Human MMP-2 ELISA Kit PicoKine™

Human MMP-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MMP2 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. Cited in 27 publication(s).

Product Info Summary

SKU: EK0459
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin).

Product Name

Human MMP-2 ELISA Kit PicoKine™

See all MMP-2 products

SKU/Catalog Number

EK0459

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human MMP-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MMP2 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human MMP-2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0459)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A30-C660

Sensitivity

<10 pg/ml

Assay Range

625 pg/ml - 40,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0459 is reactive to MMP2 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin).

Application Guarantee

EK0459 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0459-CAP Anti-Human MMP2 Pre-coated 96-well strip microplate 1
EK0459-ST Human MMP2 Standard 2 vials, 40 ng/tube
EK0459-DA Human MMP2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)0625125025005000100002000040000
O.D.0.0330.0550.1120.1920.3130.5381.0411.812

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect MMP-2, Dilution ratio of 1:10, concentration in serum is around 50ng/ml.

Some articles we found to cite concentrations of MMP-2 in samples: 17239302 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human MMP-2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)13263925140991253397014093
Standard deviation80.88282.6690.8576.43349.36690.55
CV (%)6.1%7.2%4.9%6.1%8.8%4.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of MMP-2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11326136513681233132354.534.1%
Sample 239253610342638833711204.25.5%
Sample 314099163301408316849153401262.668.2%
*number of samples for each test n=16.

Gene/Protein Information For MMP2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P08253

Gene ID

4313

Gene Name

MMP2

Full Name

72 kDa type IV collagenase

Weight

73.882kDa

Superfamily

peptidase M10A family

Alternative Names

72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase) MMP2 CLG4, CLG4A, MMP-2, MMP-II, MONA, TBE-1 matrix metallopeptidase 2 72 kDa type IV collagenase|collagenase type IV-A|matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)|matrix metalloproteinase-2|matrix metalloproteinase-II|neutrophil gelatinase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MMP2, check out the MMP2 Infographic

MMP2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MMP2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0459 has been cited in 27 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wang H,Yuan M,Yang E,Chen D,Su A,Wu Z.Enterovirus 71 infection induced Aquaporin-4 depolarization by increasing matrix metalloproteinase-9 activity.Neurosci Lett.2021 Jun 11:136049.doi:10.1016/j.neulet.2021.136049.Epub ahead of print.PMID:34126180.
Species: Human,Mouse
EK0459 usage in article: APP:ELISA, SAMPLE:CSF, DILUTION:NA

Cosentino G,Di Stefano V,Presti RL,Montana M,Todisco M,Gastaldi M,Cortese A,Alfonsi E,Tassorelli C,Fierro B,Caimi G,Brighina F.Expression pattern of matrix metalloproteinases-2 and -9 and their tissue inhibitors in patients with chronic inflammatory demyelinating polyneuropathy.Neurol Sci.2021 May 14.doi:10.1007/s10072-021-05314-y.Epub ahead of print.PMID:33988800.
Species: Human
EK0459 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Hu S, Zheng W, Jin L. Astragaloside IV inhibits cell proliferation and metastasis of breast cancer via promoting the long noncoding RNA TRHDE-AS1. J Nat Med. 2020 Nov 20. doi: 10.1007/s11418-020-01469-8. Epub ahead of print. PMID: 33219447.
Species: Human,Mouse
EK0459 usage in article: APP:ELISA, SAMPLE:MCF-10A CELLS,MCF-7 CELLS,MDA-MB-231 CELLS AND MDA-MB-468 CELLS, DILUTION:NA

Behaviour of the plasma concentration of gelatinases and their tissue inhibitors in subjects with venous leg ulcers

CXCL10/CXCR3 axis promotes the invasion of gastric cancer via PI3K/AKT pathway-dependent MMPs production

Expression levels and association of gelatinases MMP-2 and MMP-9 and collagenases MMP-1 and MMP-13 with VEGF in synovial fluid of patients with arthritis

Resveratrol Suppresses Matrix Metalloproteinase-2 Activation Induced by Lipopolysaccharide in Mouse Osteoblasts via Interactions with AMP-Activated Protein %u2026

Salivary MMP-1, MMP-2, MMP-3 and MMP-13 Levels in Patients with Oral Lichen Planus and Squamous Cell Carcinoma

KATP channels in high glucose-induced rat mesangial cell proliferation and release of MMP-2 and fibronectin

miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBP?

The Angiogenic Effect of microRNA-21 Targeting TIMP3 through the Regulation of MMP2 and MMP9

Role of Sphingosine Kinase/S1P Axis in ECM Remodeling of Cardiac Cells Elicited by Relaxin

Overexpression of HTRA1 Leads to Down-Regulation of Fibronectin and Functional Changes in RF/6A Cells and HUVECs

Matin S, Nemati A, Ghobadi H, Alipanah-Moghadam R, Rezagholizadeh L. Int J Chron Obstruct Pulmon Dis. 2018 May 3;13:1449-1454. doi: 10.2147/COPD.S155985. eCollection 2018. The effect of conjugated linoleic acid on oxidative stress and matrix metal...

Qin Y, Liu Xj, Li L, Liu Xj, Li Y, Gao Rj, Shao Rg, Zhen Ys. Oncol Rep. 2014 Jul;32(1):121-30. Doi: 10.3892/Or.2014.3169. Epub 2014 May 7. Mmp-2/9-Oriented Combinations Enhance Antitumor Efficacy Of Egfr/Her2-Targeting Fusion Proteins And Gemcitab...

Simmers P, Gishto A, Vyavahare N, Kothapalli Cr. Tissue Eng Part A. 2015 Apr;21(7-8):1455-70. Doi: 10.1089/Ten.Tea.2014.0363. Epub 2015 Mar 9. Nitric Oxide Stimulates Matrix Synthesis And Deposition By Adult Human Aortic Smooth Muscle Cells Within...

Xu Xl, Ling Dy, Zhu Qy, Fan Wj, Zhang W. Eur J Pharmacol. 2013 Jan 5;698(1-3):370-8. Doi: 10.1016/J.Ejphar.2012.11.019. Epub 2012 Nov 21. The Effect Of 2,3,4',5-Tetrahydroxystilbene-2-0-??-D Glucoside On Neointima Formation In A Rat Artery Balloon...

Tang Xp, Tang Gd, Fang Cy, Liang Zh, Zhang Ly. World J Gastroenterol. 2013 Mar 14;19(10):1582-92. Doi: 10.3748/Wjg.V19.I10.1582. Effects Of Ginsenoside Rh2 On Growth And Migration Of Pancreatic Cancer Cells.

Yuan H, Yang P, Zhou D, Gao W, Qiu Z, Fang F, Ding S, Xiao W. Mol Biol Rep. 2014 Aug;41(8):5157-65. Doi: 10.1007/S11033-014-3382-4. Epub 2014 May 10. Knockdown Of Sphingosine Kinase 1 Inhibits The Migration And Invasion Of Human Rheumatoid Arthrit...

Mazani M, Fard As, Baghi An, Nemati A, Mogadam Ra. J Pak Med Assoc. 2014 Jul;64(7):785-90. Effect Of Pomegranate Juice Supplementation On Matrix Metalloproteinases 2 And 9 Following Exhaustive Exercise In Young Healthy Males.

Liu Q,, Zou R, Zhou R, Gong C, Wang Z, Cai T, Tan C, Fang J. J Cell Biochem. 2015 Jul;116(7):1213-21. Doi: 10.1002/Jcb.25073. Mir-155 Regulates Glioma Cells Invasion And Chemosensitivity By P38 Isforms In Vitro.

Pan B, Ren H, Ma Y, Liu D, Yu B, Ji L, Pan L, Li J, Yang L, Lv X, Shen X, Chen B, Zhang Y, Willard B, He Y, Zheng L. Int J Cancer. 2012 Jul 1;131(1):70-82. Doi: 10.1002/Ijc.26341. Epub 2011 Aug 30. High-Density Lipoprotein Of Patients With Type 2 ...

Yu X, Zhu J, Mi M, Chen W, Pan Q, Wei M. Med Oncol. 2012 Mar;29(1):349-57. Doi: 10.1007/S12032-010-9770-2. Epub 2010 Dec 4. Anti-Angiogenic Genistein Inhibits Vegf-Induced Endothelial Cell Activation By Decreasing Ptk Activity And Mapk Activation.

Hopps E, Lo Presti R, Montana M, Noto D, Averna Mr, Caimi G. J Investig Med. 2013 Aug;61(6):978-83. Doi: 10.231/Jim.0B013E318294E9Da. Gelatinases And Their Tissue Inhibitors In A Group Of Subjects With Metabolic Syndrome.

Hopps E, Lo Presti R, Montana M, Canino B, Averna Mr, Caimi G. Mediators Inflamm. 2014;2014:510619. Doi: 10.1155/2014/510619. Epub 2014 Jul 10. Study Of The Correlations Among Some Parameters Of The Oxidative Status, Gelatinases, And Their Inhibit...

Gishto A, Farrell K, Kothapalli Cr. J Biomed Mater Res A. 2015 Feb;103(2):693-708. Doi: 10.1002/Jbm.A.35217. Epub 2014 May 19. Tuning Composition And Architecture Of Biomimetic Scaffolds For Enhanced Matrix Synthesis By Murine Cardiomyocytes.

Kim Ks, Lee Ya, Choi Hm, Yoo Mc, Yang Hi. Rheumatol Int. 2012 Oct;32(10):3069-75. Epub 2011 Sep 13. Implication Of Mmp-9 And Urokinase Plasminogen Activator (Upa) In The Activation Of Pro-Matrix Metalloproteinase (Mmp)-13.

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8 Customer Q&As for Human MMP-2 ELISA Kit PicoKine™

Question

Would EK0459 be suitable to measure pro-MMP2 in human Serum and EDTA-plasma samples?

Verified customer

Asked: 2021-01-25

Answer

EDTA-plasma is not recommended to be used in this assay due to its chelating property. The Human MMP-2 ELISA Kit PicoKine™ (EK0459) detects total MMP2 including pro and mature MMP-2 and it is not specific to detect pro-MMP2 only.

Boster Scientific Support

Answered: 2021-01-26

Question

Do these ELISA kits (Human MMP-2 & Human MMP-3) use the same sample diluent? What kind of chemicals are present in this diluent?

Verified Customer

Verified customer

Asked: 2019-09-11

Answer

Yes. The diluent contains NaCl, Tris, BSA and preservative.

Boster Scientific Support

Answered: 2019-09-11

Question

Q: how to analyze ELISA data? I measured MMP-2 level in plasma.

A. Kapoor

Verified customer

Asked: 2019-02-12

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-02-12

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-04-06

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-04-06

Question

Q: Can MMP-2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2016-08-13

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2016-08-13

Question

Q: What is the optimal O.D. value for MMP-2 ELISA kit? I performed your MMP-2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain MMP-2 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2015-09-02

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this MMP-2 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of MMP-2 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2015-09-02

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring MMP-2 using the EK0459 Human MMP-2 Picokine® ELISA Kit?

H. Martin

Verified customer

Asked: 2015-08-23

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2015-08-23

Question

Does EK0459 detect total MMP-2 (both pro & active forms)?

Verified Customer

Verified customer

Asked: 2015-07-23

Answer

EK0459 is able to detect total MMP-2 (pro &

Boster Scientific Support

Answered: 2015-07-23

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