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Product Info Summary
| SKU: | H01263 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-beta Actin Mouse Monoclonal Antibody(HRP Conjugated)
SKU/Catalog Number
H01263
BM5180 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-beta Actin Mouse Monoclonal Antibody(HRP Conjugated) catalog # H01263. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-beta Actin Mouse Monoclonal Antibody(HRP Conjugated) (Boster Biological Technology, Pleasanton CA, USA, Catalog # H01263)
Host
Mouse
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
17A12
Isotype
IgG2b
Immunogen
A synthesized peptide derived from human beta Actin
Reactive Species
H01263 is reactive to ACTB in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
41.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
H01263 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Validation Images & Assay Conditions
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Western blot analysis of beta Actin expression in (1) Hela cell lysate, (2) Human liver lysate, (3) 3T3 cell lysate, (4) Mouse brain lysate, (5) PC12 cell lysate, (6) Rat heart lysate.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Specific Publications For Anti-beta Actin Mouse Monoclonal Antibody(HRP Conjugated) (H01263)
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