Anti-BMAL1/ARNTL Antibody Picoband™

Product Name

Anti-BMAL1/ARNTL Antibody Picoband™

See all BMAL1 products

SKU/Catalog Number

A00260-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-BMAL1/ARNTL Antibody Picoband™ catalog # A00260-1. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BMAL1/ARNTL Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00260-1)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human BMAL1/ARNTL recombinant protein (Position: H58-D156). Human BMAL1/ARNTL shares 99% and 98% amino acid (aa) sequence identity with mouse and rat BMAL1/ARNTL, respectively.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00260-1 is reactive to ARNTL in Human

Applications

A00260-1 is guaranteed for WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of BMAL1/ARNTL using anti-BMAL1/ARNTL antibody (A00260-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HELA whole Cell lysates,
Lane 2: HEPG2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMAL1/ARNTL antigen affinity purified polyclonal antibody (Catalog # A00260-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMAL1/ARNTL at approximately 69KD. The expected band size for BMAL1/ARNTL is at 69KD.

Gene/Protein Information For ARNTL (Source: Uniprot.Org, NCBI)

Alternative Names

aryl hydrocarbon receptor nuclear translocator-like protein 1; aryl hydrocarbon receptor nuclear translocator-like; basic-helix-loop-helix-PAS orphan MOP3; BHLHE5; bHLHe5brain and muscle; bHLH-PAS protein JAP3; BMAL1TIC; Brain and muscle ARNT-like 1; Class E basic helix-loop-helix protein 5; Member of PAS protein 3; member of PAS superfamily 3; MOP3BMAL1c; PAS domain-containing protein 3; PASD3MGC47515 ARNTL BMAL1, BMAL1c, JAP3, MOP3, PASD3, TIC, bHLHe5 aryl hydrocarbon receptor nuclear translocator like aryl hydrocarbon receptor nuclear translocator-like protein 1|ARNT-like protein 1, brain and muscle|PAS domain containing 3|PAS domain-containing protein 3|bHLH-PAS protein JAP3|basic helix-loop-helix family member e5|basic-helix-loop-helix-PAS orphan MOP3|basic-helix-loop-helix-PAS protein MOP3|brain and muscle ARNT-like 1|class E basic helix-loop-helix protein 5|member of PAS protein 3|member of PAS superfamily 3|testis tissue sperm-binding protein Li 50e

For more info on ARNTL, check out the ARNTL Infographic

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In this infographic you will see the following information for ARNTL: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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