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Product Info Summary
| SKU: | A00260-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-BMAL1/ARNTL Antibody Picoband®
SKU/Catalog Number
A00260-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BMAL1/ARNTL Antibody Picoband® catalog # A00260-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BMAL1/ARNTL Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00260-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human BMAL1/ARNTL recombinant protein (Position: H58-D156). Human BMAL1/ARNTL shares 99% and 98% amino acid (aa) sequence identity with mouse and rat BMAL1/ARNTL, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00260-1 is reactive to ARNTL in Human, Mouse, Rat
Observed Molecular Weight
75 kDa
Calculated molecular weight
68.8 kDa
Background of ARNTL
Aryl hydrocarbon receptor nuclear translocator-like protein 1 is protein that in humans is encoded by the ARNTL gene. The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00260-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human Hela whole cell, human HT1080 whole cell, human MCF-7 whole cell, human SH-SY5Y whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue
FCM: MCF-7 cell
Validation Images & Assay Conditions
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Western blot analysis of BMAL1/ARNTL using anti-BMAL1/ARNTL antibody (A00260-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HT1080 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMAL1/ARNTL antigen affinity purified polyclonal antibody (Catalog # A00260-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMAL1/ARNTL at approximately 75 kDa. The expected band size for BMAL1/ARNTL is at 69 kDa.
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Flow Cytometry analysis of MCF-7 cells using anti-BMAL1/ARNTL antibody (A00260-1).
Overlay histogram showing MCF-7 cells stained with A00260-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BMAL1/ARNTL Antibody (A00260-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-BMAL1/ARNTL Antibody Picoband® (A00260-1)
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Customer Q&As
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5 Customer Q&As for Anti-BMAL1/ARNTL Antibody Picoband®
Question
We have observed staining in human brain. Do you have any suggestions? Is anti-BMAL1/ARNTL antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
According to literature brain does express ARNTL. According to Uniprot.org, ARNTL is expressed in left lobe of thyroid gland, brain, fetal brain, brain skin, among other tissues. Regarding which tissues have ARNTL expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9144434, 14702039
Brain, and Skin, Pubmed ID: 15489334
Fetal brain, Pubmed ID: 9079689
Boster Scientific Support
Answered: 2019-09-19
Question
Has A00260-1 Antibody been tested to react on mouse or rat BMAL1?
Verified customer
Asked: 2019-07-17
Answer
The Anti-BMAL1/ARNTL Antibody Picoband A00260-1 didn't work well on mouse and rat samples.
Boster Scientific Support
Answered: 2019-07-18
Question
We need using your anti-BMAL1/ARNTL antibody for maternal process involved in parturition studies. Has this antibody been tested with western blotting on hela whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
We appreciate your inquiry. This A00260-1 anti-BMAL1/ARNTL antibody is tested on hela whole cell lysates, hepg2 whole cell lysates. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-22
Question
We were happy with the WB result of your anti-BMAL1/ARNTL antibody. However we have seen positive staining in fetal brain nucleus using this antibody. Is that expected? Could you tell me where is ARNTL supposed to be expressed?
G. Krishna
Verified customer
Asked: 2018-06-01
Answer
Based on literature, fetal brain does express ARNTL. Generally ARNTL expresses in nucleus. Regarding which tissues have ARNTL expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9144434, 14702039
Brain, and Skin, Pubmed ID: 15489334
Fetal brain, Pubmed ID: 9079689
Boster Scientific Support
Answered: 2018-06-01
Question
We are currently using anti-BMAL1/ARNTL antibody A00260-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
G. Banerjee
Verified customer
Asked: 2017-11-08
Answer
The anti-BMAL1/ARNTL antibody (A00260-1) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-08
