Anti-BMAL1/ARNTL Antibody Picoband®

ARNTL antibody

Boster Bio Anti-BMAL1/ARNTL Antibody Picoband® catalog # A00260-3. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A00260-3
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, WB

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Product Name

Anti-BMAL1/ARNTL Antibody Picoband®

View all ARNTL Antibodies

SKU/Catalog Number

A00260-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-BMAL1/ARNTL Antibody Picoband® catalog # A00260-3. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BMAL1/ARNTL Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00260-3)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human BMAL1/ARNTL recombinant protein (Position: R116-E447).

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00260-3 is reactive to ARNTL in Human, Mouse, Rat

Observed Molecular Weight

75 kDa

Calculated molecular weight

68.8 kDa

Background of ARNTL

Aryl hydrocarbon receptor nuclear translocator-like protein 1 is protein that in humans is encoded by the ARNTL gene. The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00260-3 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -

Positive Control

WB: human U87 whole cell, human HT1080 whole cell, human Hela whole cell, human HepG2 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse heart tissue, mouse Neuro-2a whole cell

Validation Images & Assay Conditions

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5 Customer Q&As for Anti-BMAL1/ARNTL Antibody Picoband®

Question

We bought anti-BMAL1/ARNTL antibody for WB on left lobe of thyroid gland in the past. I am using human, and We intend to use the antibody for ELISA next. you antibody examining left lobe of thyroid gland as well as brain skin in our next experiment. Do you have any suggestion on which antibody would work the best for ELISA?

Verified Customer

Verified customer

Asked: 2019-12-16

Answer

I have checked the website and datasheets of our anti-BMAL1/ARNTL antibody and it seems that A00260-3 has been tested on human in both WB and ELISA. Thus A00260-3 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-12-16

Question

Our lab were happy with the WB result of your anti-BMAL1/ARNTL antibody. However we have been able to see positive staining in brain nucleus using this antibody. Is that expected? Could you tell me where is ARNTL supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-06-14

Answer

Based on literature, brain does express ARNTL. Generally ARNTL expresses in nucleus. Regarding which tissues have ARNTL expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9144434, 14702039
Brain, and Skin, Pubmed ID: 15489334
Fetal brain, Pubmed ID: 9079689

Boster Scientific Support

Answered: 2018-06-14

Question

I am interested in using your anti-BMAL1/ARNTL antibody for negative regulation of transcription studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-04-23

Answer

Thank you for your inquiry. This A00260-3 anti-BMAL1/ARNTL antibody is validated on human k562, k562 whole cell lysate, rat heart tissue, tissue lysate, mouse heart tissue. It is guaranteed to work for ELISA, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-04-23

Question

We are currently using anti-BMAL1/ARNTL antibody A00260-3 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2018-04-03

Answer

The anti-BMAL1/ARNTL antibody (A00260-3) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-04-03

Question

We have been able to see staining in mouse brain. Any tips? Is anti-BMAL1/ARNTL antibody supposed to stain brain positively?

N. Krishna

Verified customer

Asked: 2014-12-10

Answer

Based on literature brain does express ARNTL. Based on Uniprot.org, ARNTL is expressed in left lobe of thyroid gland, brain, fetal brain, brain skin, among other tissues. Regarding which tissues have ARNTL expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9144434, 14702039
Brain, and Skin, Pubmed ID: 15489334
Fetal brain, Pubmed ID: 9079689

Boster Scientific Support

Answered: 2014-12-10

Order DetailsPrice
A00260-3

100μg

$370
A00260-3-Fluoro488

100 μg Fluoro488 conjugated (liquid)

$570
A00260-3-Fluoro550

100 μg Fluoro550 conjugated (liquid)

$570
A00260-3-Fluoro594

100 μg Fluoro594 conjugated (liquid)

$570
A00260-3-Fluoro647

100 μg Fluoro647 conjugated (liquid)

$670
A00260-3-APC

100 μg APC conjugated (liquid)

$820
A00260-3-FITC

100 μg FITC conjugated (liquid)

$570
A00260-3-Cy3

100 μg Cy3 conjugated (liquid)

$570
A00260-3-Biotin

100 μg Biotin conjugated (liquid)

$570
A00260-3-HRP

100 μg HRP conjugated (liquid)

$570
A00260-3-PE

100 μg PE conjugated (liquid)

$820
A00260-3-carrier-free

Carrier Free

$370
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Free Secondary Antibody

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$55.00  $110.00
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A00260-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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We promise all of our products perform as described in datasheets.

This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Apr 13, 2026