Skip to the beginning of the images gallery

Bdnf antibody (PB9075) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 49 Publications

pb9075 bdnf primary antibodies ihc testing 4

IHC analysis of BDNF using anti-BDNF antibody (PB9075).
BDNF was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BDNF Antibody (PB9075) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Bundle Save

Bundle & Save Program

Save up to 50% on Picoband® antibodies + earn 10% back on eligible partner reagents.

View Details

BUY MORE, SAVE MORE

This antibody qualifies!
2 = 20% off · 3 = 30% off · 4 = 40% off · 5+ = 50% off

View Details

Free Secondary Antibody

How to redeem? While adding to cart, choose a size with a FREE Secondary Antibody!

View Details

Anti-BDNF Antibody Picoband®

Name: Anti-BDNF Antibody Picoband®
Brand: Boster Bio
SKU: PB9075
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

Bdnf antibody

49 Publications|

1 Review

|Submit a Review

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	IHC, WB

Tested Applications

Bdnf antibody was positively detected by WB in:

recombinant human BDNF protein

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

Bdnf antibody was positively detected by IHC in:

mouse brain tissue, rat brain tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Mouse

Tested

Recommend

Rat

Tested

Recommend

Product Information

Product Name

Anti-BDNF Antibody Picoband®
View all BDNF Antibodies

Form

Lyophilized

Uniprot

P21237

Gene ID

12064

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

Anti-BDNF Picoband™ Antibody (PB9075) was raised against E.coli-derived human BDNF recombinant protein (Position: H129-R247). Human BDNF shares 100% amino acid (aa) sequence identity with both mouse and rat BDNF.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

14 kDa

Calculated Molecular Weight

28.1 kDa

Background of Bdnf

Brain-derived neurotrophic factor, also known as BDNF, is a secreted protein that, in humans, is encoded by the BDNF gene. BDNF is a member of the neurotrophin family of growth factors, which are related to the canonical nerve growth factor. It is mapped to 11p14.1. BDNF is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is expressed within peripheral ganglia and is not restricted to neuronal target fields. BDNF has been purified and shown to reduce the amount of naturally occurring neuronal cell death in portions of the peripheral nervous system.

Cite This Product

Anti-BDNF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9075)

Protocols

Western Blot protocol for Bdnf antibody

Review Protocol

Immunohistochemistry (IHC) protocol for Bdnf antibody

Review Protocol

Publications

GLGZG improves post-stroke spastic paralysis in rats through regulating BDNF/TrkB signaling

Published: 2026-08-25

Journal: 3 Biotech

Application: IHC,WB

Impact Factor: 3.10

Species: Rat

PMID: 42643758

SKU	PB9075
Application	Western Blot
Sample	mouse brain tissue
Sample Processing Description	① Hippocampal tissue from normal mice, ② Hippocampal tissue from depressed mice, ③ Hippocampal tissue from depressed mice treated with our in-house drug, ④ Hippocampal tissue from depressed mice treated with a positive control drug. Total protein was extracted from all samples.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody	BDNF Antibody Picoband®
Primary Incubation	1:2000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation	1:10000, 1 h in RT
Detection	Substrate: ECL substrate, Image system: ChemiDoc MP
Results Summary	BDNF belongs to the neurotrophin family and functions by binding to the TrkB receptor on the surface of neurons, activating intracellular signaling pathways to maintain, protect, and enhance neuronal function. In simple terms, it helps brain cells stay healthier, more active, and better connected. Experimental results show that BDNF levels are significantly decreased in the brains of depressed mice, but are restored after treatment.

Bundle Save

Bundle & Save Program

BUY MORE, SAVE MORE

Free Secondary Antibody

Anti-BDNF Antibody Picoband®

Bdnf antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

GLGZG improves post-stroke spastic paralysis in rats through regulating BDNF/TrkB signaling

Free fatty acid 1 (FFA1) receptor modulation in experimental arthritis in male and female mice subjected to environmental enrichment

L-tetrahydropalmatine attenuates ketamine reward effect via modulating the miR-27a-3p/MAP2K4 axis

Pharmacological Inhibition of Tropomyosin Receptor Kinase B Reverses Remifentanil-Induced Postoperative Hyperalgesia and Synaptic Remodeling

Resveratrol Attenuates Neuroinflammation and Gut–Brain Axis Dysfunction Induced by Lead and Cadmium Co-Exposure by Modulating Gut Microbiota in Rats

Single administration of vitamin C produces rapid antidepressant-like effects in female mice: A possible role of dopamine D2 receptor signalling

Construction of ROS-responsive cascade-targeted liposomes and investigation of their anti-AD effects

Multi-omics analysis reveals the mechanism of action of Atractylodin against depression

Chrysin modulates the BDNF/TrkB/AKT/Creb neuroplasticity signaling pathway: Acting in the improvement of cognitive flexibility and declarative, working and aversive memory deficits caused by hypothyroidism in C57BL/6 female mice

Neonatal Maternal Separation Impairs Cognitive Function and Synaptic Plasticity in Adult Male CD-1 Mice

Human induced pluripotent stem cell/embryonic stem cell-derived pyramidal neuronal precursors show safety and efficacy in a rat spinal cord injury model

Menthol-Modified Quercetin Liposomes with Brain-Targeting Function for the Treatment of Senescent Alzheimer’s Disease

The beneficial effects of vortioxetine on BDNF, CREB, S100B, β amyloid, and glutamate NR2b receptors in chronic unpredictable mild stress model of depression

Biological effects of exposure to 2650 MHz electromagnetic radiation on the behavior, learning, and memory of mice

Plasminogen decreases Aβ42 and Tau deposition, and shows multi-beneficial effects on Alzheimer's disease in mice and humans

Soluble dietary fiber and cellulose from Saccharina japonica by-product ameliorate Loperamide-induced constipation via modulating enteric neurotransmitters, short-chain fatty acids and gut microbiota

SIRT1 modifies DNA methylation linked to synaptic deficits induced by Pb in vitro and in vivo

Study on the mechanism of visual aging in cats’ primary visual cortex based on BDNF-TrkB signal pathway

SPIONs mediated magnetic actuation promotes nerve regeneration by inducing and maintaining repair-supportive phenotypes in Schwann cells

Central and peripheral effects of environmental enrichment in a mouse model of arthritis

Agomelatine Softens Depressive-Like Behavior through the Regulation of Autophagy and Apoptosis

Berberine Suppresses Mice Depression Behaviors and Promotes Hippocampal Neurons Growth Through Regulating the miR-34b-5p/miR-470-5p/BDNF Axis

Effect of C-phycocyanin on HDAC3 and miRNA-335 in Alzheimer’s disease

Influence of hippocampal low-frequency stimulation on GABAA R α1, ICER and BNDF expression level in brain tissues of amygdala-kindled drug-resistant temporal lobe epileptic rats

Abnormal expressions of AGEs, TGF-β1, BDNF and their receptors in diabetic rat colon–Associations with colonic morphometric and biomechanical remodeling

Resveratrol prevents cognitive deficits induced by chronic unpredictable mild stress: Sirt1/miR-134 signalling pathway regulates CREB/BDNF expression in hippocampus in vivo and in vitro

Intravenously Delivered Allogeneic Mesenchymal Stem Cells Bidirectionally Regulate Inflammation and Induce Neurotrophic Effects in Distal Middle Cerebral Artery Occlusion Rats Within the First 7 Days After Stroke

Perineurium-like sheath derived from long-term surviving mesenchymal stem cells confers nerve protection to the injured spinal cord

Effect of hippocampal L‑NBP on BDNF and TrkB expression and neurological function of vascular dementia rats

The Proliferation Enhancing Effects of Salidroside on Schwann Cells In Vitro

Therapeutic efficacy of neural stem cells originating from umbilical cord-derived mesenchymal stem cells in diabetic retinopathy.

Synergistic effects of G-CSF and bone marrow stromal cells on nerve regeneration with acellular nerve xenografts

Electroacupuncture Ameliorates Learning and Memory and Improves Synaptic Plasticity via Activation of the PKA/CREB Signaling Pathway in Cerebral Hypoperfusion

Dexmedetomidine protects against learning and memory impairments caused by electroconvulsive shock in depressed rats: Involvement of the NMDA receptor subunit 2B (NR2B)-ERK signaling pathway

Ginkgo biloba and vitamin E ameliorate haloperidol-induced vacuous chewingmovement and brain-derived neurotrophic factor expression in a rat tardive dyskinesia model

BDNF-induced expansion of cumulus-oocyte complexes in pigs was mediated by microRNA-205

Bone marrow-derived mesenchymal stem cells differentiate into nerve-like cells in vitro after transfection with brain-derived neurotrophic factor gene

Protocatechuic acid improves cognitive deficits and attenuates amyloid deposits, inflammatory response in aged AβPP/PS1 double transgenic mice

Enhanced neural differentiation of neural stem cells and neurite growth by amniotic epithelial cell co-culture

Changes in compressed neurons from dogs with acute and severe cauda equina constrictions following intrathecal injection of brain-derived neurotrophic factor-conjugated polymer nanoparticles

Cellular and molecular mechanisms underlying the action of ginsenoside Rg1 against Alzheimer's disease

Sciatic nerve repair by acellular nerve xenografts implanted with BMSCs in rats xenograft combined with BMSCs

The expression and putative role of brain-derived neurotrophic factor and its receptor in bovine sperm

Neural Stem Cells Over-Expressing Brain-Derived Neurotrophic Factor (BDNF) Stimulate Synaptic Protein Expression and Promote Functional Recovery Following Transplantation in Rat Model of Traumatic Brain Injury

Acute Stress and Chronic Stress Change Brain-Derived Neurotrophic Factor (BDNF) and Tyrosine Kinase-Coupled Receptor (TrkB) Expression in Both Young and Aged Rat Hippocampus

Icariin isolated from Epimedium brevicornum Maxim attenuates learning and memory deficits induced by d-galactose in rats

Protective Effect of Total Flavones of Abelmoschus manihot L. Medic Against Poststroke Depression Injury in Mice and Its Action Mechanism

Catalpol increases hippocampal neuroplasticity and up-regulates PKC and BDNF in the aged rats

Moclobemide up-regulates proliferation of hippocampal progenitor cells in chronically stressed mice

Review

Have you used Anti-BDNF Antibody Picoband®?

1 Review For Anti-BDNF Antibody Picoband®

Anti-BDNF (PB9075) shows clear, specific bands in mouse brain tissues by WB, with BDNF levels markedly reduced in depressed mice and restored after treatment, demonstrating excellent antibody performance.

Product Promise

Troubleshooting

Boster Guarantee

Guaranteed product quality