name

Interleukin-12 subunit alpha

Weight

24.874kDa

Superfamily

IL-6 superfamily

Alternative Names

CLMF p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL12 p70; IL-12 p70; IL-12 subunit p35; IL-12, subunit p35; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35 IL12A CLMF, IL-12A, NFSK, NKSF1, P35 interleukin 12A interleukin-12 subunit alpha|CLMF p35|IL-12, subunit p35|IL35 subunit|NF cell stimulatory factor chain 1|NK cell stimulatory factor chain 1|cytotoxic lymphocyte maturation factor 1, p35|cytotoxic lymphocyte maturation factor 35 kDa subunit|interleukin 12, p35|interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)|interleukin-12 alpha chain

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on IL12A, check out the IL12A Infographic

IL12A infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IL12A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00918 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Jiang Peng-Fei,Peng Jun,Tan Han-Yu,Ou-Yang Yun,Qin Gen-Yan,Peng Qing-Hua,Effects of Buddlejae Flos Granules on Inflammatory Factors TGF-β1, NF-κB, IL-10 and IL-12 in Lacrimal Gland Cells of Castrated Male Rabbits,Digital Chinese Medicine,Volume 2,Issue 2,2019,Pages 97-104,ISSN 2589-3777,https://doi.org/10.1016/j.dcmed.2019.09.004.
Species: New Zealand White Rabbit
A00918 usage in article: APP:IHC, SAMPLE:LACRIMAL GLAND CELL, DILUTION:NA

Re-polarizing Myeloid-derived Suppressor Cells (MDSCs) with Cationic Polymers for Cancer Immunotherapy

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7 Customer Q&As for Anti-IL-12A Antibody

Question

Is there a BSA free version of anti-IL-12A antibody A00918 available?

W. Kulkarni

Verified customer

Asked: 2019-12-19

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL-12A antibody A00918 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-19

Question

I see that the anti-IL-12A antibody A00918 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-10-30

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-10-30

Question

I was wanting to use your anti-IL-12A antibody for WB for rat lower esophagus mucosa on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat lower esophagus mucosa identification?

Verified Customer

Verified customer

Asked: 2019-07-09

Answer

You can see on the product datasheet, A00918 anti-IL-12A antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat lower esophagus mucosa in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-09

Question

Will anti-IL-12A antibody A00918 work on primate WB with lower esophagus mucosa?

Verified Customer

Verified customer

Asked: 2019-01-29

Answer

Our lab technicians have not tested anti-IL-12A antibody A00918 on primate. You can run a BLAST between primate and the immunogen sequence of anti-IL-12A antibody A00918 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate lower esophagus mucosa in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-01-29

Question

Is a blocking peptide available for product anti-IL-12A antibody (A00918)?

Verified Customer

Verified customer

Asked: 2018-03-12

Answer

We do provide the blocking peptide for product anti-IL-12A antibody (A00918). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-03-12

Question

Is this A00918 anti-IL-12A antibody reactive to the isotypes of IL12A?

G. Patel

Verified customer

Asked: 2018-01-02

Answer

The immunogen of A00918 anti-IL-12A antibody is Synthesized peptide derived from the Internal region of human IL-12A. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-01-02

Question

My question regards to test anti-IL-12A antibody A00918 on rat lower esophagus mucosa for research purposes, then I may be interested in using anti-IL-12A antibody A00918 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

S. Collins

Verified customer

Asked: 2014-11-04

Answer

The products we sell, including anti-IL-12A antibody A00918, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2014-11-04

Order DetailsPrice
A00918

100uL

$399

Free Secondary Antibody

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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