Overview
| Product Name |
Anti-BMAL1/ARNTL Antibody Picoband™
See more BMAL1 products |
| Catalog# |
A00260-1 |
| Pack Size |
100μg/vial |
| Storage & Handling |
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Description |
Boster Bio Anti-BMAL1/ARNTL Antibody Picoband™ catalog # A00260-1. Tested in WB applications. This antibody reacts with Human. Supplied as 100μg/vial in Lyophilized form antibody. |
| Cite This Product |
Anti-BMAL1/ARNTL Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00260-1)
|
| Antibodies Validation |
Antibodies Validation Information
|
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Anti-BMAL1/ARNTL Antibody Picoband™ may replace the following items: sc 8550|sc 48790|sc 365645|sc 373955|sc 8550 X|sc 48790 X|sc 365645 X|sc 373955 X. |
Product Specs
| Host |
Rabbit |
| Reactive Species |
Human |
| Applications |
WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Related Products |
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $100. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round. |
| Immunogen |
E.coli-derived human BMAL1/ARNTL recombinant protein (Position: H58-D156). Human BMAL1/ARNTL shares 99% and 98% amino acid (aa) sequence identity with mouse and rat BMAL1/ARNTL, respectively. |
| Cross Reactivity |
No cross reactivity with other proteins. |
Gene/Protein Basic Information For ARNTL (Source: Uniprot.org, NCBI)
| Uniprot Id | O00327 |
|---|
| NCBI Gene Id | 406 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | ARNTL |
|---|
| Protein Name | Aryl hydrocarbon receptor nuclear translocator-like protein 1 |
|---|
| Alternative Names | BMAL1|aryl hydrocarbon receptor nuclear translocator-like protein 1; aryl hydrocarbon receptor nuclear translocator-like; basic-helix-loop-helix-PAS orphan MOP3; BHLHE5; bHLHe5brain and muscle; bHLH-PAS protein JAP3; BMAL1TIC; Brain and muscle ARNT-like 1; Class E basic helix-loop-helix protein 5; Member of PAS protein 3; member of PAS superfamily 3; MOP3BMAL1c; PAS domain-containing protein 3; PASD3MGC47515 |
|---|
| Molecular Weight | 68762 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ARNTL, check out the ARNTL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for ARNTL: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the ARNTL infographic
Our Boster Quality Guarantee for Anti-BMAL1/ARNTL Antibody Picoband™ covers its use in the following applications.
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Figure 1. Western blot analysis of BMAL1/ARNTL using anti-BMAL1/ARNTL antibody (A00260-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HELA whole Cell lysates,
Lane 2: HEPG2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMAL1/ARNTL antigen affinity purified polyclonal antibody (Catalog # A00260-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMAL1/ARNTL at approximately 69KD. The expected band size for BMAL1/ARNTL is at 69KD.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Did You Know ?
That Boster Bio offers comprehensive selection of Western blot and IHC reagents? Great quality and save up to 50% cost.
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Questions and answers from customers related to A00260-1 Anti-BMAL1/ARNTL Antibody Picoband™
5 Related Questions
Question
We have observed staining in human brain. Do you have any suggestions? Is anti-BMAL1/ARNTL antibody supposed to stain brain positively?
Verified Customer
Asked: 2019-09-19
Answer
According to literature brain does express ARNTL. According to Uniprot.org, ARNTL is expressed in left lobe of thyroid gland, brain, fetal brain, brain skin, among other tissues. Regarding which tissues have ARNTL expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9144434, 14702039
Brain, and Skin, Pubmed ID: 15489334
Fetal brain, Pubmed ID: 9079689
Boster Scientific Support
Answered: 2019-09-19
Question
We need using your anti-BMAL1/ARNTL antibody for maternal process involved in parturition studies. Has this antibody been tested with western blotting on hela whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Asked: 2019-05-22
Answer
We appreciate your inquiry. This A00260-1 anti-BMAL1/ARNTL antibody is tested on hela whole cell lysates, hepg2 whole cell lysates. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-22
Question
We were happy with the WB result of your anti-BMAL1/ARNTL antibody. However we have seen positive staining in fetal brain nucleus using this antibody. Is that expected? Could you tell me where is ARNTL supposed to be expressed?
G. Krishna
Asked: 2018-06-01
Answer
Based on literature, fetal brain does express ARNTL. Generally ARNTL expresses in nucleus. Regarding which tissues have ARNTL expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9144434, 14702039
Brain, and Skin, Pubmed ID: 15489334
Fetal brain, Pubmed ID: 9079689
Boster Scientific Support
Answered: 2018-06-01
Question
We are currently using anti-BMAL1/ARNTL antibody A00260-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
G. Banerjee
Asked: 2017-11-08
Answer
The anti-BMAL1/ARNTL antibody (A00260-1) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-08
Question
Has A00260-1 Antibody been tested to react on mouse or rat BMAL1?
Answer
The Anti-BMAL1/ARNTL Antibody Picoband A00260-1 didn't work well on mouse and rat samples.
Boster Scientific Support
Answered: 2019-07-18
